Simon Officer

Variables to be considered when assessing the photocatalytic destruction of bacterial pathogens

The current study sought to assess the importance of three common variables on the outcome of TiO(2) photocatalysis experiments with bacteria. Factors considered were (a) ability of test species to withstand osmotic pressure, (b) incubation period of agar plates used for colony counts following photocatalysis and (c) chemical nature of suspension medium used for bacteria and TiO(2). Staphylococcus aureus, Escherichia coli, Salmonella ser. Typhimurium and Pseudomonas aeruginosa were found to vary greatly in their ability to withstand osmotic pressure, raising the possibility that osmotic lysis may be contributing to loss of viability in some photocatalytic disinfection studies. Agar plate incubation time was also found to influence results, as bacteria treated with UV light only grew more slowly than those treated with a combination of UV and TiO(2.) The chemical nature of the suspension medium used was found to have a particularly pronounced effect upon results. Greatest antibacterial activity was detected when aqueous sodium chloride solution was utilised, with approximately 1 x 10(6) CFU mL(-1)S. aureus being completely killed after 60 min. Moderate activity was observed when distilled water was employed with bacteria being killed after 2h and 30 min, and no antibacterial activity at all was detected when aqueous tryptone solution was used. Interestingly, the antibacterial activity of UV light on its own appeared to be very much reduced in experiments where aqueous sodium chloride was employed instead of distilled water.

Novel online security system based on rare-earth-doped glass microbeads

A novel fluorescent security label has been produced that could replace numerous conventional fluorescent dyes in document security. This label utilizes rare earth ions doped in a borosilicate glass matrix to produce sharp spectral fluorescence peaks with characteristic long lifetimes due to the rare earth ions. These are subsequently detected by an online detection system based on fluorescence and the long lifetimes to avoid any interference from other fluorophores present in the background. Security is further enhanced by the interaction of the rare earth ions with each other and the effect of the host on the emission spectra and therefore the number of permutations that could be produced. This creates a very secure label with various applications for the security market.

Improvement in fingerprint detection using Tb(III)-dipicolinic acid complex doped nanobeads and time resolved imaging

This paper deals with the synthesis and application of lanthanide complex doped nanobeads used as a luminescent fingerprint powder. Due to their special optical properties, namely a long emission lifetime, sharp emission profiles and large Stokes shifts, luminescent lanthanide complexes are useful for discriminating against signals from background emissions. This is a big advantage because latent fingerprints placed on multicoloured fluorescent surfaces are difficult to develop with conventional powders. The complex of 2,6-dipicolinic acid (DPA) and terbium ([Tb(DPA)3](3-)) is used for this purpose. Using the Stöber process, this complex is incorporated into a silica matrix forming nanosized beads (230-630nm). It is shown that the [Tb(DPA)3](3-) is successfully incorporated into the beads and that these beads exhibit the wanted optical properties of the complex. A phenyl functionalisation is applied to increase the lipophilicity of the beads and finally the beads are used to develop latent fingerprints. A device for time resolved imaging was built to improve the contrast between developed fingerprint and different background signals, whilst still detecting the long lasting luminescence of the complex. The developed fingerprint powder is therefore promising to develop fingerprints on multicoloured fluorescent surfaces.

Rare Elements Electrochemistry: The Development of a Novel Electrochemical Sensor for the Rapid Detection of Europium in Environmental Samples Using Gold Electrode Modified with 2-pyridinol-1-oxide.

This work presents for the first time the electrochemical determination of europium using cyclic voltammetry at gold electrodes modified with 2-pyridinol-1-oxide. A well-defined oxidation peak was observed in cyclic voltammetry as a result of the oxidation of the europium at ∼1100 mV in phosphate buffer at pH 7.0. The peak current increased linearly with the increase of concentration of the europium over the range from 1 to 80 μM and detection limit (based on 3-sigma) and quantification were found to be 0.3 and 0.549 μM, respectively. The analytical utility of the developed protocol was evaluated by performing the detection of the europium in river water. Europium is also linear over the concentration range 10 to 150 μM. (I(p)/μA = 0.7239x + 108.19, R(2) = 0.9981 and n = 9) with a detection limit of 6.5 μM (based on 3-sigma). This simple and effective protocol exhibited good sensitivity, precision and reliability towards the detected analyte.

Sensitive Novel Fluorescent Tracers for Environmental Monitoring

This paper discusses the development and use of novel glass and polymer tracers, based on narrow band atomic fluorescence, which have been developed for deployment as environmental tracers. The use of discrete fluorescent species in an environmentally stable host has been developed to replace existing toxic, broad band molecular dye tracers. The narrow band emission signals offer the potential for the tracing of a large numbers of signals in the same environment. This will give significant competitive advantage and increased data accuracy and also allow multiple source environmental monitoring of environmental parameters. These novel environmental tracers exhibiting highly discrete fluorescent emissions can also be formed into many shapes, forms and densities to mimic naturally occurring media.

Waveguide-based machine readable fluorescence security feature for border control and security applications

Border security challenges and immigration issues are increasing considerably in recent years. Counterfeiting and fraudulent use of identity and other travel documents are posing serious threats and safety concerns worldwide, ever since the advancement of computers, photocopiers, printers and scanners. Considering the current scenario of illegal migration and terrorism across the world, advanced technologies and improved security features are essential to enhance border security and to enable smooth transits. In this paper, we present a novel dual waveguide based invisible fluorescence security feature and a simple validation system to elevate and strengthen the security at border controls. The validation system consists of an LED (light emitting diode) as excitation source and an array photodetector which helps in the simultaneous detection of multiple features from the fluorescence waveguides. The fluorescence waveguides can be embedded into the identity document as micro-threads or tags which are invisible to the naked eye and are only machine readable. In order to improve the sensitivity, rare earth fluorescence materials are used which absorb only specific ultraviolet (UV) or visible (VIS) wavelengths to create corresponding fluorescent emission lines in the visible or infrared wavelengths. Herein, we present the preliminary results based on the fluorescence spectroscopic studies carried out on the fabricated rare earth doped waveguides. The effect of different rare earth concentrations and excitation wavelengths on the fluorescence intensity were investigated.