Silvana Diverio

The effect of management practices on stress in farmed red deer (Cervus elaphus) and its modulation by long-acting neuroleptics: behavioural responses

Abstract Eighteen red deer hinds were used to study the behavioural responses over a period of 10 weeks to three management practices which were expected to cause increasing levels of stress. The practices were herding, herding and handling and herding, handling and a veterinary procedure. One of three groups of six animals received a long-acting neuroleptic (LAN—perphenzine enanthate and zuclopenthixol acetate) in Weeks 1, 5 and 9 of the experiment whilst the other groups were untreated. The LAN-treated and one of the untreated groups received the stressors in sequence and one undisturbed group acted as a control. The siting of the paddocks in which the three groups were rotated had a marked effect on behaviour: the further the deer were from the handling area, the less stress-related behaviour they exhibited. In response to the management procedures animals decreased their inactive lying and increased their moving activity. Based on behavioural responses the deer did not appear to find the practices increasingly challenging. Behaviour type was the chief influence on interindividual distancing. Differences in distancing between animals were most noticeable while the animals were moving. LAN treatment promoted maintenance of a more normal activity pattern when the animals were disturbed.

Use of long-acting neuroleptics to reduce the stress response to management practices in red deer

Abstract Three groups of six adult red deer hinds were used to determine whether long-acting neuroleptic (LAN) tranquillisers were able to modify the behavioural and physiological responses of the deer to a range of routine management stressors: such drugs may eventually prove useful in capture procedures for wild animals. While the stressors increased moving activity and decreased inactive lying and inter-animal distances, these changes were reduced in LAN-treated animals. Blood samples, collected remotely using automatic equipment, showed that following application of the stressors there was a distinct rise in plasma cortisol concentrations which returned to pre-treatment levels more rapidly in LAN-treated animals. However, there was no effect of LAN treatment on plasma concentrations of creatine phosphokinase (CPK), aspartate aminotransferase (AST), total protein or testosterone. Overall, LAN-treated animals had higher concentrations of plasma thyroxine. Heart rate increased in response to the stressors and while the LAN-treated deer showed a greater increase, as with plasma cortisol concentrations, heart rate returned to baseline more rapidly in these animals. We conclude that LANs may have a role to play in wild deer capture and habituation to farming conditions through a reduction in fear and anxiety, but that further work is required to determine an optimal strategy.

Breed differences in behavioural development in kittens.

Differences in behaviour of pure breed cats have been suggested but not wholly investigated. Oriental/Siamese/Abyssinian (OSA) kittens (n=43) were weekly compared with Norwegian Forest (NFO) kittens (n=39) from the 4th to the 10th week of age in a repeated Open Field Test (OFT) paradigm. Heart rate (HR) and rectal temperature (RT) before and after the test, and behavioural responses during the OFT were recorded. Behaviours registered were analysed by focal animal sampling. Significant breed differences were found; cats of the northern zones (NFO) seem to develop earlier thermoregulatory abilities. Precocious opening of eyes, higher locomotion scores and longer time spent standing, observed in OSA kittens may indicate an earlier neurological development. Inter breed differences recorded for exploration and locomotion seem to indicate coping style divergences: in the OFT challenging situation OSA kittens presented higher emotional tachycardia and performed more passively, with a faster decline in exploration and locomotion scores. NFO kittens exerted a more active behaviour as they spent more time exploring the arena and in escape attempts. Notwithstanding OSA and NFO cat selection was mainly aimed to improve divergent morphological traits, some different behavioural and physiological traits seem to have been maintained or co-selected within each breed.

Stability Assessment of Candidate Reference Genes in Urine Sediment of Prostate Cancer Patients for miRNA Applications

We aimed at assessing the stability of candidate reference genes in urine sediments of men subjected to digital rectal examination for suspected prostate cancer (PCa). Two microRNAs (miR-191 and miR-25) and 1 small nucleolar RNA (SNORD48) were assayed in 35 post-DRE urine sediments of men with PCa and in 26 subjects with histologically confirmed benign prostatic hyperplasia (BPH). The stability of candidate reference genes was assessed through BestKeeper algorithm and equivalence test. miR-200b and miR-452 were used to test for the effect of normalization on target genes. Our results proved miR-191 to be the most stable gene, showing the lowest degree of variation and the highest stability value. miR-25 and SNORD48 values fell beyond the cutoff of acceptability. In conclusion, we recommend the use of miR-191 for normalization purposes in post-DRE urine sediments.

Non-invasive assessment of animal exercise stress: real-time PCR of GLUT4, COX2, SOD1 and HSP70 in avalanche military dog saliva

Exercise has been shown to increase mRNA expression of a growing number of genes. The aim of this study was to assess if mRNA expression of the metabolism- and oxidative stress-related genes GLUT4 (glucose transporter 4), COX2 (cyclooxygenase 2), SOD1 (superoxide dismutase 1) and HSP70 (heat shock protein 70) in saliva changes following acute exercise stress in dogs. For this purpose, 12 avalanche dogs of the Italian Military Force Guardia di Finanza were monitored during simulation of a search for a buried person in an artificial avalanche area. Rectal temperature (RT) and saliva samples were collected the day before the trial (T0), immediately after the descent from a helicopter at the onset of a simulated avalanche search and rescue operation (T1), after the discovery of the buried person (T2) and 2 h later (T3). Expressions of GLUT4, SOD1, COX2 and HSP70 were measured by real-time PCR. The simulated avalanche search and rescue operation was shown to exert a significant effect on RT, as well as on the expression of all metabolism- and oxidative stress-related genes investigated, which peaked at T2. The observed expression patterns indicate an acute exercise stress-induced upregulation, as confirmed by the reductions in expression at T3. Moreover, our findings indicate that saliva is useful for assessing metabolism- and oxidative stress-related genes without the need for restraint, which could affect working dog performance.

RandAgiamo™, a Pilot Project Increasing Adoptability of Shelter Dogs in the Umbria Region (Italy)

Simple Summary In Italy, dog shelters are overcrowded because the rate of dog adoption is lower than that of abandonment. A project called “RandAgiamo” was implemented in a rescue shelter in central Italy. RandAgiamo provides training, socialization and advertising of adult shelter dogs. Official data of the Umbria regional health authorities from the year 2014 showed a higher rate of adoption in shelters involved in the project. RandAgiamo dogs had triple odds of being adopted compared to others housed in shelters of the same province. The increase in adoption rate can be beneficial for both dog welfare and shelter management. Abstract Current Italian legislation does not permit euthanasia of dogs, unless they are ill or dangerous. Despite good intentions and ethical benefits, this “no-kill policy” has caused a progressive overpopulation of dogs in shelters, due to abandonment rates being higher than adoption rates. Shelter overcrowding has negative implications for dog welfare and increases public costs. The aim of this paper is to describe the pilot project “RandAgiamo” implemented in a rescue shelter in the Umbria Region and to evaluate its effectiveness on the rate of dog adoption using official data. RandAgiamo aimed to increase adult shelter dogs’ adoptability by a standard training and socialization programme. It also promoted dogs’ visibility by publicizing them through social media and participation in events. We analysed the official data of the Umbria regional health authorities regarding dog shelters of the Perugia province of the year 2014. In the RandAgiamo shelter, the dog adoption rate was 27.5% higher than that of dogs housed in other shelters located in the same geographical area (p < 0.001). The RandAgiamo project could be beneficial for the dogs’ welfare, owner satisfaction, shelter management, and public perception of shelter dogs. However, staff were required to provide dog training and related activities.

Effects of petting before a brief separation from the owner on dog behavior and physiology: a pilot study

Abstract Human physical contact is known to be effective in decreasing dogs’ level of stress, assessed through endocrine, physiological, and behavioral parameters. Gentle touching has been found to be beneficial for dogs while experiencing or after having experienced a stressful event. The aim of the present study was to assess if dog behavior and physiology during a brief separation from the owner were modified by being petted before owners departure. Ten dogs, not affected by separation‐related problems, were tested twice while separated for 3 minutes from the owner: before separation, dogs once were petted for 1 minute and once were not petted. During each test, dog behavior was measured by continuous sampling and saliva collected 15 minutes after separation for cortisol determination. Findings show that, during both procedures, dogs spent a long time looking for the owner (median 84.5 and 87.5 seconds, respectively) and did not seem highly stressed by separation (low salivary cortisol levels and relatively low intensity and frequency of stress signals). When dogs were petted before separation, they displayed behaviors indicative of calmness for a longer period while waiting for the owners return (Z = −1.955; P = 0.049), and their heart rate showed a marked decrease after the test (Z = −1.682; P = 0.073). This pilot study suggests that petting a dog before a brief separation from the owner may have a positive effect, making the dog calmer during the separation itself. Further studies are needed to analyze more in depth its effectiveness, especially in dogs affected by separation anxiety.

My Dog Is Not My Cat: Owner Perception of the Personalities of Dogs and Cats Living in the Same Household

Simple Summary A growing number of dogs and cats live together, sharing both a common home and common owner. Nevertheless, how do owners of both cats and dogs living in the same household perceive their pets’ personalities? We tried to answer this question by using a questionnaire targeted at people who owned both dogs and cats. Sociability, protectiveness, reactivity, neuroticism, and fearfulness were the traits that emerged and that diversified themselves according to species. Moreover, intrinsic animal factors, such as age and gender, demographic characteristics of the owner, and environmental context seem to modulate the perceived personality traits in a species-specific way. As personality might influence pets’ welfare and adaptability to the home environment, owners of multiple pets should know and take into consideration both common and differential aspects of their pet’s personality to optimise cohabitation among dogs and cats. Abstract This study aims to define the personality traits perceived by the owners of multiple pets and to evaluate how they are modulated by experiential-environmental factors. A questionnaire was administered to 1270 owners of multiple pets (dogs and cats) to collect data on the demographics, management, and personality of their pets. Data were analysed by principal component analysis, bivariate, and multivariable models. Five personality traits emerged in dogs and cats: sociability, reactivity, protectiveness, neuroticism, and fearfulness. The owners perceived differences in the personality of their pet: dogs scored higher in sociability, protectiveness, and reactivity, while lower in the neuroticism dimension compared with cats (p < 0.001). Age similarly affected sociability (p < 0.01), and reactivity (p < 0.001) in both dogs and cats, while species-specific gender differences were found as to fearfulness (p < 0.05) and neuroticism (p < 0.001). The age of acquisition modulated several traits in dog personality, while living with conspecifics especially influenced cats. Physiological, behavioural, and evolutionary characteristics could explain species differences. Moreover, intrinsic and extrinsic factors modulated the five dimensions of dogs and cats in a diversified fashion, suggesting complex interactions between species and the environment. However, owners could have had different attitudes with their animals which could have influenced personality perception.

Next Generation Sequencing of urine exfoliated cells: An approach of prostate cancer microRNAs research

There is emerging evidence that microRNAs (miRNAs) dysregulation is involved in the genesis and the progression of Prostate Cancer (PCa), thus potentially increasing their use in urological clinical practice. This is the first pilot study which utilizes Illumina Deep Sequencing to examine the entire miRNAs spectrum existent in urine exfoliated prostate cells (UEPCs) of PCa patients. A total of 11 male patients with histological diagnosis of PCa were enrolled in the present study. First-catch urine (30 mL) was collected following a prostate massage. Total RNA was extracted from urine and sequenced using an HiSeq2500 System (Illumina). QPCR assay was used to validate the highest NGS results in PCA patients and in age-matched, caucasian men. Remarkably, PCA let-7 family was down-regulated (P < 0.01), compared to the controls. The results of our study support the notion of a relatively high diagnostic value of miRNA family for PCa detection, especially in the let-7 family. The present research confirmed the potential use of miRNAs as non-invasive biomarkers in the diagnosis of PCa, potentially reducing the invasiveness of actual clinical strategy.

Familiarity and Interest in Working with Livestock Decreases the Odds of Having Positive Attitudes towards Non-Human Animals and Their Welfare among Veterinary Students in Italy

Simple Summary Veterinary students are the future generation of veterinary practitioners. Therefore, their attitudes towards non-human animals and animal welfare have a substantial impact on animal welfare itself. This study investigated the attitudes of veterinary students in Italy. The findings suggest that factors such as female gender, familiarity with pets, or the intention to work with pet species, as well as an animal-free diet, and membership in an animal rights association are the predictors of a very positive attitude towards animals and their welfare. Familiarity and intention to work with livestock is instead associated with a less positive attitude. Students also considered the freedom to express normal behaviours and the freedom from fear and distress to be less important for livestock than for pets. Veterinary curricula should take these findings into account to improve the attitudes of students in order to improve animal welfare, especially the welfare of livestock. Abstract We investigated the attitudes of veterinary students towards animals and their welfare in Italy. Regression analyses revealed predictors that are significant in differentiating students’ scoring tendency based on their gender, familiarity, and intention to work with a specific animal species, type of diet, and membership in an animal rights association. Female students, who were mostly familiar with pets and aspired to work with species other than livestock, following an animal-free diet and being a member of an animal rights association, had a significantly greater odds of having a high Animal Attitude Scale score (AAS), i.e., very positive attitude towards animals, versus a less positive attitude. Conversely, the familiarity with livestock and preference for working with livestock significantly increased the odds of a low AAS. Overall, students considered all of the Brambell Report’s Five Freedoms important for animal welfare protection. However, students scored higher for companion animals than for livestock, particularly regarding the freedom to express normal behaviour and the absence of fear and distress. This study suggests that veterinary students place less importance on the psychological aspects of welfare for livestock, and there is a tendency for students who are mostly familiar, or aspire to work, with livestock to have a less positive attitude towards non-human animals and their welfare. These findings should be considered within the veterinary educational curriculum due to their potential impact on animal welfare.