Shri Krishna Tewari

SIMULTANEOUS SEPARATION AND QUANTIFICATION OF TARGETED GROUP OF COMPOUNDS IN PSORALEA CORYLIFOLIA L. USING HPLC-PDA-MS-MS

A rapid, sensitive, and simple high performance liquid chromatography photo diode array mass spectrometry (HPLC-PDA-MS-MS) method was developed for the simultaneous determination of nine compounds, namely, psoralen (furocoumarin), daidzein, genistein (isoflavonoids), daidzin and genestin (isoflavone glycosides), biochanin A (methoxylated isoflavone), and quercetin, kaempferol, and rutin (flavonols). Chromatographic separation of these nine molecules was performed on RP C18 column within 20 min. Elution was accomplished by the application of trifluoroacetic acid (0.05%) in water and methanol in gradient system with flow rate of 1.0 mL/min. PDA and mass spectrometry was employed for qualitative and quantitative analysis. The method is economical in terms of the time expended and the amount of solvent used for each analysis. The calibration curves were linear and ranged between 0.994 to 0.999; limit of detection (LOD) and quantification (LOQ) ranged between 1.02 to 1.18 and 2.23 to 3.34 and recovery ranged between 91.76% to 97.41%. The validated method was successively used to separate and quantitative measurement of these compounds in plant parts of Psoralea corylifolia. The developed method may be used in the quality control and standardization of plant extracts as well as herbal drugs and formulations. Supplemental materials are available for this article. Go to the publishers online edition of Journal of Liquid Chromatography & Related Technologies to view the free supplemental file.

Fatty acid composition and antibacterial potential of Cassia tora (leaves and stem) collected from different geographic areas of India

The comparative analysis of the fatty acid composition of Cassia tora (leaves and stem) was determined using gas chromatography-mass spectrometry. Twenty-seven fatty acids were identified in C. tora (leaves and stem) which was collected from three different geographical areas of India: Lucknow (Uttar Pradesh), Nainital (Uttarakhand), and Bhavnagar (Gujarat), coded as CT-1, CT-2, and CT-3, respectively. The gas chromatography-mass spectrometry analysis showed the presence of various saturated and unsaturated fatty acids. The major fatty acids found were palmitic acid, linoleic acid, linolenic acid, margaric acid, melissic acid, and behenic acid. The highest amounts of saturated fatty acids were found in leaves of C. tora collected from Bhavnagar (Gujarat) (60.7% ± 0.5%). Thus, the study reveals that C. tora has a major amount of nutritionally important fatty acids, along with significant antimicrobial potential. Fatty acids play a significant role in the development of fat products with enhanced nutritional value and clinical application. Remarkable differences were found in the present study between fatty acid profiles of C. tora collected from different locations in India. To the best of our knowledge there is no previously reported comparative study of the fatty acids of C. tora.

Simultaneous quantification of six phenolic compounds in various parts of Moringa oleifera Lam. using high-performance thin-layer chromatography

Fresh pods of Moringa oleifera with nutraceutical importance are widely consumed in food commodities as vegetables. It is nutritious and it also has several biological activities. In the present study, a simple, rapid, cost-efective, and sensitive high-performance thin-layer chromatography (HPTLC) method was applied for the simultaneous determination of six phenolic compounds, viz., gallic (phenolic acid), p-coumaric, cafeic acid (hydroxycinnamic acid), chlorogenic acid (cinnamic acid derivative), quercetin and kaempferol (flavonols) in flowers, pods, leaves, twigs, and seeds of M. oleifera. Simultaneous separation and quantification of compounds were achieved on HPTLC pre-coated silica gel 60 F254 aluminum plates using the mobile phase toluene—ethyl acetate—formic acid (14:10:1). Densitometric determination was carried out at λmax 282 nm. The calibration curves were linear, ranged between 0.984 and 0.998; the limit of detection and quantification ranged between 110.8 ng mL-1 and 142.3 ng mL-1, and 301.6 ng µL-1 and 410.8 ng µL-1; and recovery ranged between 96.2% and 97.9%. The validated method was successively used to analyze the above compounds in the plant parts of M. oleifera. The amount of the total phenolic content and specifc phenolic compounds ranged from 4.86 mg g-1 (gallic acid equivalent [GAE]) to 14.79 mg g-1 (GAE) and 0.007% quercetin (fower and fower with pods) to 0.099% gallic acid (pods of 15 days). This study reveals that the presence of specific phenolic compounds in M. oleifera shall be a good source for the isolation of the above-mentioned compounds for industrial use.

Medicinal value of Cyperus rotundus Linn: An updated review

Cyperus rotundus L. also known as Nagarmotha, purple nut sedge or nut grass, is a common perennial weed plant. It has wide range of medicinal and pharmacological applications, due to which it is widely used as traditional medicine across worldwide to treat various diseases and ailments such as indigestion, constipation, dysentery, abdominal distention, neurogenic gastralgia, chest pains, irregular as well as painful catamenia, skin diseases, furuncle infections, staphylococcal infections, leprosy, sprains and bruises, fever and animal tissue stomach ache. It has numerous range of pharmacological properties like antibacterial and microbial activity, antiplatelet activity, gastrointestinal activity, anti-inflammatory, anti-laceration, wound healing activity, insecticidal properties, anti-rumotoid, anti-diabetic, anti-cancer, anti-malarial, antioxidant, anti-obesity, hepeto-protective activity, anti-diarrheal activity, antispatic activity and anti-pyretic activity. It also has good nutritive values. This communication provides overall review on various medicinal and pharmacological properties of C. rotundus, a noxious weed.

Genetic studies in relation to improvement of gladiolus grown in partially reclaimed sodic soil

Gladiolus (Gladiolus grandiflorus L.) is one of the economically important and common flowering plants largely cultivated worldwide. It is very popular cut flower, because of its many forms and colours. The aim of this study was to evaluate the performance of 25 gladiolus varieties in partially reclaimed sodic soils of Uttar Pradesh to find out the most suitable variety to utilize the degraded waste land. The extent of variability, heritability and correlation coefficients for its floral characters were determined. High heritability coupled with high genetic advance and coefficient of variability were observed for different growth and floral attributes like plant height, plant diameter, tillers per clump, spike length, florets per spike, corm weight and corms per clump. Spike length was positively correlated with all the characters like plant height, leaf length, leaf width and average corm weight except tillers per clump at genotypic level. Number of florets per spike was significantly and positively correlated with plant diameter, leaf width, tillers per clump and corms per clump. The maximum leaf length and width were noticed in White Goddess and Green Woodpecker, respectively. However, maximum average number of cormels per clump was obtained from J V Gold and maximum average weight of cormels was obtained from Friendship. Spike length was positively and significantly associated with the plant height, leaf length & width and average corm weight.