Shinnosuke Tanaka

A human gall‐bladder signet ring cell carcinoma cell line

To date, very few reports of the establishment of gall‐bladder cancer cell lines have appeared,1–7 although many cancer cell lines of various kinds have been established. On the other hand, no reports could be found on signet ring cell carcinoma cell lines derived from the gall‐bladder and only five cell lines from the stomach. A human gall‐bladder cancer cell line (FU‐GBC‐2) was established In tissue culture from the ascitic fluid of a 69‐year‐old Japanese female patient. The tumor cells growing In tissue culture exhibited the morphological characteristics of signet ring cells In phase contrast and electron microscopy. The population doubling time was 43 hours. Heterotransplantation was succeeded by inoculation into me dermis of BALB/c nude mice. An Immunocytochemical study showed that most of the cultured cells were positive for carclnoembryonic antigen, CA19–9 and epithellal membrane antigen, but negative for vimentin. The modal chromosome number was 120 with a range of 100–124. Flow cytometry showed an aneuploidy pattern in the cultured cells at passage 30. Markedly amplified c‐myc oncogene was observed by Southern blot analysis. This cell line may be useful In the study of the morphological and biological characteristics of signet ring cell carcinoma and gallbladder adenocarcinoma.

Early Start of Chemotherapy after Resection of Primary Colon Cancer with Synchronous Multiple Liver Metastases: A Case Report

The start of chemotherapy treatment usually requires a delay of about 4 weeks after surgical resection in patients with primary colorectal cancer and synchronous distant metastasis. However, there is no evidence to indicate the required length of this delay interval. In addition, there is a chance that a patient may die because postoperative chemotherapy was not started soon enough and a metastatic tumor was able to develop rapidly. Here, we present a case in which combination chemotherapy with capecitabine and oxaliplatin (XELOX) was started within 1 week after a right hemicolectomy for synchronous multiple liver metastases. To our knowledge, this is the first report of the start of chemotherapy, involving treatments such as folinic acid, fluorouracil, and oxaliplatin (FOLFOX); folinic acid, fluorouracil, and irinotecan (FOLFIRI); and XELOX, within 1 week after a colorectal cancer operation with anastomosis. The findings suggest possible changes in the start time of chemotherapy after surgery in the future.

MK-1 Expression in Gastric Carcinoma with Liver Metastasis

OBJECTIVE The prognosis for gastric carcinoma patients with liver metastasis is very poor. This retrospective study investigated the prognostic significance of MK-1 expression in gastric carcinoma patients with liver metastasis. METHODS Immunohistochemical staining using monoclonal antibody FU-MK-1 against MK-1 antigen was performed on paraffin-embedded tissues from 64 gastric carcinoma patients with liver metastasis. We attempted to determine the presence of any relationship between pathological prognostic factors and the expression of MK-1 in 64 gastric carcinoma patients with liver metastasis. RESULTS MK-1 expression was found in 43 (67%) of 64 tumor samples. MK-1 expression was significantly higher in the intestinal type (73%) than in the diffuse type carcinoma (33%, P = 0.049). Multivariate analysis showed that MK-1 expression and lymph node metastasis were significant factors for overall survival. The difference between overall survival rates with positive or negative MK-1 expression was statistically significant as shown by Kaplan-Meier survival analysis (P < 0.0001; log-rank). In addition, the difference between cumulative disease-free survival rates with positive or negative MK-1 expression in gastric carcinoma patients with metachronous liver metastasis was statistically significant as well, as shown by Kaplan-Meier survival analysis (P = 0.0006; log-rank). CONCLUSIONS The prognostic significance of MK-1 expression as a biological tumor marker was demonstrated in a series of gastric carcinoma patients with liver metastasis. MK-1 positivity may be a reliable marker for predicting and taking measures to control liver metastasis after curative gastrectomy for gastric carcinoma.

Study of Lymphatic Flow in the Remnant Stomach by Endoscopical Injection of Activated Carbon Particles(CH40).

残胃の癌症例に微粒子活性炭CH40を術前経内視鏡的に残胃小轡に流入し, リンパ節の黒染の有無により残胃のリンパ流を検討した. 対象は14例で, 初回手術は良性疾患6例 (B-1法3例, B-2法3例). 悪性疾患8例 (B-1法5例, B-2法3例) であった. 初回良性例ではNo.1, 3, 7のリンパ節が黒染され左胃動脈経路が主体であったが, No.10, 11などの脾動脈経路のリンパ節も黒染されていた. 初回悪性例のR2郭清後では左胃動脈経路はみられず, 脾動脈経路, 左下横隔動脈経路が主体であったが, B-1法ではNo.12, 13への新生リンパ路がみられた. R3郭清後では既成リンパ路の遮断により, B-1法でNo.16のみ, B-2法では空腸間膜リンパ節のみの黒染例がみられ, いずれも新生リンパ路であった. 初回悪性例では残胃副リンパ路のリンパ流の増加および新生リンパ路がみられたが, R2とR3の郭清後では異なったリンパ流がみられた.