Sibel Gunes

Hematoprotective effect of seleno-L-methionine on cyclophosphamide toxicity in rats

Cyclophosphamide (CP) is a widely used antineoplastic drug that causes toxicity in the normal cell due to its metabolites. The major drawback of this drug is an undesirable myelosuppression. Selenium (Se) is a potent nutritional antioxidant that carries out biological effects by its incorporation into selenoproteins, such as glutathione peroxidase (GPx). The possible protective effects of seleno-l-methionine (SLM) against CP-related toxicity of blood cells and bone marrow of rats were investigated in this study. Intraperitoneal (i.p) administration of 50, 100, or 150 mg/kg of CP caused, in a dose-dependent manner, reductions in the number of leukocytes (78, 89, and 92%, respectively), thrombocytes (22, 33, and 52%, respectively), and bone marrow–nucleated cells (72, 90, and 94%, respectively). The groups that had CP treatment alone were killed 3 days after the CP injection. For the groups having CP+SLM, SLM (0.4 or 0.8 mg/kg i.p) administration was started 3 days earlier than the CP administration and continued to the end of the experiment (6 days). On day 4, the animals were weighed again, relative doses of CP were estimated, and CP+SLM was administered together. On day 7, blood samples were collected and bone marrow of animals were resected under anesthesia. The results indicated that treatment of rats within a select dose range of SLM could reduce CP-induced toxicity on blood cells and bone marrow.

Carvacrol attenuates cyclophosphamide-induced oxidative stress in rat kidney

Cyclophosphamide (CP) is an antineoplastic drug that induces kidney damage via producing oxidative stress. Carvacrol (CAR) has antioxidative effect and we postulated that it can be protective against CP-induced nephrotoxicity. Six groups (n = 7) of rats (control, 100 mg/kg CP, CP+5 mg/kg CAR, CP+10 mg/kg CAR, 5 mg/kg CAR, and 10 mg/kg CAR) were injected intraperitoneally. Serum malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), creatinine (CRE), total antioxidant capacity (TAC), and total oxidant state (TOS) were measured, and oxidative stress indexes (OSI) were calculated. Kidneys were also analyzed histologically. In CP-alone group MDA, CRE, TOS, and OSI levels increased whereas GSH, SOD, CAT, and TAC levels decreased compared with control group. In CP plus CAR groups, MDA, TOS, and OSI levels decreased whereas GSH, SOD, CAT, and TAC levels increased compared with CP-alone group. However, CRE levels were similar in CP-alone and CP+5 CAR group whereas decreased in CP+10 CAR group. CP+10 CAR group was significantly different in all parameters (except TAC) from CP+5 CAR group. Kidney microscopy was showed lower tissue damage in CP plus CAR groups. In conclusion, 10 mg/kg CAR is more effective than 5 mg/kg CAR in prevention of CP-induced oxidative damage on kidney.

Cell and Tissue Culture: The Base of Biotechnology

Abstract A cell culture is basically keeping the cells taken out of a source in an artificial environment alive. In cell culture systems it is aimed that the cells that are in three-dimensional environment in tissues to be cultured and examined by providing appropriate circumstances under in vitro conditions. There are very common areas of use in cell cultures. It is possible to encounter with these kinds of studies in nearly all the fields. The areas in which the cell culture is used can be counted as the production of monoclonal antibody, vaccine, enzyme, hormone, interleukin, and some growth factors. Also cell culture studies serve too many fields such as toxicology studies, development of new drugs, and cell or stem cell therapy methods. Besides, nowadays especially in parallel with the improvements that are experienced in stem cell and the field of genetics, cell culture studies have come to an important place in biotechnology, cell therapy, and regenerative medicine field. Scientists around the world make use of cell culture techniques on a daily basis. Whether they happen to be working with primary cell cultures, secondary cultures, or cell lines, they all face many of the same problems: slow growth, spontaneous differentiation, evaporation, contamination, and a host of other issues that require troubleshooting. Consequently, cell culture studies need special working conditions, special equipment, and experienced staff.