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Analytical Letters | 1986

Highly Sensitive Sandwich Enzyme Immunoassay for Human Thyroid-Stimulating Hormone (hTSH) in Serum Using Monoclonal Anti-hTSH β-Subunit IgG1-Coated Polystyrene Balls and Polyclonal Anti-Human Chorinic Gonadotropin Fab′-Horseradish Peroxidase Conjugate

Shinobu Inoue; Seiichi Hashida; Eiji Ishikawa; Toru Mori; Hiroo Imura; Hiroshi Ogawa; Takafumi Ichioka; Katsuyuki Nakajima

Abstract A highly sensitive sandwich enzyme immunoassay for human thyroid-stimulating hormone (hTSH) in serum is described. Monoclonal anti-hTSH β-subunit IgG1-coated polystyrene balls were incubated with serum samples and subsequently with polyclonal anti-human chorionic gonadotropin Fab1-horseradish peroxidase conjugate. Neither hTSH nor anti-hTSH serum was required except for a small amount of hTSH as a standard. The detection limit of hTSH was 0.1 nU/tube or 0.005 mU/1 for most serum samples except for those from pregnant women, since there was no cross-reaction with 209 IU/1 of human follicle-stimulating hormone and 439 IU/1 of human luteinizing hormone when 20 μ1 of serum sample was used. Serum hTSH levels in normal subjects and Graves1 disease were 1.22 ± 0.70 (SD) mU/1 (range: 0.26–2.65 mU/1) and 0.080 ± 0.062 (SD) mU/1 (range: < 0.005–0.17 mU/1), respectively.


Annals of Clinical Biochemistry | 2016

Development of a novel ultrasensitive enzyme immunoassay for human glutamic acid decarboxylase 65 antibody

Satoshi Numata; Hideki Katakami; Shinobu Inoue; Hirotake Sawada; Seiichi Hashida

Background We developed a novel, ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for determination of glutamic acid decarboxylase autoantibody concentrations in serum samples from patients with type 2 diabetes. Methods We developed an immune complex transfer enzyme immunoassay for glutamic acid decarboxylase autoantibody and measured glutamic acid decarboxylase autoantibody from 22 patients with type 1 diabetes, 29 patients with type 2 diabetes, and 32 healthy controls. Results A conventional ELISA kit identified 10 patients with type 1 diabetes and one patient with type 2 diabetes as glutamic acid decarboxylase autoantibody positive, whereas 15 patients with type 1 diabetes and six patients with type 2 diabetes were identified as glutamic acid decarboxylase autoantibody positive using immune complex transfer enzyme immunoassay. Conclusions Immune complex transfer enzyme immunoassay is a highly sensitive and specific assay for glutamic acid decarboxylase autoantibody and might be clinically useful for diabetic onset prediction and early diagnosis.


Journal of Biochemistry | 1985

A Micro-Scale Method for the Conjugation of Affinity-Purified Fab' to β-D-Galactosidase from Escherichia coli

Shinobu Inoue; Seiichi Hashida; Takeyuki Kohno; Koichiro Tanaka; Eiji Ishikawa


Analytical Letters | 1985

Preparation of Monomeric Affinity-Purified Fab'-ß-D-Galactosidase Conjugate for Immunoenzymometric Assay

Shinobu Inoue; Seiichi Hashida; Koichiro Tanaka; Masayoshi Imagawa; Eiji Ishikawa


Clinical Biochemistry | 2012

Development of an ultra-sensitive enzyme immunoassay for human insulin autoantibodies.

Satoshi Numata; Asako Umehara; Hideki Katakami; Shinobu Inoue; Seiichi Hashida


Journal of Clinical Laboratory Analysis | 1991

Time-Resolved fluorometric sandwich immunoassay for human growth hormone in serum and urine

Seiichi Hashida; Koichiro Tanaka; Eiji Ishikawa; Shinobu Inoue; Kunio Hayakawa


Analytical Letters | 1986

Highly Specific and Sensitive Sandwich Enzyme Immunoassay for Human Thyroid-Stimulating Hormone (hTSH) in Serum Using Monoclonal Anti-hTSH β-Subunit IgG1-Coated Polystyrene Balls and Polyclonal Anti-hTSH β-Subunit Fab′-Horseradish Peroxidase Conjugate

Seiichi Hashida; Shinobu Inoue; Eiji Ishikawa; Toru Mori; Hiroo Imura; Hiroshi Ogawa; Takafumi Ichioka; Katsuyuki Nakajima


Analytical Letters | 1984

A Small Scale Preparation of Affinity-Purified Rabbit Fab'-Horseradish Peroxidase Conjugate for Enzyme Immunoassay

Shinobu Inoue; Masayoshi Imagswa; Seiichi Hashida; Ke-He Ruan; Eiji Ishikawa


Endocrine Journal | 1998

Peripheral plasma levels of human growth hormone releasing hormone (GHRH) during the sleep test in short children

Shinobu Inoue; Hideki Katakami; Hiroyuki Hidaka; Seiichi Hashida; Eiji Ishikawa; Shigeru Matsukura; Tohru Sugimoto


Archive | 1987

Enzyme Labeling of Antibodies and Enzyme Immunoassay

Eiji Ishikawa; Seiichi Hashida; Takeyuki Kohno; Koichiro Tanaka; Ke-He Ruan; Shinobu Inoue

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Seiichi Hashida

Tokushima Bunri University

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Eiji Ishikawa

Shiga University of Medical Science

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Hiroshi Ogawa

National Institute of Advanced Industrial Science and Technology

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Satoshi Numata

Health Science University

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