Shinsuke Ohno

Nonrandom chromosome changes involving the Ig gene-carrying chromosomes 12 and 6 in pristane-induced mouse plasmacytomas

The karyotypes of pristane-induced mouse plasmacytomas were studied by G banding. Only primary tumors or early passage generations were analyzed. In contrast to murine T cell leukemias that showed a regular trisomy of chromosome 15, all plasmacytomas showed a consistent translocation of the distal part of chromosome 15 to either chromosome 6 [rcpT(6;15)] or 12 [T(12;15)]. The specific breakpoints were at 6C, 15D3/E ro D2/3 and 12F2. Early passage generations often showed a mixed population with two different translocations, suggesting polyclonal origin. Considered together with the known karyotypic features of murine and human lymphomas, these findings support the theory that the nonrandom chromosomal changes in lymphoproliferative malignancies are associated with the type of the target cell, rather than with the etiological agent. Moreover, the involvement of the chromosomes known to carry the heavy chain (12) and the light chain (6) determinants, respectively, raises the question of whether the translocations may be related to the DNA level rearrangements known to occur during the differentiation of normal plasma cells.

Serological reactivities of human and baboon sera against EBNA and Herpesvirus papio-determined nuclear antigen (HUPNA)☆

Abstract Using our recently developed acid-fixed nuclear binding technique, an EBNA-like antigen was detected in both virus-producer and non-producer baboon lymphoid cell lines that carried Herpesvirus papio (HVP. We designated the antigen as HUPNA. Serological comparison of EBNA and HUPNA showed that: ( i ) all EBNA-positive sera reacted with both EBNA and HUPNA; ( ii ) none of the anti-HVP(VCA)-positive baboon sera reacted with EBNA; and ( iii ) approximately half of the anti-HPV(VCA)-positive baboon sera tested reacted with HUPNA. HUPNA could not be visualized by anticomplement immunofluorescence in situ .

Relationship between Herpesvirus ateles-associated Nuclear Antigen (HATNA) and the Number of Virus Genome Equivalents in HVA-carrying Lymphoid Lines

A DNA-binding antigen (HATNA) was demonstrated in 7 out of 14 cell lines carrying Herpesvirus ateles (HVA) by the acid-fixed nuclear-binding technique. The seven HATNA-positive lines had means of 95, 96, 103, 177, 240, 343 and 326 virus genome equivalents/cell. For the seven HATNA-negative lines, the figures were 4, 8, 10, 33, 39, 72 and 110. This indicates a relationship between the number of HVA genome equivalents/cell and the detectability of HATNA. This association was independent of the virus producer status of the lines.

Evidence for antigenic distinctness of the Epstein--Barr virus-determined nuclear antigen and the Herpesvirus papio-determined nuclear antigen.

Previous serological evidence indicated that Epstein--Barr virus-determined nuclear antigen (EBNA) has cross reactive components with the herpesvirus papio-determined nuclear antigen (HUPNA) and, in addition, it has distinct components of its own, not present in HUPNA. In the present study, this hypothesis was studied by absorption experiments. Absorption of anti-EBNA positive human sera with EBNA abolished both anti-EBNA and anti-HUPNA reactivity. Absorption with HUPNA removed all anti-HUPNA reactivity but did not reduce anti-EBNA to any detectable degree, thus confirming the hypothesis.