Silke Jacobs

Risk assessment of methylmercury in five European countries considering the national seafood consumption patterns

Although seafood is a nutritious protein source, due to marine environmental pollution, seafood may also be a source of contaminants. The results obtained within the FP7-ECsafeSEAFOOD-project show that among the range of studied environmental contaminants certainly methylmercury (MeHg) requires deeper investigation. This paper presents the results of a probabilistic risk assessment for MeHg based on: (1) primary concentration data, as well as secondary data from published papers, and (2) primary species-specific consumption data collected in five European countries (Belgium, Ireland, Italy, Portugal and Spain). The results indicated that in the southern European countries, larger subgroups of the population (up to 11% in Portugal) are potentially at risk for a MeHg exposure above the Tolerable Weekly Intake (TWI) value, while this risk is much lower in Ireland and Belgium. This research confirms the substantial contribution of tuna to MeHg exposure in each of the countries. Also hake, cod, sea bream, sea bass and octopus are identified as important contributors. From this study, it is concluded that a country-specific seafood consumption advice is needed. Policy makers may adopt the results of this study in order to develop consumer advices that optimise health benefits versus potential health risks by providing species-specific information.

Marine environmental contamination: public awareness, concern and perceived effectiveness in five European countries

Given the potential of Perceived Consumer Effectiveness (PCE) in shaping pro-environmental behavior, the relationships between PCE, awareness of causes of contaminants in the marine environment, and concern about marine environmental contamination were investigated using Structural Equation Modeling (SEM). PCE is the belief that an individual has in being able to make a difference when acting alone. A web-based survey was performed in one western European country (Belgium), one northern European country (Ireland) and three southern European countries (Italy, Portugal and Spain), resulting in a total sample size of 2824 participants. The analyses confirm that European citizens are concerned about marine environmental problems. Participants from the southern countries reported the highest concern. In addition, the study participants did not have a strong belief in themselves in being capable of making a difference in tackling marine environmental problems. However, a higher awareness, which was associated with a higher degree of concern, enhanced the belief that an individual can make a difference in tackling marine environmental problems, though only when a concrete action was proposed. Consequently, information campaigns focusing on pro-environmental behavior are recommended to raise public awareness about marine environmental problems and at the same time explicitly refer to concrete possible actions. The findings indicate that when only awareness and concern are raised without mentioning a concrete action, PCE might even decrease and render the communication effort ineffective.

Integrated risk index for seafood contaminants (IRISC): pilot study in five European countries

Consumption of seafood is one of the most relevant pathways of exposure to environmental pollutants present in food. The list of toxic compounds in seafood is very extensive, including heavy metals, polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs). In order to quantify the importance of the problem, tools to combine and simplify large data collections are mandatory for risk managers and decision-makers. In this study, the development of a prioritization setting focusing on chemical hazards taken up through seafood was aimed. For this purpose, the toxicity data of several chemicals was integrated with concentration and seafood consumption data, building an integrated risk index for seafood contaminants (IRISC) able to draw a map of risk for each chemical and family of chemicals. A pilot trial was performed on a sample of 74 pollutants, four seafood species and five European countries (Belgium, Ireland, Italy, Portugal and Spain). The preliminary results revealed that Portugal and Spain presented the highest IRISC, while Belgium was the region with the lowest IRISC. The contribution of each group of contaminants to the IRISC was very similar among countries, with heavy metals being the major contributor, followed by PCBs, PCDD/Fs and endocrine disrupting compounds. When the contribution of different seafood species to the Risk Indexes (RIs) was compared, the results elucidated the high input from sardines, showing the highest rates (54.9-76.1) in the five countries. The IRISC provides a friendly approach to the chemical risk scene in Europe, establishing normalized prioritization criteria considering toxicity and consumption as well as concentration of each chemical.

UV-filters and musk fragrances in seafood commercialized in Europe Union : occurrence, risk and exposure assessment

ABSTRACT In the framework of the FP7 ECsafeSeafood project, 62 seafood samples commercialized in Europe Union from several representative species – mackerel, tuna, salmon, seabream, cod, monkfish, crab, shrimp, octopus, perch and plaice – were analysed for residues of 21 personal care products (PCPs), including 11 UV‐filters (UV‐Fs) and 10 musk fragrances (musks). PCPs analysis were performed by Quick, Easy, Cheap, Effective Rugged, Safe (QuEChERS), combined with liquid‐liquid extraction (LLE) or dispersive solid‐phase extraction (dSPE), followed by gas chromatography‐tandem mass spectrometry (GC‐MS/MS). The results showed the presence in a wide range of samples of nine out of eleven UV‐Fs compounds analysed, namely 2‐ethylhexyl salicylate (EHS), 2‐ethylhexyl,4‐methoxycinnamate (EHMC), 4‐methylbenzylidenecamphor (4‐MBC), benzophenone‐1 (BP1), benzophenone‐3 (BP3), isoamyl‐4‐methoxycinnamate (IMC), 2,2′‐dihydroxy‐4,4′‐dimethoxybenzophenone (DHMB), homosalate (HS), and octocrylene (OC), whereas galaxolide (HHCB), galaxolide lactone (HHCB‐lactone), and tonalide (AHTN) were the most found musks. The potential risks to human health associated with the exposure to eight of the more prevalent PCPs – EHS, EHMC, 4‐MBC, BP1, BP3, IMC, HHCB, and AHTN ‐ through seafood consumption were assessed for consumers from five European countries (Belgium, Ireland, Italy, Portugal and Spain). Results showed that the human exposure to UV‐Fs and musks estimated from the concentration values found in seafood and the daily consumption of concerned seafood species, were far below toxicological reference values. HIGHLIGHTSLargest survey on UV‐Fs and musks in seafood commercialized in Europe.Farmed seabream showed the highest average concentration of UV‐Fs.Wild mackerel showed the highest average concentration of polycyclic musks.UV‐Fs and musks in raw samples were far below the estimated toxicological reference value.

Health risk/benefit information for consumers of fish and shellfish: FishChoice, a new online tool

It is well known that due to the content in omega-3 fatty acids, consumption of fish and shellfish is beneficial for human cardiovascular health. However, a number of recent studies have shown that fish consumption may be also a potential dietary source of exposure to various environmental pollutants with well-known potential adverse effects on human health. Moreover, there is still a lack of information regarding levels of emerging contaminants in fish and shellfish, in particular among consumers and stakeholders. Within the ECsafeSEAFOOD FP7 project, a wide variety of emerging contaminants including brominated flame retardants, pharmaceuticals, perfluoroalkyl substances, musk fragrances, polycyclic aromatic hydrocarbons, UV-filters and endocrine disruptors, as well as inorganic arsenic and methylmercury, were analyzed in fish and shellfish samples collected all over the European Union. These data, together with those regarding nutrient concentrations from different European food composition databases, were integrated into a new online tool, called FishChoice. In this paper, we report how FishChoice was designed and present its main improvements compared to previous tools or software programs, in terms of selected pollutants, number of species, and specific recommendations for an optimal consumption of fish and shellfish.

Occurrence of cyclic imines in European commercial seafood and consumers risk assessment

ABSTRACT Cyclic imines constitute a quite recently discovered group of marine biotoxins that act on neural receptors and that bioaccumulate in seafood. They are grouped together due to the imino group functioning as their common pharmacore, responsible for acute neurotoxicity in mice. Cyclic imines (CIs) have not been linked yet to human poisoning and are not regulated in the European Union (EU), although the European Food Safety Authority (EFSA) requires more data to perform conclusive risk assessment for consumers. Several commercial samples of bivalves including raw and processed samples from eight countries (Italy, Portugal, Slovenia, Spain, Ireland, Norway, The Netherlands and Denmark) were obtained over 2 years. Emerging cyclic imine concentrations in all the samples were analysed on a LC‐3200QTRAP and LC‐HRMS QExactive mass spectrometer. In shellfish, two CIs, pinnatoxin G (PnTX‐G) and 13‐desmethylspirolide C (SPX‐1) were found at low concentrations (0.1–12 &mgr;g/kg PnTX‐G and 26–66 &mgr;g/kg SPX‐1), while gymnodimines and pteriatoxins were not detected in commercial (raw and processed) samples. In summary, SPX‐1 (n: 47) and PnTX‐G (n: 96) were detected in 9.4% and 4.2% of the samples, respectively, at concentrations higher than the limit of quantification (LOQ), and in 7.3% and 31.2% of the samples at concentrations lower than the LOQ (25 &mgr;g/kg for SPX‐1 and 3 &mgr;g/kg for PnTX‐G), respectively. For the detected cyclic imines, the average exposure and the 95th percentile were calculated. The results obtained indicate that it is unlikely that a potential health risk exists through the seafood diet for CIs in the EU. However, further information about CIs is necessary in order to perform a conclusive risk assessment. HIGHLIGHTSFirst study of cyclic imines in commercial shellfish samples from Europe.First study including samples from eight European countries during two years.First report dealing risk assessment on pinnatoxins.Completes information regarding spirolides from the EFSA report on 2010.Increases database on levels of CIs in shellfish to perform a risk assessment.

Consumer response to health and environmental sustainability information regarding seafood consumption

ABSTRACT Seafood consumption has an impact on both consumers’ health and on the marine environment, making the integration of health and sustainability aspects in information and recommendation messages for consumers highly topical. This study presents the results of a consumer study in terms of the impact of exposure to a message about health and sustainability aspects of seafood on 986 participants from Belgium and Portugal. Possible drivers for behavioural change regarding seafood consumption frequency and sustainable seafood buying frequency are studied following exposure to the message. Initial behaviour emerges as the most important factor triggering a change in the intention to consume seafood twice per week and a change in the intention to buy sustainable seafood. A higher health benefit perception resulted in an increased intention to consume seafood twice per week. Attitude towards the message and the option to optimise consumers’ choice of seafood species favouring sustainability were significant determinants of change in the intention to buy sustainable seafood. Different stakeholders may take the results of this communication strategy into account and, consequently, contribute to a seafood supply and related communication that supports public health and the marine environment. HIGHLIGHTSFive segments were identified based on intentions towards healthy and sustainable seafood choice.Attitudes towards messages combining health and sustainability are generally favourable.Risk/benefit perception and perceived consumer effectiveness are affected by message exposure.Communication emphasizing the link between health and sustainability related to seafood is recommended.

The impact of a message containing health and ecology aspects related to seafood consumption

Introduction: In the last years and decades more and more new plants came to the market as food or old crops have been rediscovered. Based on the presence of secondary plant metabolites specific effects are often attributed to these plants and/or derived preparations. Depending on the amount and the accompanying substances in extracts and preparations these substances can also be toxic. In many cases, plants and plant preparations are not adequately tested for their safety before marketing. In the European Union, authorization procedures and health assessments are only required for foods that are considered novel foods or produced from genetically modified organisms. A working group of federal and state governments, the BfR has worked with, has drawn up a list of nearly 600 plants and plant parts, which should facilitate the assessment of these plants (or plant parts) and derived preparations by food inspection bodies and food industry. Objectives: 18 plants or parts of plants were selected for an evaluation because of their known pharmacological or psychotropic effects or due to possible health risks. Method / Design: The risk assessments of these plants or parts of plants, including for example goji berries and yohimbe bark, were performed using the “Guidance on Safety assessment of botanicals and botanical preparations intended for use as ingredients in food supplements” of the European Food Safety Authority (EFSA). Results: Nine of the 18 plants or parts of plants pose a risk to consumers and should not be used in food. Five plants or parts of plants might pose risks when used in food. For four plants or plant parts, no risks were seen. Conclusions: The health assessments are intended to be a first step in the process of harmonization at European level. The opinions are published in a booklet and accessible on the web(04/08/2019) Organic food for sustainable and healthy diets lessons from the nordic diet? Introduction: The New Nordic Diet (NND) was developed in 2004 by chefs and food professionals from the five Nordic countries. The goal for the NND was that it should be based on traditional regional food products but healthier than the traditional eating habits. The NND builds on four key principles: Nordic identity, health, gastronomic potential and sustainability.Objectives: Can the NND be used as a model for a sustainable diet in other geographical regions?Methods/design: The NND can be described by a few overall guidelines: 1) more calories from plant foods and fewer from meat; 2) more foods from the wild countryside and 3) more foods from sea and lakes. In many ways, the New NND is very similar to a Mediterranean diet but relies on rapeseed (canola) oil instead of olive oil and ramson instead of garlic. The diets differ in their types of produce due to regional differences in climate, soil and water.Results: The health effects and sustainability of the NND has been tested in a number of scientific studies, including the OPUS project (Optimal Well-Being, Development and Health for Danish Children through a Healthy New Nordic Diet) supported by the Nordea foundation (http://foodoflife.ku.dk/opus/english/nyheder/publikationer/) in which the NND was compared to the Average Danish Diet (ADD). The use of mostly local products and reduction of the meat intake were of both socioeconomic and environmental advantage. Including organic produce increased environmental impact of the NND.Conclusion: In line with the Mediterranean diet the NND is a predominantly plant-based diet, and although the two have not been directly compared, it would be fairly safe to assume that they are equally healthy. Overall, the NND is just a regional interpretation of the tenets of healthy eating. Basically the principles of the NND could be incorporated into any regional diet.Who are we eating with? There are always companions to relate to in a meal, both to those who are present and they who are on another place or even in a past history. The choice of food and beverag ...Objectives : The aim of the present work was to compare the effect of ALA, EPA and DHA on the development of adipose tissue and its metabolism during a high fat-high sucrose (HFHS) challenge.Ann Nutr Metab 2015; 67(suppl 1) 435 BMD (BL: -2.4±0.7, PI: -0.9±0.44), serum calcium (BL: 9.5±0.6, PI: 10.5±0.52) and vitamin D (BL: 17.63±4.9, PI: 42.72±8.9) was observed. Locomotor problems were reduced among 44.06% subjects in group B compared to 11.11% in group A. Post interventional BMD (t: -2.16, P≤0.05), serum calcium (t: -4.05, P≤0.001) and vitamin D (t: -4.20, P≤0.001) of group B was significantly higher compared to group A.Affiliation: (1) PhD Student in Food Science. Human Nutrition Unit. Department of Food Science. University of Parma. Italy; (2) Associate Professor. Department of Food Science. University of Parma. Parma. Italy; (3) Full Professor. Department of Food Science, University of Parma. Parma. Italy; (4) Post-Doc Research Assistant. Department of Agricultural, Forest and Food Science, University of Turin. Torino. Italy; (5) Assistant Professor. Department of Soil, Plant and Food Science. University of Bari Aldo Moro. Bari. Italy; (6) Post-Doc Research Assistant. Department of Pharmacy and Biotechnology, Alma Mater Studiorum University of Bologna. Bologna. Italy; (7) Full Professor. Department of Soil, Plant and Food Science. University of Bari Aldo Moro. Bari. Italy; (8) Nutritionist. Department of Food Science. University of Parma. Parma Italy.Effect on attention of a vegetable smoothie, rich in berries, fruitsand vegetables, served at the school mid-morning brake. ViktoriaOhlsson. Kristianstad UniversityPurpose:The purpose of this pilot ...Introduction: Since appetite control works differently in fluid and solid intake we wanted to analyse the energy contribution from those two types of energy sources.Objectives: The objective of thi ...Introduction: Infrequent fruit and vegetable (FV) intake is especially common among children with low parental educational level (PEL) and among boys.Objectives: Our aim was to examine whether a school-based intervention was efficient in increasing children’s FV intake especially among those whose FV intake is the lowest and which factors could explain the the group differences in the associations.Method / Design: In Finland 11-year-old (at baseline) children participated in the PRO GREENS intervention in winter 2009. In control schools were 424 and in intervention schools 386 children (response rate 77%). Children filled in validated food frequency questionnaire assessing FV intake (times/day) and a validated questionnaire about factors influencing FV intake (availability of FV, liking for FV, preferences, self-efficacy to eat FV, attitudes towards FV and knowledge of the recommendations) both at baseline May 2009 and follow-up May 2010. Parental educational level (low, middle, high) was reported by the parents. Associations were examined with linear regression and mediation analyses.Results: The intervention increased fruit intake among girls but not among boys. Intervention increased also children’s knowledge of the recommendations. Since knowledge had no impact on boys’ fruit intake, the increase in knowledge mediated only intervention’s effect on girls’ fruit intake. Intervention increased children’s fruit intake similarly in all PEL groups.Intervention increased vegetable intake only in the middle PEL group but no intervention effect was noted among children with low or high PEL. Knowledge, the only factor which mediated the intervention’s effect on children’s vegetable intake, could not explain PEL differences in the effectivity of the intervention.Conclusions: Increase in knowledge was not a sufficient prerequisite to increase FV intake among boys or the lowest PEL group. More in depth analyses are needed to find out which factors to target in interventions to reach an effect in the target groups.