Shu-Juan Yang

A Comparison of the Effect of Work Stress on Burnout and Quality of Life Between Female Nurses and Female Doctors

ABSTRACT The objective of this study was to compare the effect of work stress on job burnout and quality of life between female nurses and doctors in China. The participants were 947 female nurses and 685 female doctors selected from Fujian provinces by using stratified cluster sampling method. The Chinese version of Short Form-36 Health Survey was used to measure quality of life; the Occupation Stress Inventory—Revised Edition was applied for occupational stress; and the Maslach Burnout Inventory—General Survey was used to assess job burnout. Occupational stress (indicated by different stressors) played an important role in job burnout and quality of life among female nurses and female doctors when taking into account other potential influencing factors simultaneously. These results show that it is important to adopt different preventive measures to prevent burnout and improve quality of life among the 2 populations according to the different stressors.

ADPRT Val762Ala and XRCC1 Arg194Trp polymorphisms and risk of gastric cancer in Sichuan of China.

OBJECTIVE Gastric cancer remains a major health problem in China. We hypothesized that XRCC1 Arg194Trp and ADPRT Val762Ala may be associated with risk. METHODS We designed a multicenter 1:1 matched case- control study of 307 pairs of gastric cancers and controls between October 2010 and August 2011. XRCC1 Arg194Trp and ADPRT Val762Ala were sequenced, and demographic data as well as lifestyle factors were collected using a self-designed questionnaire. RESULTS Individuals carrying XRCC1 Trp/Trp or Arg/Trp variant genotype had a significantly increased risk of gastric cancer (OR, 1.718; 95% CI, 1.190-2.479), while the OR for ADPRT Val762Ala variant genotype (Ala/Ala or Val/Ala) was 1.175 (95% CI, 0.796-1.737). No gene-gene or gene-environment interactions were found. In addition, family history of cancer and drinkers proportion were higher among cases than among controls (P<0.05). CONCLUSIONS XRCC1 194 Arg/Trp or Trp/Trp genotype, family history of cancer, and drinking are suspected risk factors of gastric cancer from our study. Our findings may offer insight into further similar large gene-environment and gene-gene studies in this region.

Polymorphisms of XRCC1 and ADPRT Genes and Risk of Noncardia Gastric Cancer in a Chinese Population: a Case-control Study

OBJECTIVE Gastric cancer (GC) is one of the most common malignancies and its mortality ranks third among all cancers in China. We previously noted that XRCC1 Arg194Trp was associated with GC risk in Western China in a study on XRCC1 Arg194Trp and ADPRT Val762Ala. We aimed to further explore the association of these polymorphisms with risk of the noncardia subtype. METHODS We enrolled 176 noncardia GC patients and 308 controls from four hospitals and a community between October 2010 and August 2011. Genotyping was performed in a 384-well plate format on the Sequenom MassARRAY platform. A self-designed questionnaire was utilized to collect epidemiological data from the subjects regarding demographic factors and potential risk factors. RESULTS Subjects were aged 56.8±11.8 (mean±standard deviation) and 57.6±11.1 years in the case and control groups, respectively. Individuals carrying the XRCC1 Trp/Trp or Arg/Trp variant genotype were at significantly increased risk of noncardia GC (adjusted OR, 1.48; 95% CI, 1.00-2.17), after adjustment for family history of cancer, drinking, and smoking. The increased risk of XRCC1 Arg194Trp variant genotype was more pronounced among subjects below 60 years old (adjusted OR, 1.78; 95% CI, 1.07-2.96), compared to older individuals. ADPRT Val762Ala variants (Ala/Ala or Val/Ala) were not associated with noncardia GC (adjusted OR, 1.03; 95% CI, 0.69-1.54). CONCLUSIONS Our study suggests that XRCC1 Arg194Trp is a genetic susceptibility factor for developing noncardia GC in Han Chinese in Western China. In particular, individuals with the XRCC1 Arg194Trp variant genotype are at increased risk for GC below 60 years old.

Interleukin-10 Gene Promoter Polymorphisms and Risk of Gastric Cancer in a Chinese Population: Single Nucleotide and Haplotype Analyses

OBJECTIVES Interleukin (IL) -10 is a potent cytokine with a dual ability to immunosuppress or immunostimulate. We aimed to explore the association of IL10 promoter polymorphisms with risk of gastric cancer (GC) in a Han population in Southwestern China. METHODS We enrolled 308 pairs of GC and control subjects from four hospitals and a community between October 2010 and August 2011 in a 1:1 matched case-control design. Demographic information was collected using a designed questionnaire. IL10-592 A>C and IL10-1082 A>G polymorphisms were determined by Sequenom MassARRAY analysis. RESULTS Patients with GC reported statistically higher proportions of family history of cancer (29.9% versus 10.7%, P<0.01) and alcohol drinking (54.6% versus 43.2%, P<0.01) than did controls. Similar results were observed in comparison between non-cardia GC patients and controls (P<0.01 and P=0.03). Variant genotypes of IL10-592 A>C and IL10-1082 A>G were not associated with overall GC risk (adjusted OR, 0.94, 95% CI, 0.66-1.33; adjusted OR, 1.00, 95% CI, 0.62-1.60). Sub-analysis showed that the IL10-592 AC/CC variant genotype was associated with decreased non-cardia GC risk (adjusted OR, 0.58; 95% CI, 0.36-0.95). No association was found between any of the IL10 haplotypes established from two polymorphisms and risk of non-cardia GC. CONCLUSIONS In conclusion, our data do not link the two SNPs of IL10-592 and IL10-1082 with overall GC risk. We demonstrate that IL10-592 polymorphism is associated with protective effect against non-cardia GC. Our findings may offer insight into risk associated with the development of GC in this region.

Interleukin-4 and -8 gene polymorphisms and risk of gastric cancer in a population in Southwestern China.

BACKGROUND Gastric carcinogenesis is a complicated process that involves environmental and genetic factors like interleukin-4 (IL-4) and IL-8. Single nucleotide polymorphisms in their genes are associated with changed levels of gene expression. Here, we investigated the association between IL4-590 C>T and IL8-251T>A and gastric cancer (GC) risk in Sichuan of Southwestern China. MATERIALS AND METHODS We surveyed the research subjects using a self-designed questionnaire with questions on demographic factors and putative risk factors. Approximately 2-5ml of whole blood was collected after field survey to analyze IL4-590 C>T and IL8-251T>A genotypes using MALDI-TOF MS. RESULTS Our study recruited 308 pairs of GC patients and controls, including 224 (72.7%) men and 84 (27.3%) women in each group. There were 99 cardia and 176 noncardia GC patients in the case group. The case and control groups had an average age of 57.7±10.6 (mean±SD) and 57.6±11.1 years. GC patients reported a significantly greater proportion of family history of cancer (29.9% vs 10.7%, p<0.01) and drinking (54.6% vs 43.2%, p<0.01) than did controls. Variant genotypes of IL-4-590 C>T and IL-8-251 T>A were not associated with overall GC risk (adjusted OR, 0.89; 95%CI, 0.61-1.28 for CT or CC vs TT; adjusted OR, 1.14; 95%CI, 0.86-1.79 for TA or AA vs TT). Stratification analysis of two SNPs for risk by subsites only found that variant IL-8-251 TA or AA genotype was associated with increased noncardia GC risk (adjusted OR, 2.58; 95%CI, 1.19-5.57). We did not observe interactions between the IL-8-251 T>A genotype and smoking (adjusted OR, 0.38; 95%CI, 0.08-1.79) or drinking (adjusted OR, 0.36; 95%CI, 0.08-1.65) for risk of noncardia GC. CONCLUSIONS Our data indicate no association between the two SNPs of IL-4-590 and IL-8-251 with overall GC risk, while the IL-8-251 TA or AA genotype conferred risk of cardia GC. Our findings contribute to the evidence body for risk of SNPs associated with the development of gastric cancer in this region.

Association of the IL-1B +3954 C/T polymorphism with the risk of gastric cancer in a population in Western China

With an estimate of 380 000 new cases each year, gastric cancer (GC) is one of the most frequently occurring cancers in China. Genes encoding proinflammatory and anti-inflammatory cytokines are good candidates for the study of susceptibility to GC. We tested the hypothesis that the polymorphisms of interleukin 1B (IL-1B) and IL-1RN contribute toward host susceptibility to GC. In a matched case–control design, we enrolled 308 pairs of GC and control participants between October 2010 and August 2011. We sequenced IL-1B +3954 C/T, IL-1RN −9876 G/A, −9739 A/G, and IL-1RN −9091 A/C using MALDI-TOF MS and collected demographic data as well as lifestyle factors using a questionnaire. GC patients reported statistically significantly greater proportions with family history of cancer (29.9 vs. 10.7%, P<0.01) and alcohol drinking (54.5 vs. 43.2%, P<0.01) than the controls. The proportion of irregular eaters was statistically higher among the patients than among the controls (66.7 vs. 24.4%, P<0.01). The IL-1B +3954 CT or the TT variant genotype was statistically significantly associated with a risk of GC [adjusted odds ratio (OR), 2.94; 95% confidence interval (CI), 1.06–8.15], whereas variants of IL-1RN −9876 G/A, IL-1RN −9739 A/G, and IL-1RN −9091 A/C were not associated (adjusted OR, 1.29, 95% CI, 0.77–2.16; adjusted OR, 1.25, 95% CI, 0.75–2.07; adjusted OR, 1.09, 95% CI, 0.71–1.67, respectively). Haplotypes established from the three polymorphisms of IL-1RN were not associated with a risk of GC. The IL-1B +3954 C/T polymorphism is associated with a risk of GC in our study. Lifestyle and environmental factors such as drinking, eating irregularly, and family history of cancer increase the risk.

Methylenetetrahydrofolate reductase genetic polymorphisms and esophageal squamous cell carcinoma susceptibility: a meta-analysis of case-control studies.

BACKGROUND Genetic factors and environmental factors play a role in pathogenesis of esophageal squamous cell carcinoma (ESCC). Previous studies regarding the association of folate intake and Methylenetetrahydrofolate reductase C677T polymorphism with ESCC was conflicting. We conducted a meta-analysis to investigate the association of MTHFR C677T and folate intake with esophageal cancer risk. METHODS MEDLINE, EMBASE and the Chinese Biomedical Database were searched in our study. The quality of studies were evaluated by predefined scale, and The association of polymorphisms of MTHFR C677T and folate intake and ESCC risk was estimated by Odds ratio (ORs) with 95% confidence intervals (CIs). RESULTS 19 studies (4239 cases and 5575 controls) were included for meta-analysis. A significant association was seen between individuals with MTHFR 677 CT [OR(95%)=1.47(1.32-1.63)] and TT [OR(95%)=1.69(1.49-1.91)] genotypes and ESCC risk (p<0.05). Low intake of folate had significantly higher risk of esophageal cancer among individuals with CT/TT genotype [OR(95%)=1.65(1.1-2.49)], while high intake of folate did not find significant high risk of esophageal cancer among individuals with CT/TT genotype [OR(95%)=1.64 (0.82-3.26)]. CONCLUSIONS Our meta-analysis indicated the folate intake and MTHFR 677CT/TT are associated with the risk of ESCC, and folate showed a significant interaction with polymorphism of MTHFR C677T.

The mediating and moderating role of personal strain and coping resource in the relationship between work stressor and quality of life among Chinese nurses

ObjectiveTo determine whether personal strain and coping resources act as either mediator or moderator or both in the relationship between work stressor and quality of life among Chinese nurses.MethodsA total of 1,012 nurses were selected from eight hospitals located in two provinces in China. Quality of life was measured with the Chinese version of the Short Form-36 Health Survey; work stressor, personal strain, and coping resources were evaluated using the Occupation Stress Inventory-Revised Edition. The hierarchical multiple regression procedure and Baron and Kenny’s model of mediation were applied to test for moderation and mediation, respectively. A structural equation model was fit to assess the interrelationships among these variables.ResultsWork stressor was closely associated with quality of life, which was mediated and moderated by personal strain and coping resources. Personal strain also acted both as moderator and mediator in the relationship between coping resources and quality of life. The relationships were verified in the structural equation model. The greatest absolute value of the standardized total effects was seen in personal strain (0.817), followed by work stressor (0.634) and coping resources (0.488).ConclusionsPersonal strain and coping resources have both mediating and moderating effects on the relationship between work stress and quality of life in a sample of Chinese nurses. An effective intervention strategy is needed to reduce work stress and ensure better quality of life.

Relationship of IL-17A and IL-17F genetic variations to cervical cancer risk: a meta-analysis

AIM We performed a meta-analysis to determine a more precise relationship of IL-17A and IL-17F polymorphisms with cervical cancer risk. MATERIALS & METHODS PubMed, CNKI and Wan Fang databases were searched for studies on these associations using STATA version 10.0 software. RESULTS Five studies were included. The AG and AA genotypes and A allele of IL-17A rs2275913 were correlated with an elevated risk of cervical cancer. The TT genotype and T allele of IL-17A rs3748067 and the CC genotype and C allele of IL-17F rs763780 carried a moderate risk of cervical cancer, when compared with the wild-type genotype. CONCLUSION IL-17A and IL-17F polymorphisms therefore have the potential to act as predictive biomarkers for cervical cancer risk.

Investigation on the association of occupational stress with risk of polycystic ovary syndrome and mediating effects of HOMA-IR

Abstract We aimed to evaluate the association between occupational stress and PCOS risk in a Chinese population and whether insulin resistance mediates the association. A total of 366 patients with PCOS and 325 controls were included in this study. Three logistic regression analyzes were applied in statistical analysis. In the first logistic regression analysis, the occupational stress significantly influenced development of PCOS (cumulative R2 = 0.737). In model 2, the environmental factors cumulatively accounted for 4.2% of the variance in PCOS risk. In model 3, which contained HOMA-IR, the R2 of HOMA-IR to PCOS risk was as high as 0.41, but the R2 of occupational stress reduced to 0.22. HOMA-IR became the main risk factor for PCOS. SEM model showed that ORQ, PSQ and PRQ had a direct and indirect effect on PCOS, and the indirect effect was through HOMA-IR. Occupational stress has a direct and indirect relationship with PCOS, which is mediated by HOMA-IR. 摘要 本研究旨在评估职业压力与PCOS患病风险在中国人群中的相关性, 以及胰岛素抵抗是否介导该相关性的形成。共纳入PCOS患者336名和对照组325名女性参与研究。统计分析采用三种Logistic回归分析。第一种Logistic回归模型表明, 职业压力对PCOS的发展有显著影响（累积R2=0.737）。Logistic回归模型2分析表明, 在PCOS风险中, 环境因素累计占4.2%。Logistic回归模型模型3中加入HOMA-IR作为变量, 结果显示, PCOS发生风险与HOMA-IR的相关性R2高达0.41, 但与职业压力的相关性R2则降至0.22。HOMA-IR成为PCOS发生相关的主要因素。SEM结果显示, ORQ, PSQ和PRQ结果对PCOS具有直接或间接作用, 且间接作用是由HOMA-IR介导。职业紧张与PCOS患病风险有直接和间接的相关性, 且该相关性受HOMA-IR影响。