Sidney J. Bennett

Use of radiolabeled antibodies to carcinoembryonic antigen for the detection and localization of diverse cancers by external photoscanning

To determine whether tumors containing carcinoembryonic antigen could be detected by administration of a radiolabeled, affinity-purified, goat lgG having 70 per cent immunoreactivity against carcinoembryonic antigen, 18 patients with a history of cancer of diverse histopathology received an average total dose of 1.0 mCi of 131l-labeled lgG. Total-body photoscans were performed with a gamma scintillation camera at various intervals after administration of the radioactive antibody. Ordinary photoscans proved difficult to interpret because of blood-pool background radioactivity, thus necessitating the computer subtraction of radioactive blood-pool agents from the antibodys 131l activity. Tumor location could be demonstrated at 48 hours after injection in almost all cases studied. The scans were negative in patients without demonstrable tumors or with tumors apparently devoid of carcinoembryonic antigen. Circulating antigen levels of up to 350 ng per milliliter did not prevent successful tumor imaging after injection of the radioantibody.

Carcinoembryonic Antigen and Bladder Carcinoma

The 24-hour urinary carcinoembryonic antigen determinations were performed on 61 patients with different stages of bladder carcinoma. Elevated titers were found in 81 per cent of the patients with active tumors and falsely positive studies were found in 7 per cent. High stage lesions were found to have high carcinoembryonic antigen levels. Plasma carcinoembryonic antigen determinations were elevated in only 45 per cent of the patients with active tumors but further study may be warranted in advanced bladder cancer cases. The 24-hour urinary carcinoembryonic antigen measurements yield the highest percentage elevations in bladder carcinoma and further investigation is required to better define its clinical application.

Adenocarcinoma of the small intestine arising in crohn's disease: Demonstration of a tumor-associated antigen in invasive and intraepithelial components

A segment of small intestine surgically removed from a man with intestinal obstruction was found to have coexisting regional enteritis and an invasive adenocarcinoma associated with an area of intraepithelial neoplasia. The cells of the adenocarcinoma and the intraepithelial neoplasia contained a tumor‐associated surface antigen capable of reacting with an antiserum prepared against a colonic carcinoma. The importance of thorough sampling of specimens of regional enteritis in accurate reporting of the incidence of carcinoma is stressed, as is the use of immunohistologic techniques as an adjunct to the morphologic diagnosis of preinvasive neoplasia.

Carcinoembryonic antigen in gynecologic patients: I. Correlation of plasma levels and tissue localization☆

Abstract Plasma levels of carcinoembryonic antigen (CEA) were measured in 36 patients with suspected gynecologic malignancies from whom histological tissues were available for antigenic localization. The latter was done utilizing the three-layer bridge immunoperoxidase technique for paraffin sections. The presence or absence of tissue CEA as determined by this method is correlated with the plasma levels of CEA in each patient.

Studies on tumor-associated antigen: TAA

Abstract A method for the preparation of tumor-associated antigen (TAA) is described. We have been able to demonstrate two antigens with common antigenic determinants that are separable by ion-exchange chromatography and electrophoresis. These antigens appear identical by Ouchterlony immuno-diffusion analysis and by Sephadex chromatography. The differences in electrophoretic mobility of these antigens appear related to a sialic acid moiety, although we have demonstrated that the antigenic specificity does not reside in this portion of the molecule. These antigens share at least one common determinant with a low-molecular-weight substance found in perchloric acid extracts of normal lung and colon. This and previous studies would suggest that this antigen is identical to carcinoembryonic antigen (CEA).