Shinya Kuno

Training-induced changes in muscle architecture and specific tension

Five men underwent unilateral resistance training of elbow extensor (triceps brachii) muscles for 16 weeks. Before and after training, muscle layer thickness and fascicle angles of the long head of the triceps muscle were measured in vivo using B-mode ultrasound, and fascicle lengths were estimated. Series anatomical cross-sectional areas (ACSA) of the triceps brachii muscle were measured by magnetic resonance imaging, from which muscle volume (Vm) was determined and physiological cross-sectional area (PCSA) was calculated. Elbow extension strength (isometric; concentric and eccentric at 30, 90 and 180°·s−1) was measured using an isokinetic dynamometer to determine specific tension. Muscle volumes, ACSA, PCSA, muscle layer thickness and fascicle angles increased after training and their relative changes were similar, while muscle and fascicle length did not change. Muscle strength increased at all velocities; however, specific tension decreased after training. Increase in fascicle angles, which would be the result of increasedVm and PCSA, would seem to imply the occurrence of changes in muscle architecture. This might have given a negative effect on the force-generating properties of the muscles.

Muscle metabolism during exercise using phosphorus-31 nuclear magnetic resonance spectroscopy in adolescents.

Very little has been reported on muscle energetics during exercise in adolescents. This is attributable to the difficulty of subjecting children to muscle biopsy. The purpose of this study was to investigate the characteristics of muscle metabolism during exercisein vivo in adolescents by comparing firstly, with adults and secondly, the differences resulting from physical activity using phosphorus-31 nuclear magnetic resonance (31PNMR) spectroscopy. The subjects were boys aged 12 to 15 years, comprising 21 trained boys and 23 control boys, and 6 adults controls. The ratio of phosphocreatine (PCr):(PCr + Pi), where Pi is inorganic phosphate intracellular pH at exhaustion and the time constant of PCr during recovery were measured in all the subjects using31PNMR. Both groups of children showed higher values of PCr:(PCr + Pi) and intracellular pH at exhaustion than did the adult control group (P < 0.01 orP < 0.05). However, no significant differences were found between the trained boys and the control boys with respect to PCr:(PCr + Pi) and intracellular pH at exhaustion. On the other hand, we found the same values for PCr time constant in all groups. This result suggested no differences of the muscle oxidative capacity between children and adults. We concluded that the adolescents, aged 12 to 15 years in both the trained and control groups, had less glycolytic ability during exercise than the adults.

Changes in magnetic resonance images in human skeletal muscle after eccentric exercise

To investigate the time-course of changes in transverse relaxation time (T2) and cross-sectional area (CSA) of the quadriceps muscle after a single session of eccentric exercise, magnetic resonance imaging was performed on six healthy male volunteers before and at 0, 7, 15, 20, 30 and 60 min and 12, 24, 36, 48, 72 and 168 h after exercise. Although there was almost no muscle soreness immediately after exercise, it started to increase 1 day after, peaking 1–2 days after the exercise (P<0.01). Immediately after exercise, T2 increased significantly in the rectus femoris, vastus lateralis and intermedius muscles (P<0.05) and decreased quickly continuing until 60 min after exercise. At and after the 12th h, a significant increase was perceived again in the T2 values of the vastus lateralis and intermedius muscles (P<0.01) [maximum 9.3 (SEM 2.8)% and 10.9 (SEM 2.2)%, respectively]. The maximal values were exhibited at 24–36 h after exercise. In contrast, the rectus femoris muscle showed no delayed-stage increase. Also, in CSA, an increase after 12 h was observed in addition to the one immediately after exercise in the vastus lateralis, intermedius and medialis and quadriceps muscles as a whole (P < 0.01), reaching the maximal values at 12–24 h after exercise. The plasma creative kinase activity remained unchanged up to 24 h after and then increased significantly 48 h after exercise (P < 0.05). Beginning 12 h after exercise, the subjects whose T2 and CSA increased less than the others displayed a faster decrease in muscle soreness. These results suggested that T2 and CSA displayed bimodal responses after eccentric exercise and the time-courses of changes in them were similar to those in muscle soreness.

Influence of two different modes of resistance training in female subjects

In resistance training, it has been empirically accepted that muscle hypertrophy is developed by low intensity and high volume training, while muscle strength and power are developed by high intensity and low volume training. The purpose of the present study was to investigate the influence of two different modes of resistance training on isokinetic strength and muscle cross-sectional area (CSA) in females. Eleven females, who had no experience in resistance training, participated in this study and were randomly divided into two groups. The former consisted of 4-5 sets of 15-20 RM (repetition maximum) with sufficient rest between sets (Group H), while the latter consisted of 8-9 sets of 4-6R M with 90 s of rest between sets (Group S). The former was assumed to be appropriate for muscle hypertrophy and the latter muscle strength, respectively. All subjects completed isotonic knee extension exercise three times a week for 8 weeks. Measurements were made on quadriceps muscle cross-sectional area (CSA) and isokinetic torques at 0, 60, 180, and approximately 300 degrees before training, at the fifth week and the end of training period. Muscle CSA was defined as the sum of CSA measured at 30, 50 and 70% of femur length. After training, muscle CSA had significantly increased in both groups: 3.3 +/- 0.7% (p < .05) for group H and 3.6 +/- 1.1% (p < .05) for group S, respectively. While the changes in isokinetic torque were 43.4 +/- 47.5% (p < .05) for group H and 27.4 +/- 31.3% (p < .05) for group S, respectively. In both groups the percentage changes of the isokinetic strength were significantly higher than those of the CSA. No significant difference in these variables were found between the two groups. These results suggest that during the early phase of resistance training two different modes of resistance training may have similar effects on muscle CSA and isokinetic strength in untrained females.

Measurement of muscle fibre displacement during contraction by real-time ultrasonography in humans

The contact point (P) made by both the echoes of the aponeurosis and from interspaces among fascicles of the tibialis anterior muscle was detected by real-time ultrasound scanning in 12 adults. Movement in the location of P was observed during muscle contraction and its displacement was related to changes in ankle joint angles (r = 0.81,P < 0.01), i.e. P shifted proximally when the ankle joint was dorsiflexed. There was also a significant positive correlation between the degree of dorsiflexion and the velocity related to the change in location of P (r = 0.84,P < 0.01). Ultrasound measurements of the displacement and the velocity of P were reproducible as there was no variation noticed among measurements on different days. It is suggested from these results that the displacement of P reflected changes in muscle length during contraction and that this amount of change corresponded to changes in joint angles.

Muscle energetics in short-term training during hypoxia in elite combination skiers

AbstractFour well-trained combination skiers were studied through pre- and post-training for the effects of short-term intermittent training during hypoxia on muscle energetics during submaximal exercise as measured by Phosphorus-31 nuclear magnetic resonance and maximal aerobic power (

Muscle architecture and function in humans

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RELATIONSHIP BETWEEN DECREMENT OF MUSCULAR pH AND CO2 EXCESS DURING INCREMENTAL EXERCISE

O2max). The hypoxia and training in the cold was conducted in a hypobaric chamber and comprised 60-min aerobic exercise (at an intensity equivalent to the blood lactate threshold), using a cycle ergometer or a treadmill twice a day for 4, consecutive days at 5°C, in conditions equivalent to an altitude of 2000 m (593 mm Hg). No change in