Shuai Huang

Pyrrolizidine alkaloids from Liparis nervosa with inhibitory activities against LPS-induced NO production in RAW264.7 macrophages.

Six pyrrolizidine alkaloids were isolated from the whole herb of Liparis nervosa together with two previously known ones. Their structures were elucidated by extensive spectroscopic analyses and chemical reactions. The cytotoxicity of the isolates was evaluated against A549, HepG2, and MCF-7 human cancer cell lines; however, no significant growth inhibition was observed. All compounds were evaluated for the inhibition of LPS-induced nitric oxide (NO) production in RAW264.7 macrophages, and most significantly inhibited NO production with IC50 values in the range of 2.16-38.25 μM.

Five new nervogenic acid derivatives from Liparis nervosa

Ten new nervogenic acid derivatives (1-4, 6-11) and one known compound (5) have been isolated from Liparis nervosa. Their structures were determined using extensive spectroscopic analysis, including 1D and 2D NMR experiments. Compounds 3, 4, 9, 10, and 11 were evaluated for their cytotoxicity against A549, H460, Hela, MCF-7, Caco2, and HepG2 human cancer cell lines.

A new C20-diterpenoid alkaloid from Aconitum soongaricum var. pubescens.

Abstract A new denudatine-type C20-diterpenoid alkaloid, pubesine (1), along with seven known diterpenoid alkaloids, altaconitine (2), 14-benzoylaconine (3), spicatine A (4), 14-benzoylaconine-8-palmitate (5), 14-O-acetylsenbusine A (6), senbusine A (7) and 14-acetylneoline (8) were isolated from the whole plant of Aconitum soongaricum var. pubescens. Their structures were elucidated by means of extensive spectroscopic analyses (NMR and HR-ESI-MS) and comparison with data reported in the literature. All compounds were evaluated for their cytotoxicity against H460, MCF-7 and Hep G2 human cancer cell lines.

Diterpenoid Alkaloids from Two Aconitum Species with Antifeedant Activity against Spodoptera exigua

Twenty-five diterpenoid alkaloids were isolated from the roots of two Aconitum species. The structures of seven new C19-diterpenoid alkaloids, apetaldines A-G (1-7), and 10 known alkaloids (8-17) from Aconitum apetalum and eight known alkaloids (18-25) from Aconitum franchetii var. villosulum were elucidated via HRESIMS, IR, and NMR data. Alkaloids 1-10, 15, 16, and 18-25 were screened for their antifeedant activity. Among the compounds tested, chasmanthinine (19) showed highly potent antifeedant activity with an effective concentration for 50% feeding reduction (EC50) at 0.07 mg/cm2. The antifeedant structure-activity relationship of the diterpenoid alkaloids is also discussed.

A novel steroidal alkaloid from Fritillaria shuchengensis

A novel steroidal alkaloid, suchengbeisine (1), along with two known steroidal alkaloids, N-oxide of verticinone (2) and zhebeininoside (3), were isolated from the bulbs of Fritillaria shuchengensis S. C. Chen et S. F. Yin. The structures of these compounds were elucidated by intensive spectroscopic methods, including NMR, IR, CD and MS.

Identification of a new flavone glycoside from Codonopsis nervosa

A new flavone glycoside, luteolin 7-O-[(6″′-caffeoyl)-β-D-glucopyranosyl-(1 → 6)]-β-D-glucopyranoside (1), was isolated from Codonopsis nervosa, along with three other known compounds, luteolin 7-O-β-D-glucopyranoside (2), luteolin 7-O-gentiobioside (3), and tangshenoside VI (4). Their structures were determined on the basis of 1D and 2D NMR, IR, and HR-ESI-MS.

C18-Diterpenoid alkaloids from Delphinium anthriscifolium var. majus

Abstract Five new C18-diterpenoid alkaloids, anthriscifoltines C-G (1–5), along with four known diterpenoid alkaloids anthriscifolcines C-F (6–9), were isolated from the extract of Delphinium anthriscifolium var. majus. Their structures were elucidated by extensive spectroscopic analyses (including 1D-, 2D-NMR, and HR-ESI-MS). Compounds 1–5 were also evaluated for their cytotoxic activity against MCF-7, HepG2, and H460 human cancer cell lines.

Eremophilane derivatives from Senecio dianthus

From the aerial parts of Senecio dianthus, five eremophilane glucosides (1, 2, 4–6) and one new eremophilenolide (7) were isolated, together with sixteen known compounds (3, 8–22). Their structures and relative configurations were elucidated on the basis of extensive spectroscopic analysis, including HR-ESI-MS, X-ray, CD, 1D- and 2D-NMR experiments.

Diterpenoid Alkaloids from Delphinium anthriscifolium var. majus

Extensive phytochemical investigation on the whole herbs of Delphinium anthriscifolium var. majus led to the identification of fourteen diterpenoid alkaloids, including three new C20–diterpenoid alkaloids (anthriscifolsines A–C, 1–3), six new C19–diterpenoid alkaloids (anthriscifolrines A–F, 4–9), and five know compounds (10–14). Among them, anthriscifolsine A represents a novel C20–diterpenoid alkaloid characterized by a seco C–ring. The structures of the isolated compounds were elucidated by extensive spectroscopic methods, including HR-ESI–MS, X–ray, and 1D and 2D NMR experiments. Bioactivity of compounds 3–6 was evaluated for their cytotoxicity against the MCF–7, HepG2 and H460 cancer cell lines.

Five new C19 -diterpenoid alkaloids from Delphinium tianshanicum W. T. Wang.

Five new diterpenoid alkaloids, tianshanitines A‐E (1 – 5), along with ten known compounds (6 – 15), were isolated from the EtOH extracts of the whole plant of Delphinium tianshanicum W.T.Wang. Their structures were determined based on extensive spectroscopic analyses, including 1D‐ and 2D‐NMR, HR‐ESI‐MS, and the structure of tianshanitine C (3) was confirmed by X‐ray diffraction analysis. Tianshanitine A (1) is the first example of natural diterpenoid alkaloid containing a benzoyl group at C(1) position. Tianshanitine B (2) is a rare natural diterpenoid alkaloid bearing a OH group at C(16) position. Compounds 1 – 5, 6, 8, 10, 12 and 14 were evaluated for cytotoxicity against HCT116, MCF‐7 and HepG2 human cancer cell lines.