Shiichi Nishida

On the sperm-like substances in the interstitial tissue of the fowl testis

P32投与実験の成鶏精巣のHEIDENHAIN鉄へーマキトシリン•ライトグリーン染色標本において,所々に精細管基底膜疎性となり,甚しい時は一部欠損を来し,間質は拡大しその結合組織は疎となり大空隙を生じたものが認められた。かかる間質およびその附近の間質内に1~2個から10数個集団をなした形態的に精子頭部に酷似する糸状体を発見した。このものはFEULGEN反応陽性であり精子頭部と考えられるが,頭部構造は明らかでなく,尾部の認められる場合は極めて稀であつた。間質内および精細管基底膜欠損部には精子を含む種々の階程の精細胞が見られ,欠損部附近の精細管内の精上皮の配列は乱れ,間質内の精細胞核には屡々核溶解像が認められた。かかる現象はP32投与鶏対照鶏いずれにおいても,また材料採取方法について見るとbiopsy,autopsyのいずれにおいても認められた。

Influences of hypophysectomy in mice

In order to clarify the normal involutionary changes in the reproductive organs and adrenals which occur as a results of hypophysectomy, a histological study on these organs of hypophysectomized female mice was performed. Twenty immature and 28 mature mice of the bc strain were subjected hypophysectomy and autopsied at various periods ranging from 1 to 150 days after hypophysectomy. the operation the immature mice were 28 to 30 days of age, and the mature ones 2 months of age. autopsy, the organs to be studied were all dissected from the surrounding fatty connective tissue, weighed and prepared for histological studies. The fixatives and staining methods employed were the same as those described in our previous reports, except for some cases in which the organs were fixed in chilled 80% alcohol and stained for alkaline monoglycerophosphatase by Gomoris technic.Following hypophysectomy there was a fall in boby weight. The reproductive organs and adrenal glands regressed in weight and underwent a pronounced atrophy. The changes in organ weights are summarized in tables 1 and 2.Ovaries: A very rapid regression in size of ovaries took place. Thus there has been a loss of some 50% in weight on the 10th day after hypophysectomy. The first structures to show significant changes after hypophysectomy were large and medium sized follicles. Signs of atresia began to be seen as early as 24 hours, showing clumping of chromatin and achromatolysis of the granulosa cells. Later the thecal cells underwent atrophy, the ovum degenerated and on the 5th day no large follicles remained. Small follicles showed a gradual decrease in number and very few were seen on the 25th day, the ovary becoming to be filled with stromal cells. However, growth of the primordial follicles did not cease. They might develop to the beginning stage of antrum formation, because in the granulosa cells of the small follicles, although not numerous, mitosis were often seen. In the ovaries of prepuberally operated mice, pituitary defficiency manifestations were also evident. Since abvanced stages of follicular maturation did not occur in any case. The most interesting fact about alkaline phosphatase which has emerged from this study is that all follicles of varying size develop large quantities of the enzyme in the granulosa layer which is normally almost devoid of activity.Ovarian stroma: The ovarian stroma of the operated mice was composed of a highly cellular tissue with densely staining nuclei. The nuclei underwent a retrogressive process designated as “wheel cell formation”.Corpora lutea: When corpora lutea were present, a process of degeneration began within 5 days and ended by the 20th day after the operation. Corpora lutea of pregnancy in the mice operated at midpregnancy, however, looked quite normal at the end of gestation.Uterus and Vagina: After the operation the uterus atrophied to a small and compact structure, very similar to that found in the spayed animals. The vaginal epithelium underwent a pronounced atrophy and the epithelium consisted of two layers of cells only.Adrenal gland: The adrenal glands underwent cortical atrophy in a marked degree and their individual cells decreased in size. The histological changes produced by hypophysectomy in the adrenal glands were almost the same as those described in our previous papers, but were somewhat prominent in females. It is of considerable interest that after operation alkaline phophatase developed in the adrenal medullary cells, which is normally devoid of activity in them.

The post-natal histogenesis of the lymph node in the domestic duck

孵化後5日から孵化後180日に到る間の家鴨の頸胸淋巴節と腰淋巴節について発生学的研究を行い次の知見を得た。1) 家鴨淋巴節を構成する主な要素は淋巴索, 淋巴濾胞, 辺縁帯, 辺縁帯外側の膠原繊維層である。この膠原繊維層については従来の文献では記載があいまいであったがこれを確認した, 然しこれは非常に薄いので被膜と呼んで良いかは疑問である。2) 孵化後5日では上述の諸構成要素がいずれも存在するが, その構成が簡単である。日令と共に段々と複雑化し, 45日令以後ではあまり変化しない。故に一般構造は30～45日令で成体の域に達すると思われる。3) 30日令までは二次小節は見られないが45日令で濾胞の一部に円形に不明瞭な二次小節が現れる。この時期の二次小節には原形質に富む核の明るい細胞と原形質の少い核の暗い細胞とか混在していて周囲の淋巴組織と画然と別れていない。4) 60日令で二次小節はほぼ明瞭となり. 二次小節を包む繊維性包膜が表れる, この繊維性包膜は哺乳類では見られない所のものである。5) 90日令で二次小節は60日令のものより更に発達し, 二次小節の中の細胞は原形質に富み核は明るくしばしば分裂像を含む, 又二次小節を包む繊維性包膜も60日令のものより更に発達し, 格子繊維, 膠原繊維を含む, 又二次小節の中の格子繊維は二次小節以外の所に比してその数少く微細である。90日令以後は大した変化なく只二次小節の数が増大した。

On the abnormal mitosis in the testis of domestic duck

雄北京アヒル37羽を使用しその精子発生状態を季節的に追究し次のような結果を得た。1 睾丸重量は5～8月に最大で9月以降減少し,3月頃から増大し,細精管口径もほゞ平行して変化する。2 5～8月に於て最も旺盛な精子発生状態を示,9月以降に於ては退化像を示し精上皮は層状に細精管内腔に脱落する。3 10月及3～4月頃の睾丸に於ては精細胞にPycnosis等の退化像も多数認められ,細精管上皮は精祖細胞を主体とし精母細胞は少数であり,精祖細胞には分裂像を認める。4 これらの時期の精祖細胞に於て少数ではあるが,多核巨大細胞,染色体橋,染色体遅延分断,多極分裂等の異常を認めた。稿を終るに当り終始御懇切な御指導及び御忠言を賜つた恩師増井清先生に衷心から御礼を申し上げる。尚本研究は文部省科学研究費によつて行つたものの一部であることを記して感謝の意を表する。