Simon J. MacKenzie
University of Glasgow
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Featured researches published by Simon J. MacKenzie.
The EMBO Journal | 1999
Ralf Hoffmann; George S. Baillie; Simon J. MacKenzie; Stephen J. Yarwood; Miles D. Houslay
The extracellular receptor stimulated kinase ERK2 (p42MAPK)‐phosphorylated human cAMP‐specific phosphodiesterase PDE4D3 at Ser579 and profoundly reduced (∼75%) its activity. These effects could be reversed by the action of protein phosphatase PP1. The inhibitory state of PDE4D3, engendered by ERK2 phosphorylation, was mimicked by the Ser579→Asp mutant form of PDE4D3. In COS1 cells transfected to express PDE4D3, challenge with epidermal growth factor (EGF) caused the phosphorylation and inhibition of PDE4D3. This effect was blocked by the MEK inhibitor PD98059 and was not apparent using the Ser579→Ala mutant form of PDE4D3. Challenge of HEK293 and F442A cells with EGF led to the PD98059‐ablatable inhibition of endogenous PDE4D3 and PDE4D5 activities. EGF challenge of COS1 cells transfected to express PDE4D3 increased cAMP levels through a process ablated by PD98059. The activity of the Ser579→Asp mutant form of PDE4D3 was increased by PKA phosphorylation. The transient form of the EGF‐induced inhibition of PDE4D3 is thus suggested to be due to feedback regulation by PKA causing the ablation of the ERK2‐induced inhibition of PDE4D3. We identify a novel means of cross‐talk between the cAMP and ERK signalling pathways whereby cell stimuli that lead to ERK2 activation may modulate cAMP signalling.
British Journal of Pharmacology | 2002
Simon J. MacKenzie; George S. Baillie; Ian McPhee; Carolynn MacKenzie; Rachael Seamons; Theresa McSorley; Jenni Millen; M. Beard; Gino Van Heeke; Miles D. Houslay
Challenge of COS1 cells with the adenylyl cyclase activator forskolin led to the activation of recombinant PDE4A8, PDE4B1, PDE4C2 and PDE4D5 cAMP‐specific phosphodiesterase long isoforms. Forskolin challenge did not activate mutant long PDE4 isoforms where the serine target residue (STR) within the protein kinase A (PKA) consensus phosphorylation site in Upstream Conserved Region 1 (UCR1) was mutated to alanine. The PKA inhibitor, H89, ablated forskolin activation of wild‐type long PDE4 isoforms. Activated PKA caused the in vitro phosphorylation of recombinant wild‐type long PDE4 isoforms, but not those where the STR was mutated to alanine. An antiserum specific for the phosphorylated form of the STR detected a single immunoreactive band for recombinant long PDE4 isoforms expressed in COS1 cells challenged with forskolin. This was not evident in forskolin‐challenged cells treated with H89. Neither was it evident in forskolin‐challenged cells expressing long isoforms where the STR had been mutated to alanine. In transfected COS cells challenged with forskolin, only the phosphorylated PDE4D3 long form showed a decrease in mobility in Western blotting analysis. This decreased mobility of PDE4D3 was ablated upon mutation of either of the two serine targets for PKA phosphorylation in this isoform, namely Ser54 in UCR1 and Ser13 in the isoform‐specific N‐terminal region. Activation by forskolin challenge did not markedly alter the sensitivity of PDE4A8, PDE4B1, PDE4C2 and PDE4D5 to inhibition by rolipram. Long PDE4 isoforms from all four sub‐families can be phosphorylated by protein kinase A (PKA). This leads to an increase in their activity and may thus contribute to cellular desensitization processes in cells where these isoforms are selectively expressed.
British Journal of Pharmacology | 2000
George S. Baillie; Simon J. MacKenzie; Ian McPhee; Miles D. Houslay
Expressed in intact cells and in vitro, PDE4B and PDE4C isoenzymes of cyclic nucleotide phosphodiesterase (PDE), in common with PDE4D isoenzymes, are shown to provide substrates for C‐terminal catalytic unit phosphorylation by the extracellular signal‐regulated kinase Erk2 (p42MAPK). In contrast, PDE4A isoenzymes do not provide substrates for C‐terminal catalytic unit phosphorylation by Erk2. Mutant PDE4 enzymes were generated to show that Erk2 phosphorylation occurs at a single, cognate serine residue located within the C‐terminal portion of the PDE4 catalytic unit. PDE4 long‐form isoenzymes were markedly inhibited by Erk2 phosphorylation. The short‐form PDE4B2 isoenzyme was activated by Erk2 phosphorylation. These functional changes in PDE activity were mimicked by mutation of the target serine for Erk2 phosphorylation to the negatively charged amino acid, aspartic acid. Epidermal growth factor (EGF) challenge caused diametrically opposed changes in cyclic AMP levels in COS1 cells transfected to express the long PDE4B1 isoenzyme compared to cells expressing the short PDE4B2 isoenzyme. We suggest that PDE4 enzymes may provide a pivotal point for integrating cyclic AMP and Erk signal transduction in cells with 4 genes encoding enzymes that are either insensitive to Erk2 action or may either be activated or inhibited. This indicates that PDE4 isoenzymes have distinct functional roles, giving credence to the notion that distinct therapeutic benefits may accrue using either PDE4 subfamily or isoenzyme‐selective inhibitors.
Biochemical Journal | 2000
Simon J. MacKenzie; Miles D. Houslay
U937 monocytic cells are shown here to express a range of PDE4, cAMP-specific phosphodiesterase (PDE) isoenzymes: the long isoenzymes, PDE4A4, PDE4D5 and PDE4D3, plus the short isoenzyme, PDE4B2. These isoenzymes provide around 76% of the total cAMP PDE activity of U937 cells. The specific activities of the total PDE4A, PDE4B and PDE4D activities were 0.63+/-0.09, 8.8+/-0.2 and 34.4+/-2.9 pmol/min per mg of protein respectively. The PDE4 selective inhibitor, rolipram, inhibited immunopurified PDE4B and PDE4D activities similarly, with IC(50) values of approx. 130 nM and 240 nM respectively. In contrast, rolipram inhibited immunopurified PDE4A activity with a dramatically lower IC(50) value of around 3 nM. Rolipram increased phosphorylation of cAMP-response-element-binding protein (CREB) in U937 cells in a dose-dependent fashion, which implied the presence of both high affinity (IC(50) value approx. 1 nM) and low affinity (IC(50) value approx. 120 nM) components. Rolipram dose-dependently inhibited the interferon-gamma (IFN-gamma)-stimulated phosphorylation of p38 mitogen-activated protein (MAP) kinase in a simple monotonic fashion with an IC(50) value of approx. 290 nM. On this basis, it is suggested that rolipram inhibition of PDE4A4 is involved in regulating CREB phosphorylation but not IFN-gamma-stimulated p38 MAP kinase phosphorylation. PDE4A4 was also selectively activated by challenge of U937 cells with either bacterial lipopolysaccharide (LPS) or IFN-gamma through a process which was attenuated by both wortmannin and rapamycin. It is proposed that the PDE4A4 isoform is involved in compartmentalized cAMP signalling responses in U937 monocytes.
Bioorganic & Medicinal Chemistry Letters | 2008
Mark W. Irvine; Graham L. Patrick; Justin Kewney; Stuart F. Hastings; Simon J. MacKenzie
The discovery, synthesis and in vitro activity of a novel series of rhodanine based phosphodiesterase-4 (PDE4) inhibitors is described. Structure-activity relationship studies directed toward improving potency led to the development of submicromolar inhibitors 2n and 3i (IC(50)=0.89 & 0.74 microM). The replacement of rhodanine with structurally related heterocycles was also investigated and led to the synthesis of pseudothiohydantoin 7 (IC(50)=0.31 microM).
European Journal of Pharmacology | 2006
Lucien Charles Donald Gibson; Stuart F. Hastings; Ian McPhee; Robert A. Clayton; Claire E. Darroch; Alison Mackenzie; Fiona L. MacKenzie; Michiaki Nagasawa; Patricia A. Stevens; Simon J. MacKenzie
Molecular Pharmacology | 2001
George S. Baillie; Simon J. MacKenzie; Houslay
Biochemical Society Transactions | 2005
Ian McPhee; L.C.D. Gibson; J. Kewney; C. Darroch; P.A. Stevens; D. Spinks; A. Cooreman; Simon J. MacKenzie
Proceedings of the National Academy of Sciences of the United States of America | 1998
Simon J. MacKenzie; Stephen J. Yarwood; Alexander H. Peden; Graeme B. Bolger; Richard G. Vernon; Miles D. Houslay
Biochemical Journal | 1998
Iona Fleming; Simon J. MacKenzie; Richard G. Vernon; Neil G. Anderson; Miles D. Houslay; Elaine Kilgour