Shree Bhaskaran

Serial estimation of Th1:th2 cytokines profile in women undergoing in-vitro fertilization-embryo transfer.

To investigate changes in the ratio of T‐cell subpopulations expressing intracellular T helper1 (Th1) and T helper 2 (Th2) cytokines in women with a history of recurrent failed implantation under going in‐vitro fertilization (IVF)‐embryo transfer.

Prednisolone suppresses NK cell cytotoxicity in vitro in women with a history of infertility and elevated NK cell cytotoxicity.

To evaluate the effect of prednisolone on NK cell cytotoxicity in vitro environment and also to compare the effect of prednisolone versus immunoglobulin‐G (IVIG) on NK cell cytotoxicity using in vitro co‐culture with K562 cells.

ORIGINAL ARTICLE: Serial Estimation of Th1:Th2 Cytokines Profile in Women Undergoing In‐Vitro Fertilization‐Embryo Transfer

To investigate changes in the ratio of T‐cell subpopulations expressing intracellular T helper1 (Th1) and T helper 2 (Th2) cytokines in women with a history of recurrent failed implantation under going in‐vitro fertilization (IVF)‐embryo transfer.

ORIGINAL ARTICLE: Prednisolone Suppresses NK Cell Cytotoxicity In Vitro in Women With a History of Infertility and Elevated NK Cell Cytotoxicity

To evaluate the effect of prednisolone on NK cell cytotoxicity in vitro environment and also to compare the effect of prednisolone versus immunoglobulin‐G (IVIG) on NK cell cytotoxicity using in vitro co‐culture with K562 cells.

The Relationship of Systemic TNF‐α and IFN‐γ with IVF Treatment Outcome and Peripheral Blood NK Cells

To evaluate the association of serum tumour necrotic factor (TNF)‐α and interferon (IFN)‐γ levels with IVF treatment outcome and peripheral blood NK cells.

The Effect of Serum Concentration of Leukaemia Inhibitory Factor on In Vitro Fertilization Treatment Outcome

Problem To evaluate the association of peripheral leukaemia inhibitory factor (LIF) levels on implantation and miscarriage rates after in vitro fertilization (IVF) treatment.

ORIGINAL ARTICLE: Prednisolone Suppresses NK Cell Cytotoxicity In Vitro in Women With a History of Infertility and Elevated NK Cell Cytotoxicity: PREDNISOLONE SUPPRESSES NK CELL CYTOTOXICITY IN VITRO

To evaluate the effect of prednisolone on NK cell cytotoxicity in vitro environment and also to compare the effect of prednisolone versus immunoglobulin‐G (IVIG) on NK cell cytotoxicity using in vitro co‐culture with K562 cells.

Patients with Chronic Sarcoidosis have Reduced CD27+IgM+IgD+ Unswitched Memory B cells and an Expanded Population of Terminal Effector CD8+CD27-CD28- T cells

Granulomas that occur in sarcoidosis are histologically identical to those found in a subset of Common Variable Immunodeficiency (CVID) patients where they are associated with a reduction in class switched memory B lymphocytes. The aim of this study was to investigate whether the abnormalities in peripheral blood lymphocyte populations associated with granulomatous variant CVID (gvCVID) are also present in individuals with sarcoidosis. We examined B lymphocyte populations using flow cytometry and found that the reduction of class switched memory (CSM: CD19+CD27+IgM-IgD-) and unswitched memory (CD19+CD27+IgM+IgD+) B cells in our sarcoidosis cohort was similar to that previously reported in gvCVID patients. The reduction of class switched memory B cells in sarcoidosis patients indicated a possible defect in the T cell repertoire as antibody class switching requires T cell help. We subsequently explored the peripheral blood T cell compartment of our sarcoidosis patients. The results identified a population of terminally differentiated effector CD8 + T cells (CCR7 - CD45RA - CD127 - CD27 - CD28 - ) that were significantly expanded in the peripheral blood of sarcoidosis patients. Terminally differentiated effector CD8 + T cells have been defined as cytolytic, inflammatory cells with reduced replicative capacity. The discovery of abnormal peripheral blood B and T cells compartments in sarcoidosis may be of value in clinical diagnosis and could be relevant to the pathogenic process. Patients with Chronic Sarcoidosis have Reduced CD27 + IgM + IgD +

ORIGINAL ARTICLE: Serial Estimation of Th1:Th2 Cytokines Profile in Women Undergoing In-Vitro Fertilization-Embryo Transfer: SERIAL ESTIMATION OF TH1:TH2 IN WOMEN UNDERGOING IVF

To investigate changes in the ratio of T‐cell subpopulations expressing intracellular T helper1 (Th1) and T helper 2 (Th2) cytokines in women with a history of recurrent failed implantation under going in‐vitro fertilization (IVF)‐embryo transfer.