Silvina Mariela Vidueiros

Nutrition disorder and immunologic parameters: study of the intestinal villi in growing rats

OBJECTIVE The aim of the present work was to study how a diet in which cereals were the only protein source would affect B and T lymphocytes and a cell population positive for thymus-expressed chemokine (TECK) in the intestinal villi of growing rats. METHODS Wistar rats were fed a 6.5% precooked maize protein diet for 18-20 d (M group). An age-matched control group received stock diet (C group). Body weight (grams) was determined, ponderal growth rate (grams per day per 100 g) was calculated, and intestines were removed and processed by Saint-Maries technique. Tissue sections were studied by indirect immunofluorescence. CD5(+) T cells and the T-cell subsets TCRalphabeta(+), TCRgammadelta(+), CD4(+), CD8alpha(+), and CD8beta(+) in the lamina propria (LP) and intraepithelium, in addition to immunoglobulin A-positive B cells in the LP were determined (n cells/30 fields were read). In addition, the presence of the TECK(+) cell population was qualitatively assessed. RESULTS The M versus C group showed statistically significant differences in body weight and ponderal growth rate. The number of immunoglobulin A-positive B cells in the LP and the CD5(+) T cells and CD4(+), CD8alpha(+), CD8beta(+), TCRalphabeta(+), and TCRgammadelta(+) T-cell subpopulations of the M group in the LP and intraepithelium of the gut villi were significantly decreased compared with the C group (P < 0.001). The M group also showed differences in the size and cellularity of the gut villi and in the distribution of TECK. CONCLUSION The results show that intake of a low concentration of a low-quality dietary protein as the only source of protein produces an important disorder in the mucosal immunity of experimental rats.

DETERMINACIÓN DEL CONTENIDO DE GLIADINAS EN ALIMENTOS ELABORADOS CON AMARANTO, QUÍNOA Y/O CHÍA

Resumen es: Amaranto, quinoa y chia, por ser naturalmente libres de gluten, pueden ser incorporados en la dieta celiaca. Con el objeto de evaluar una posible contami...

Effect of a quinoa (Chenopodium quinoa W) based diet on the intestinal mucosa of growing Wistar rats

.The aim of the present study was to Þnd out the effect of a variety of quinoa from Campo Tapial de Colanzuli, Iruya, Salta, withoutwashing treatment, on the intestinal mucosa of growing rats.In this study, Wistar rats ( n = 6/group) from weaning to 7 days fed a 10% protein diet with 1) unwashed quinoa (Q), 2) commercialwashed quinoa (QR), and 3) casein (C) as control group. Body weight (BW, g) and diet intake (I, g/day) were determined, and PonderalGrowth Rate (PGR, g/day/100g) was calculated. Intestines were removed, processed by Saint Marie «s technique and stained with AlcianBlue-H/E. Goblets cells/100 epithelial cells (GC) were determined in 10 intestinal villi/rat. Tissue sections were studied by indirectimmunosuorescence technique