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Featured researches published by Shin Sakuma.


Journal of Microbiological Methods | 1984

A new culturing method for production of filamentous hemagglutinin of Bordetella pertussis

Atsushi Imaizumi; Yoji Suzuki; Akihiro Ginnaga; Shin Sakuma; Yuji Sato

Abstract The addition of cyclodextrins, especially heptakis (2,6- O -dimethyl)β-cyclodextrin (MeβCD), to synthetic medium, such as Stainer-Scholte medium (D.W. Stainer and M.J. Scholte, J. Gen. Microbiol. 63: 211–220) greatly stimulated the production of filamentous hemagglutinin (F-HA) by Bordetella pertussis Tohama phase I under shaking conditions. Purified F-HA from this culturing system was demonstrated immunochemically and morphologically to be identical with that from static culture.


Archive | 1984

Method for the production of HA fraction containing protective antigens of Bordetella pertussis and pertussis vaccine

Akihiro Ginnaga; Hiroshi Koba; Shin Sakuma; Hisashi Kitagawa; Akira Yamada; Yoji Suzuki


Archive | 1984

Method for the purification of LPF-HA

Shin Sakuma; Kuniaki Sakamoto; Hisashi Kitagawa; Mitsuo Sakoh; Saneo Nonaka


Archive | 1985

Method for purification of filamentous hemagglutinin

Akihiro Ginnaga; Shin Sakuma; Tsukasa C O Juridica Nishihara; Tomitaka Tashiro; Sadao Susumi; Tetsuo Kawahara; Hiroshi Mizokami


Archive | 1985

Method for the production of pertussis component vaccine and combined vaccine of pertussis antigen; diphtheria toxoid and tetanus toxoid

Akihiro Ginnaga; Mitsuo Sakoh; Hisashi Kitagawa; Shin Sakuma; Hiroshi Koba; Tsukasa C; Sadahiro Hirashima


Archive | 1985

Méthode de production d'un composant de vaccin contre la coqueluche et vaccin combiné d'antigène de coqueluche, de toxoide de la diphtérie et de toxoide du tétanos

Akihiro Ginnaga; Mitsuo Sakoh; Hisashi Kitagawa; Shin Sakuma; Hiroshi Koba; Tsukasa C; Sadahiro Hirashima


Archive | 1985

A process for purifying fibrous hemoglobin.

Akihiro Ginnaga; Shin Sakuma; Tsukasa C O Juridica Nishihara; Tomitaka Tashiro; Sadao Susumi; Tetsuo Kawahara; Hiroshi Mizokami


Archive | 1984

Verfahren zur reinigung von lpf-ha. A method for cleaning lpf-ha.

Shin Sakuma; Kuniaki Sakamoto; Hisashi Kitagawa; Mitsuo Sakoh; Saneo Nonaka


Archive | 1984

Verfahren zur herstellung der bordetella-pertussis-schutzantigene enthaltenden ha fraktion und keuchhustenvakzin. Process for the preparation of bordetella pertussis protection antigenic fraction containing ha and keuchhustenvakzin.

Akihiro Ginnaga; Hiroshi Koba; Shin Sakuma; Hisashi Kitagawa; Akira Yamada; Yoji Suzuki


Archive | 1984

Verfahren zur reinigung von lpf-ha.

Shin Sakuma; Kuniaki Sakamoto; Hisashi Kitagawa; Mitsuo Sakoh; Saneo Nonaka

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