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Featured researches published by Shuhua Zhao.


The International Journal of Developmental Biology | 2010

Differential expression of the Brunol/CELF family genes during Xenopus laevis early development

Jingyang Wu; Chaocui Li; Shuhua Zhao; Bingyu Mao

The BRUNOL/CELF family of RNA-binding proteins plays important roles in post-transcriptional regulation and has been implicated in several developmental processes. In this study, we describe the cloning and expression patterns of five Brunol genes in Xenopus laevis. Among them, only Brunol2 is maternally expressed and the zygotic expression of the other four Brunol genes starts at different developmental stages. During Xenopus development, Brunol1, 4-5 are exclusively expressed in the nervous system including domains in the brain, spinal cord, optic and otic vesicles. Brunol2 and 3 are expressed in both the somatic mesoderm and the nervous system. Brunol2 is also extensively expressed in the lens. In transfected Hela cells, BRUNOL1, 2 and 3 proteins are localized in both the cytoplasm and the nucleus, while BRUNOL4 and 5 are only present in the cytoplasm, indicating their different functions.


Development Genes and Evolution | 2007

Cloning and developmental expression of the Xenopus Nkx6 genes.

Shuhua Zhao; Huifeng Jiang; Wen Wang; Bingyu Mao

The evolutionarily conserved Nkx6 family transcription factors play important roles in the patterning of the central nervous system (CNS) and pancreas in vertebrates. In this study, we describe the cloning and expression patterns of the three Nkx6 family genes in Xenopus laevis. Like their mouse and chicken homologues, Xenopus Nkx6 family genes are mainly expressed in the CNS and anterior endodermal tissues during embryonic development. Nkx6.1 and Nkx6.2 share overlapping expression domains in the ventral neural tube at neurula stages and later in the ventral part of developing hindbrain and spinal cord. Nkx6.3 is detected in the non-neural ectoderm from cleavage to early neurula stages and in the caudal hindbrain and the mandibular arch at tail bud stages. In the endoderm, Nkx6.2 is expressed in the hypochord at tail bud stages. At tadpole stages, the three Nkx6 genes are differentially expressed in the anterior endoderm derivatives, including the pancreas, stomach, esophagus, and lung.


Biochemical and Biophysical Research Communications | 2009

Islet-1 is required for ventral neuron survival in Xenopus

Yu Shi; Shuhua Zhao; Jiejing Li; Bingyu Mao

Islet-1 is a LIM domain transcription factor involved in several processes of embryonic development. Xenopus Islet-1 (Xisl-1) has been shown to be crucial for proper heart development. Here we show that Xisl-1 and Xisl-2 are differentially expressed in the nervous system in Xenopus embryos. Knock-down of Xisl-1 by specific morpholino leads to severe developmental defects, including eye and heart failure. Staining with the neuronal markers N-tubulin and Xisl-1 itself reveals that the motor neurons and a group of ventral interneurons are lost in the Xisl-1 morphants. Terminal dUTP nick-end labeling (TUNEL) analysis shows that Xisl-1 morpholino injection induces extensive apoptosis in the ventral neural plate, which can be largely inhibited by the apoptosis inhibitor M50054. We also find that over-expression of Xisl-1 is able to promote cell proliferation and induce Xstat3 expression in the injected side, suggesting a potential role for Xisl-1 in the regulation of cell proliferation in co-operation with the Jak-Stat pathway.


Biochemical and Biophysical Research Communications | 2011

Xenopus Dbx2 is involved in primary neurogenesis and early neural plate patterning

Pengcheng Ma; Shuhua Zhao; Wanli Zeng; Qiutan Yang; Chaocui Li; Xiaoyan Lv; Qin Zhou; Bingyu Mao

The evolutionarily conserved Dbx homeodomain-containing proteins play important roles in the development of vertebrate central nervous system. In mouse, Dbx and Nkx6 have been suggested to be cross-repressive partners involved in the patterning of ventral neural tube. Here, we have isolated Xenopus Dbx2 and studied its developmental expression and function during neural development. Like XDbx1, from mid-neurula stage on, XDbx2 is expressed in stripes between the primary motoneurons and interneurons. At the tailbud stages, it is detected in the middle region of the neural tube. XDbx2 acts as a transcriptional repressor in vitro and over-expression of XDbx2 inhibits primary neurogenesis in Xenopus embryos. Over-expression of XDbx genes represses the expression of XNkx6.2 and vise versa. Knockdown of either XDbx1, XDbx2 or both by specific morpholinos induces lateral expansion of XNkx6.2 expression domains. These data reveal conserved roles for Dbx in primary neurogenesis and dorsoventral neural patterning in Xenopus.


Development Genes and Evolution | 2013

Xenopus Nkx6.1 and Nkx6.2 are required for mid–hindbrain boundary development

Pengcheng Ma; Yingjie Xia; Li Ma; Shuhua Zhao; Bingyu Mao

The mid-hindbrain boundary (MHB) organizer is among the best studied local organizers that patterns the midbrain and cerebellum in vertebrates and is established by a regulatory network of several transcription factors and signals, including Wnt signaling. In the present study, we found that Xenopus Nkx6.1 and Nkx6.2, two transcription factors of the Nkx homeobox family, are required for MHB formation. In Xenopus embryos, nkx6.1 and nkx6.2 are expressed in the MHB, and knockdown of either nkx6.1 or nkx6.2 by specific morpholinos disrupts the MHB expression of en2 as well as wnt1, a key regulator for vertebrate MHB formation. In the nkx6.1/nkx6.2 morphants, co-injection of wnt1 or dngsk3β mRNA, which activates Wnt signaling, rescues the expression of en2. Nkx6.1 and Nkx6.2 activate canonical Wnt signaling in reporter assays in both cultured mammalian cells and Xenopus embryos. An Nkx6.2 deletion construct, which inhibits the ability of wild type Nkx6.2 to activate Wnt signaling, also reduces the MHB expression of en2 in Xenopus embryos. These results suggest that Nkx6.1 and Nkx6.2 are involved in MHB formation in Xenopus embryos, likely by modulating wnt1 expression.


FEBS Letters | 2016

Trip12 is an E3 ubiquitin ligase for USP7/HAUSP involved in the DNA damage response

Xiaoliang Liu; Xiangcai Yang; Yongxin Li; Shuhua Zhao; Chaocui Li; Pengcheng Ma; Bingyu Mao

The deubiquitinating enzyme, USP7/HAUSP (herpesvirus‐associated ubiquitin‐specific protease), is a key regulator of the tumor suppressor p53 and plays a major role in regulating genome stability. Here, we report that the protein stability of USP7 is regulated by the ubiquitin–proteasome pathway. We identified the thyroid hormone receptor interactor 12 (Trip12) as a ubiquitin E3 ligase for USP7. We also found that Trip12 affects USP7‐mediated stabilization of p53 and the checkpoint proteins 53BP1 and Chk1. Knockdown of Trip12 leads to an increased cell population in G1 phase, mimicking USP7 overexpression. In contrast, Trip12 overexpression increased the number of cells in intra‐S‐phase, phenocopying the USP7 knockdown phenotype. Therefore, our data reveal an important modulatory role for Trip12 in the USP7‐dependent DNA damage response.


Physical Review B | 2006

Intrinsic tunneling spectroscopy of Bi2Sr2CaCu2O8+delta: The junction-size dependence of self-heating

X. B. Zhu; Yueguang Wei; Shuhua Zhao; G H Chen; H. Yang; Az Jin; C.Z. Gu


Zoological Research | 2013

Involvement of XZFP36L1, an RNA-binding protein, in Xenopus neural development

Yingjie Xia; Shuhua Zhao; Bingyu Mao


Zoological Research | 2010

Potential Regulatory Role of the Conversed Elements in Sox23'Untranslated Region

Li Ma; Qing-hua Kong; Shuhua Zhao; Yun-hu Wei; Bingyu Mao


Zoological Research | 2007

Temporal and Spatial Expression Patterns of Sox 1 Gene in Xenopus laevis Embryo

Li Ma; Shuhua Zhao; Qing-hua Kong; Bingyu Mao

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Bingyu Mao

Kunming Institute of Zoology

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G H Chen

Chinese Academy of Sciences

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Chaocui Li

Kunming Institute of Zoology

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Li Ma

Chinese Academy of Sciences

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Pengcheng Ma

Chinese Academy of Sciences

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Yueguang Wei

Chinese Academy of Sciences

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X. B. Zhu

Chinese Academy of Sciences

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Yingjie Xia

Chinese Academy of Sciences

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C.Z. Gu

Chinese Academy of Sciences

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