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Featured researches published by Shu-Chen Kan.


Bioresource Technology | 2013

Lipase-immobilized biocatalytic membranes for biodiesel production.

Chia-Hung Kuo; Li-Ting Peng; Shu-Chen Kan; Yung-Chuan Liu; Chwen-Jen Shieh

Microbial lipase from Candida rugosa (Amano AY-30) has good transesterification activity and can be used for biodiesel production. In this study, polyvinylidene fluoride (PVDF) membrane was grafted with 1,4-diaminobutane and activated by glutaraldehyde for C. rugosa lipase immobilization. After immobilization, the biocatalytic membrane was used for producing biodiesel from soybean oil and methanol via transesterification. Response Surface Methodology (RSM) in combination with a 5-level-5-factor central composite rotatable design (CCRD) was employed to evaluate the effects of reaction time, reaction temperature, enzyme amount, substrate molar ratio and water content on the yield of soybean oil methyl ester. By ridge max analysis, the predicted and experimental yields under the optimum synthesis conditions were 97% and 95%, respectively. The lipase-immobilized PVDF membrane showed good reuse ability for biodiesel production, enabling operation for at least 165 h during five reuses of the batch, without significant loss of activity.


Ultrasonics Sonochemistry | 2015

Ultrasound-assisted (R)-phenylephrine whole-cell bioconversion by S. marcescens N10612

Chi-Zong Zang; Shu-Chen Kan; Chiung-Wen Yeh; Chia-Chi Lin; Chwen-Jen Shieh; Yung-Chuan Liu

The strain Serratia marcescens N10612 is used to perform the bioconversion of 1-(3-hydroxyphenyl)-2-(methyamino)-ethanone (HPMAE) to (R)-phenylephrine ((R)-PE), which is an ephedrine drug substitute. The use of an ultrasound approach is found to improve the efficiency of the (R)-PE bioconversion. The optimization of the (R)-PE bioconversion is carried out by means of statistical experiment design. The optimal conditions obtained are 1.0mM HPMAE, 18.68 g/L glucose and ultrasound power of 120 W, where the predicted specific rate of the (R)-PE bioconversion is 31.46 ± 2.22 (ìmol/h/g-cells) and the experimental specific rate is 33.27 ± 1.46 (ìmol/h/g-cells), which is 3-fold higher than for the operation under ultrasound power of 200 W (11.11 ìmol/h/g-cells) and 4.3-fold higher than for the shaking operation (7.69 ìmol/h/g-cells). The kinetics study of the bioconversion also shows that under the ultrasound operation, the optimal rate (Vmax) of the (R)-PE bioconversion increases from 7.69 to 11.11 (μmol/h/g-cells) and the substrate inhibition constant (KSi) increases from 1.063 mM for the shaking operation to 1.490 mM for ultrasound operation.


Enzyme and Microbial Technology | 2016

Enhanced bioconversion rate and released substrate inhibition in (R)-phenylephrine whole-cell bioconversion via partial acetone treatment

Shu-Chen Kan; Chi-Zong Zang; Chiung-Wen Yeh; Wei-Feng Chang; Chia-Chi Lin; Tzu-Hsiang Hung; Chwen-Jen Shieh; Yung-Chuan Liu

An approach was developed to enhance the efficiency for the bioconversion of 1-(3-hydroxyphenyl)-2-(methyamino)-ethanone to (R)-phenylephrine. The strain Serratia marcescens N10612, giving the benefit of 99% enantiomeric excess in (R)-PE conversion, was used. The fermentation was devised to harvest cells with high hydrophobic prodigiosin content inside the cells. Then, the partial acetone extraction was applied to remove prodigiosin from the cells. The treatment was found to increase the cells conversion rate without loss of the cells NADPH redox system. When using 50% (v/v) acetone for 5min, the processed cells can give a specific conversion rate of 16.03μmol/h/g-cells. As compared the treated cells with cells under the basal medium, the maximum reaction rate (Vmax) increased from 6.69 to 10.27 (μmol/h/g-cells), the dissociation constant (Km) decreased from 0.236 to 0.167mM and the substrate inhibition constant (KSi) increased from 0.073 to 1.521mM. The 20-fold increase in substrate inhibition constant referred to a great release from the substrate inhibition for the use of S. marcescens N10612 in the bioconversion, which would greatly benefit the bioconversion to be industrialized.


Journal of Bioscience and Bioengineering | 2013

Production of D-hydantoinase via surface display and self-cleavage system.

Chia-Chi Lin; Tzu-Tsen Liu; Shu-Chen Kan; Chi-Zong Zang; Chiung-Wen Yeh; Jiun-Yan Wu; Jiann-Hwa Chen; Chwen-Jen Shieh; Yung-Chuan Liu

In this study, the cell surface expression system was the first time used to directly produce extracellular enzyme. In the plasmid construction, the truncated ice nucleation protein (INP) was fused with intein (INT) and target protein, D-hydantoinase (DHTase), to form the INP-INT-DHTase gene. The plasmid containing this gene was transformed into Escherichia coli ER2566 cells. The gene construct enables the expression of INP-INT-DHTase fusion protein, which might anchor on cell membrane surface. The induction conditions were studied and optimal conditions were as follows: E. coli ER2566 was incubated at 37°C and 200 rpm till OD₆₀₀ reached 0.6. Then, 0.05 mM IPTG was added and the induction was conducted at 15°C for 24 h. The cell was harvested and resuspended in the cleavage buffer (50 mM Tris-HCl buffer, pH 6). The cleavage reaction was carried out at 25°C, and 100 rpm for 24 h. The DHTase with an activity of 0.225 U/ml and a purity of 63.2% was obtained via centrifugation. This study demonstrated the feasibility of direct extracellular enzyme production using E. coli via only two steps of centrifugation.


Molecules | 2016

Antibacterial Peptide CecropinB2 Production via Various Host and Construct Systems

Wei-Shiang Lai; Shu-Chen Kan; Chia-Chi Lin; Chwen-Jen Shieh; Yung-Chuan Liu

Cecropin is a cationic antibacterial peptide composed of 35–39 residues. This peptide has been identified as possessing strong antibacterial activity and low toxicity against eukaryotic cells, and it has been claimed that some types of the cecropin family of peptides are capable of killing cancer cells. In this study, the host effect of cloning antibacterial peptide cecropinB2 was investigated. Three different host expression systems were chosen, i.e., Escherichia coli, Bacillus subtilis and Pichia pastoris. Two gene constructs, cecropinB2 (cecB2) and intein-cecropinB2 (INT-cecB2), were applied. Signal peptide and propeptide from Armigeres subalbatus were also attached to the gene construct. The results showed that the best host for cloning cecropinB2 was P. pastoris SMD1168 harboring the gene of pGAPzαC-prepro-cecB2 via Western blot confirmation. The cecropinB2 that was purified using immobilized-metal affinity chromatography resin showed strong antibacterial activity against the Gram-negative strains, including the multi-drug-resistant bacteria Acinetobacter baumannii.


Analytical Biochemistry | 2015

Development of an enzymatic chromatography strip with nicotinamide adenine dinucleotide-tetrazolium coupling reactions for quantitative l-lactate analysis

Shu-Chen Kan; Wei-Feng Chang; Min-Chi Lan; Chia-Chi Lin; Wei-Shiang Lai; Chwen-Jen Shieh; Kuang-Pin Hsiung; Yung-Chuan Liu

In this study, a dry assay of l-lactate via the enzymatic chromatographic test (ECT) was developed. An l-lactate dehydrogenase plus a nicotinamide adenine dinucleotide (NADH) regeneration reaction were applied simultaneously. Various tetrazolium salts were screened to reveal visible color intensities capable of determining the lactate concentrations in the sample. The optimal analysis conditions were as follows. The diaphorase (0.5 μl, 2(-6)U/μl) was immobilized in the test line of the ECT strip. Nitrotetrazolium blue chloride (5 μl, 12 mM), l-lactate dehydrogenase (1 μl, 0.25U/μl), and NAD(+) (2μl, 1.5×10(-5)M) were added into the mobile phase (100 μl) composed of 0.1% (w/w) Tween 20 in 10mM phosphate buffer (pH 9.0), and the process was left to run for 10 min. This detection had a linear range of 0.039 to 5mM with a detection limit of 0.047 mM. This quantitative analysis process for l-lactate was easy to operate with good stability and was proper for the point-of-care testing applications.


Journal of The Taiwan Institute of Chemical Engineers | 2014

Identification and enhanced production of prodigiosin isoform pigment from Serratia marcescens N10612

Chi-Zong Zang; Chiung-Wen Yeh; Wei-Feng Chang; Chia-Chi Lin; Shu-Chen Kan; Chwen-Jen Shieh; Yung-Chuan Liu


Journal of The Taiwan Institute of Chemical Engineers | 2014

Quantitative and morphologic analysis on exopolysaccharide and biomass production from a truffle endophytic fungus Hypocreales sp. NCHU01

Chiung-Wen Yeh; Chi-Zong Zang; Chia-Chi Lin; Shu-Chen Kan; Wei-Feng Chang; Chwen-Jen Shieh; Yung-Chuan Liu


Journal of The Taiwan Institute of Chemical Engineers | 2013

The use of mushroom hydrolysate from waste bag-log as the nitrogen source to mycelium biomass and exopolysaccharide production in Pleurotus eryngii cultivation

Hua-Bing Chen; Chih-I Chen; Mei-Jheng Chen; Chia-Chi Lin; Shu-Chen Kan; Chi-Zong Zang; Chiung-Wen Yeh; Chwen-Jen Shieh; Yung-Chuan Liu


Biochemical Engineering Journal | 2016

Analysis of exopolysaccharide production patterns of Cordyceps militaris under various light-emitting diodes

Chung-Hua Kho; Shu-Chen Kan; Chih-Yuan Chang; Heng-Yi Cheng; Chia-Chi Lin; Pin-Chiuan Chiou; Chwen-Jen Shieh; Yung-Chuan Liu

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Chwen-Jen Shieh

National Chung Hsing University

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Yung-Chuan Liu

National Chung Hsing University

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Chia-Chi Lin

National Chung Hsing University

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Chiung-Wen Yeh

National Chung Hsing University

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Chi-Zong Zang

National Chung Hsing University

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Wei-Feng Chang

National Chung Hsing University

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Jiun-Yan Wu

National Chung Hsing University

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Chih-I Chen

National Chung Hsing University

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Chi-Ming Chen

National Chung Hsing University

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Hua-Bing Chen

National Chung Hsing University

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