Sidney Anthone

Tubular Lesions Produced by Autoantibodies to Tubular Basement Membrane in Human Renal Allografts

In two patients with chronic glomerulonephritis who received renal allografts, transplant failure was associated with binding of transplantation antibodies to the graft and with glomerular and vascula

Sister chromatid exchange and chromosome abnormalities in uremic patients

Chronic renal failure heightens the risk of malignancy. We therefore examined lymphocytes from 44 uremic patients and 24 normal controls for chromosome abnormalities and sister chromatid exchange (SCE) rate. This is the first report of SCE in uremia. Uremia was found to increase structurally abnormal chromosomes and elevate the rate of SCE. These cytogenetic changes in uremia may play a role in the heightened risk of cancer.

Recurrence of Henoch-Schönlein purpura glomerulonephritis in transplanted kidneys.

An 8-year-old boy developed a skin rash, gastrointestinal symptoms, and acute glomerulonephritis with immunopathological findings characteristic of Henoch-Schönlein purpura. After renal transplantation the skin rash, gastrointestinal symptoms, and glomerulonephritis recurred. The similarity between the immunopathological changes found in the hosts kidneys and those observed in the graft are consistent with the interpretation of a transmission of Henoch-Schönlein purpura to the renal transplant.

Circulating immune complexes after kidney transplantation.

Sera from patients who had received renal allografts were studied for the presence of circulating immune complexes by using platelet aggregation technique combined with density gradient centrifugation. A simple and highly reproducible modification of the platelet aggregation technique, employing the use of relatively small amounts of blood from pretested donors as the source for platelets, is described. Immune complexes were detected in post-transplantation sera from 3 out of 16 patients. The development of a persistent immune complex state as a consequence of grafting was concluded in one patient.

Colloid Osmotic Pressure Fluctuations and the Disequilibrium Syndrome during Hemodialysis

Changes in colloid osmotic pressure (COP), osmolality, blood urea nitrogen, glucose, electrolytes and arterial blood gases were correlated with the symptoms that occurred during 46 dialyses in 10 stab

HETEROPHILE ANTIGEN IN SERA OF HUMAN RECIPIENTS OF RENAL ALLOGRAFTS

Abstract It was noted that many sera of patients with renal allograft produce distinct precipitation lines in gel diffusion tests with about 20% of infectious mononucleosis sera. The antibodies in infectious mononucleosis sera were of IgM isotope, but, interestingly, they could be removed by guinea pig kidney homogenate, which indicated that the reactions studied were of the Hanganutziu-Deicher rather than of the Paul-Bunnell type. This contention was strengthened by the fact that positive transplantation sera reacted also with standard serum with Hanganutziu-Deicher antibodies. Thus far, the presence of the antigen in the transplantation sera could not be related to the clinical status of the patients, however, the antigen was noted primarily in those sera that did not contain heterophile transplantation antibodies. It was proposed that the antigen detected in the transplantation sera was an altered tissue antigen released from the grafted organ. Besides, interactions between two serum samples from the same patient were noted in immunodiffusion tests. These reactions occurred very seldom and were unrelated to heterophile transplantation antigens or antibodies.

Physiologic Response Patterns to Occlusion of Clinically Significant Arteriovenous Fistulas

Seven patients with hemodynamically significant arteriovenous fistulas for dialysis angioaccess were studied by multivarient computer analysis of physiologic data derived from cardiogreen dye dilution curves and A-VO2 before and after acute occlusion of their fistulas and prior to clinical therapy. Three patterns of response were characterized. These patterns seemed to be related to the innate ventricular contractility status, the type of medications, the presence of autonomic neuropathy, and their interplay with alterations in preload and afterload with fistula occlusion. In one patient, the response pattern precluded banding or occlusion of the fistula. In the remainder, the physiolobic responses permitted treatment by banding or occlusion. It seems advisable to adequately evaluate the physiologic response patterns of patients with hemodynamically significant arteriovenous fistulas prior to definitive treatment.

Unusual Precipitins in Pathological Human Sera

In conducting double diffusion gel precipitation tests, unexpected, strong precipitation reactions were noticed between a serum originating from a renal graft recipient and many pathological human sera. The highest frequency of positive reactions were produced by sera of patients with infectious mononucleosis (83%), chronic Epstein-Barr virus infections (72%), rheumatoid arthritis (57%), lepromatous leprosy (57%), and AIDS (44%). The precipitin in these pathological sera was identified as an antibody of IgM variety and the precipitinogen in the transplantation serum was shown to be a thermostable component with beta-globulin mobility. No explanation of the nature of reactions observed can be given at present.

A simple technique of correcting complete gastric obstruction after gastric partitioning

The use of autosuture staplers in intraabdominal surgery has become an accepted procedure for simplifying gastrointestinal surgery. Described in this report is a simple method of correcting unrelenting gastric obstruction that may occur after gastric partitioning for morbid obesity by utilizing the autosuture model EEA stapler to reestablish gastric continuity.