Shigeru Takemori

Antitumor activity of selective hyperthermia in tumor-bearing rats using thermosensitive magnetoliposomes as a new hyperthermic material

AbstractThe antitumor activity of dextran magnetite (DM)-incorporating thermosensitive liposomes, namely thermosensitive magnetoliposomes (TMs), as a new hyperthermic material was examined in rats bearing AH60C tumors. TMs were injected at an iron concentration of 15 mg per cm3 tumor into AH60C tumors placed subcutaneously in the backs of Donryu rats. After injection, the whole body of the rat (treated group) was exposed to a 500-kHz electromagnetic field generated by inductive heating. The treated group was further divided into once- and twice-treated groups in order to examine whether repetitive hyperthermia is possible after a single TM injection. A control group also received a TM injection but was not treated with hyperthermia. In the heated groups, there was a marked temperature rise inside the tumor up to 42°C within 7 min, but surrounding tissues were not heated. The inhibition of the growth of AH60C tumor in the treated groups was significantly greater than in the control group (P <. 01). Histolo...

Intestinal Flora and Cancer Control: Inhibitory Effect of Oral Administration of Lactobacillus casei in a Model of Hepatic Metastasis of Rat

Studies on the antimetastatic activity of orally administered Lactobacillus casei (L. casei) were performed using an experimental model of hepatic metastasis. Orally administered L. casei (BLP) significantly inhibited liver tumor colonization, and lowered the incidence of hepatic metastasis the most at a dose of 100mg/kg per day. Furthermore, liver tumor colonization after inoculation with AH60C tumor cells was significantly inhibited in all the groups given BLP, particularly in the groups which were given BLP orally before tumor inoculation (P < 0.01). However, BLP had no significant effect on the rate of incidence of hepatic metastasis. These results strongly suggest that L. casei has an antimetastatic effect, and that it may be of therapeutic use for the prevention of micrometastasis after surgery.

The clinical pharmacokinetics of two different preparations of intrarectal Ketoprofen following spinal or local anesthesia for anal surgery

Two different preparations of commercially available suppositories containing Ketoprofen (KP) were administered to 49 patients immediately following anal surgery. The KP was prepared as either fatty suppositories (FS) or gelatin capsulated suppositories (GCS) and surgery was performed under either spinal (n=37) or local anesthesia (n=12). Similar results were observed in the kinetics of KP after both FS and GCS administration. The extent of bioavailability of the two dosage forms in the patient groups and control subjects (n=10) were essentially equal. When the pharmacokinetic parameters of KP were compared between patient groups under spinal and local anesthesia, significant differences were found in the values of the peak level (C max), peak time (T max), and terminal phase half-life (t 1/2). The C max decreased by one-half, while the T max and t 1/2 increased twice and four times, respectively, in patient operated on under spinal anesthesia compared to those operated on under local anesthesia. The absorption rate constant (Ka) following spinal anesthesia was significantly less than that following local anesthesia or that of the healthy subjects (p<0.01). A “flip-flop” phenomena could be seen in the time profiles of plasma KP concentration following spinal anesthesia.

Efficacy of Hyperthermia for Advanced Esophageal Carcinoma

The prognosis of patients with advanced cancer of the esophagus is very poor. To control advanced esophageal carcinoma nonoperatively, irradiation and chemotherapy have been applied. However, the clinical effectiveness of these treatments has not been satisfactory. Hyperthermia has recently received attention since neoplastic cells have been reported to have greater heat-sensitivity than normal cells, and because this method has been confirmed to enhance the anticancer effect of radiation and some anticancer drugs. We have employed multidisciplinary treatment consisting of radiotherapy, chemotherapy, and hyperthermia, since 1985 [1]. This treatment protocol was derived from basic experiments using cultured cells of human cancer of the esophagus. This is a report of our study on the efficacy of hyperthermia in combined treatment.