Silvia de Oliveira Hübner

Partial protection induced by a BHV-1 recombinant vaccine against challenge with BHV-5

Abstract: Bovine herpesvirus type 5 (BHV‐5) is the causative agent of bovine herpetic encephalitis, a major concern for cattle farming in Brazil and Argentina. We recently developed a differential, gE‐negative vaccine (265 gE‐), based on a Brazilian BHV‐1 strain. The present study was carried out to examine whether such a vaccine would confer protection to BHV‐5 infections. It was concluded that the recombinant BHV‐1 vaccine tested here is not capable of conferring full protection to BHV‐5 challenge.

Melittin and its potential in the destruction and inhibition of the biofilm formation by Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa isolated from bovine milk

Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa stand out in veterinary and human medicine for their role in opportunistic infections and their pathogenic mechanisms, including the biofilms formation. It was investigated the antibacterial activity of melittin and antibiofilm of such bacteria. Twelve strains of these microorganisms isolated from bovine milk were used, as well as the strains S.xa0aureus ATCC 12600, E.xa0coli ATCC 8739 and Pseudomonas aeruginosa ATCC 15442. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) were determined by broth microdilution technique. The biofilms were formed in 96-well plates and melittin on these colonies was added at different concentrations and times. Bacteria previously exposed to melittin were evaluated for inhibition of biofilm production. The MIC and MBC were respectively in μg/mL: S.xa0aureus (6-7 and 32-64), E.xa0coli (40-42.5 and 64-128) and P.xa0aeruginosa (65-70 and 64-128). S.xa0aureus biofilms were more sensitive to the action of melittin, since upon exposure to a concentration 10 times lower than the MIC for 4xa0h, was completely destroyed. In Gram negative bacteria, the pre-formed biofilm was destroyed only when exposed for 4xa0h under the MIC. With respect to inhibition of biofilm production, S.xa0aureus was the most sensitive again because produced only 37.2% of the biofilm formed by the control (without previous exposure to melittin), when exposed to the MIC, and at a concentration hundred times smaller than MIC, this microorganism produced 75.2% of the biofilm. E.xa0coli was the most resistant bacteria and produced 56.3% of the biofilm, even if previously exposed to melittin MIC. Melittin presents desirable effects in combating microorganisms studied both at your disposal, biofilm destruction and inhibition of the formation, and maybe used in future studies of new strategies to combat infections caused by these pathogens.