Simona Normando

Effect of manual imitation of grooming on riding horses' heart rate in different environmental situations.

S. Normando1*, A. Haverbeke2,4, L. Meers2, F.O. Ödberg2, M. Ibáñez Talegón3 and G. Bono1 1Dipartimento di Scienze Sperimentali Veterinarie, Università di Padova, V iale dell’Università 16, Agripolis, I-35020 L egnaro PD, Italy; 2Department of Animal Nutrition, Genetics, Production and Ethology, Universiteit Gent, Heidestraat 19, B-9820 Merelbeke, Belgium; 3Departamento de Producción Animal, Universidad Complutense de Madrid, Ciudad Universitaria, E-28040 Madrid, España; 4Present address: FUNDP, Department of Veterinary Medicine, rue Muzet, 4 B-5000 Namur, Belgium *Correspondence: Dipartimento di Scienze Sperimentali Veterinarie, Università di Padova, Strada Romea 16, Agripolis, I-35020 L egnaro PD, Italy E-mail: simona.normando@unipd.it

Can novel methods be useful for pain assessment of castrated piglets

Given that surgical castration is a painful practice performed on millions of pigs every year, a need to identify novel reliable pain assessment tools exists in order to test anaesthetic and analgesic protocols that may reduce related pain. Two treatments were considered: handling (H) and surgical castration (C). Physiological (cortisol, lactate, glycaemia, rectal and eye temperature) and behavioural variables (latency to move after treatment and alterations in posture and walking) were analysed. Cortisol showed the greatest level in C piglets within 20 min after the surgical procedure and a positive correlation with glucose concentration. Eye temperature was higher in C piglets, and the same difference was detected for rectal temperature 3 h after castration. Behavioural parameters revealed that C piglets had longer latency to move and a higher percentage of them showed alterations in posture and walking. Results of this study showed that, in castrated piglets behavioural and physiological alterations occur mainly in the first 3 h from treatment. Latency to move, alterations in posture and walking, and eye temperature appear to give additional and useful information in piglet pain assessment. However, differently from the behavioural parameters considered, eye temperature involves several manipulations of the animals and a long process to acquire the data.

Dog assisted interventions in a specialized centre and potential concerns for animal welfare

Dog assisted interventions in a specialized centre and potential concerns for animal welfare L. Marinelli & S. Normando & C. Siliprandi & M. Salvadoretti & P. Mongillo Published online: 4 July 2009 # Springer Science + Business Media B.V. 2009

Does Subjective Rating Reflect Behavioural Coding? Personality in 2 Month-Old Dog Puppies: An Open-Field Test and Adjective-Based Questionnaire

A number of studies have recently investigated personality traits in non-human species, with the dog gaining popularity as a subject species for research in this area. Recent research has shown the consistency of personality traits across both context and time for adult dogs, both when using questionnaire based methods of investigation and behavioural analyses of the dogs’ behaviour. However, only a few studies have assessed the correspondence between these two methods, with results varying considerably across studies. Furthermore, most studies have focused on adult dogs, despite the fact that an understanding of personality traits in young puppies may be important for research focusing on the genetic basis of personality traits. In the current study, we sought to evaluate the correspondence between a questionnaire based method and the in depth analyses of the behaviour of 2-month old puppies in an open-field test in which a number of both social and non-social stimuli were presented to the subjects. We further evaluated consistency of traits over time by re-testing a subset of puppies. The correspondence between methods was high and test- retest consistency (for the main trait) was also good using both evaluation methods. Results showed clear factors referring to the two main personality traits ‘extroversion,’ (i.e. the enthusiastic, exuberant approach to the stimuli) and ‘neuroticism,’ (i.e. the more cautious and fearful approach to the stimuli), potentially similar to the shyness-boldness dimension found in previous studies. Furthermore, both methods identified an ‘amicability’ dimension, expressing the positive interactions the pups directed at the humans stranger, and a ‘reservedness’ dimension which identified pups who largely chose not to interact with the stimuli, and were defined as quiet and not nosey in the questionnaire.

DISTANCES BETWEEN INDIVIDUALS IN AN ARTIFICIAL HERD OF AFRICAN ELEPHANTS (LOXODONTA AFRICANA AFRICANA) DURING RESOURCE UTILISATION IN A SEMI-CAPTIVE ENVIRONMENT

Space allowance and resource dispersion is recognised as an important factor affecting the welfare of elephants in captivity. In the present pilot study, we investigated distances kept among individuals in an artificially created semi-captive mixed-sex group of African elephants, when individuals were free to disperse. The study involved a herd of six elephants, three females (aged 11 to 16years), and three males (aged 15 to 23years). They were observed through instantaneous scan sampling in order to assess distances between individuals and body orientation in space and through continuous focal animal sampling to assess inter-specific social behaviour and general activity. A total of 312 suitable scans were collected for evaluation of distances between individuals. While foraging in absence of discernible space constraints, elephants maintained a distance equalling five or more body lengths in 63.9% of the scans, with wide differences between dyads. Little social behaviour, mainly affiliative, was recorded. The results of this pilot study suggest further scientific investigation could help to understand whether placing resources at five body lengths distance or over in a controlled environment could increase their simultaneous utilisation by all members of a group and contribute to decrease aggression. However, caution is warranted when applying results to different groups, environments and management regimes.

Evaluation of behaviour in stabled draught horse foals fed diets with two protein levels

The present work is aimed at evaluating the behaviour of Italian Heavy Draught Horse (IHDH) foals reared in semi-covered stables and fed two isoenergetic total mixed rations with different dietary protein levels (13.2% and 10.6% of CP on dry matter). The study was prompted by the restrictions for nitrate emissions in farms of the European Nitrate Directive. One suggested solution is to reduce dietary protein while maintaining normal performance and welfare, but there is a lack of literature in studies of horses. The behaviours of 20 foals of 437±60 kg of BW, aged 379±37 days and stabled in four pens by sex (S) and diet (D) were video recorded and analysed to build a suitable ethogram including 18 behaviours in six categories: ingestion, resting, maintenance, movement, social activities, other. The percentage of the daily time spent in each behavioural category and single behaviours was analysed via a single traits GLM including S, D and their interaction. Daily activity was consistent with existing literature: foals spent about 33% of the day in ingestion activities and 41% in resting, whereas social interactions constituted 8% of the time and individual maintenance <2%. Concerning diet, foals fed high protein spent more time in movement (19.62±0.73% of day v. 10.45±0.73% in low-protein (LP) foals; P⩽0.001), whereas the LP group increased resting (43.42±1.12% v. 38.02±1.12%; P⩽0.001). No stereotypies were found, and daily activity followed the typical values for draught breeds for foals in both dietary groups, a result that suggests the maintenance of well-being after dietary protein reduction. This result, together with the findings of a companion study showing no changes in growth performances of foals, showed that a reduction of CP in foal diet is reconcilable with the maintenance of performance and welfare.

IMPROVING UPPER ELEMENTARY STUDENTS' HUMANE ATTITUDES AND PROSOCIAL BEHAVIORS THROUGH AN IN-CLASS HUMANE EDUCATION PROGRAM

ABSTRACT Humane education is a long-standing field of education that endeavors to nurture kindness, compassion, and concern for nonhuman animals, people, and the environment. Despite its long history, however, few randomized control studies have evaluated its effectiveness to promote the development of relevant outcomes. The current study sought to address this dearth by investigating the effects of a humane education program on not only participating students’ attitudes, but also their behaviors. Classes of under-served, fourth-grade students in two major US cities were randomly assigned to participate in either a school-based humane education program or a chess club (the control group); all students participated in their respective activity during the same period once a week for 11 weeks. Data were collected on the attitudes (n = 236) and behaviors (n = 167) of participating students exactly one week before and exactly one week after these programs were conducted. Students self-reported their attitudes about the treatment of animals and the environment via the Intermediate Attitude Scale. Teachers rated each students’ prosocial and disruptive behaviors through sub-scales of the Teacher Observation of Classroom Adaptation– Checklist. Nested multilevel models of change found that the development of prosocial behaviors and self-reported attitudes significantly interacted with group assignment: Students who participated in the humane education program showed stronger growth in both of these outcomes compared with students in the control group. Changes in disruptive behaviors, how-ever, were not found to differ between groups. The results support the effectiveness of a humane education program to teach a relatively large and diverse group of upper elementary students to learn about animal welfare issues and to improve their prosocial behaviors. Effects appeared strongest on attitudes; behavioral effects were found to be largely limited to behaviors directly addressed by the humane education program.

Effects of a delay in feed delivery on behaviour, milk yield and heamatological parameters of dairy cows

This study aimed to evaluate the effects of a delay (at 09:30 instead of 07:30 h) in Total Mixed Ration (TMR) delivery on the behaviour, milk yield, and haematological parameters in dairy cows. Twelve healthy Italian Holstein cows, divided into two homogeneous groups of six each, were involved. The TMR delay was applied either for one (on Tuesday; single delay, SD) or for three consecutive days (Tuesday to Thursday; repeated delay, RD) to one group at a time while the other acted as control. A week of pause was interposed between treatments and the order of the treatment was randomised. Behaviour was observed by instantaneous scan sampling every 2 min during seven half-hour periods on Tuesday and Thursday and blood samples were taken at 14:30 h on these days. Milk yield was recorded daily. Data were analysed by a mixed models for repeated measurements. The delay caused a marked increase in activities towards the feeding rack prior to feeding on day 1 when delay was applied both as SD (P<0.01) and RD (P<0.05). On the third day of delay, pre- and post-meal agonistic behaviour increased in delayed cows (P<0.01). Milk yield and blood parameters were not affected by the delay. Results suggest that a two hours delay in feeding can affect behaviour in cows, increasing behavioural patterns, which may be a sign of mild frustration.

Factors Influencing the Quality of Life of the Cat in its Relationship with Owners

In a multiple study between veterinarians and psychologists the owner–pet relationship was evaluated, focusing attention on the problems and the benefits due to the relationship (Bono, 2000; Camperio Ciani 2000; Bono et al., 2003) and a multiple approach method was validated to assess the quality of life of the pet in its relationship with the owner (Marinelli et al., 2001). The aim of this study was to analyse the relationship between cats and their owners and identify factors that influence the quality of life of the cat.

Assessing Animal Welfare in Animal-Visitor Interactions in Zoos and Other Facilities. A Pilot Study Involving Giraffes

Simple Summary A pilot test of a six-step protocol to evaluate animal-visitor interactions was developed on a “giraffe feeding” program in a zoo. The steps devoted to animal welfare’s assessment are presented in this paper. We observed the giraffes’ behaviour, evaluated the suitability of the area in which the interactions took place, and assessed the intensity of various hazards for animal welfare. The long-term goal of this research project is to test a protocol for the overall evaluation of the quality of animal-visitor interactions in zoos and other facilities. Giraffes could choose whether to participate or not to the feeding interactions with visitors, and did not show any behaviour that suggested they did find the interaction to be a negative experience, so their welfare level was assessed as not to be adversely affected by the interaction with the visitors. Abstract In recent years, awareness of the controversial aspects connected with wild animal-visitor interactions (AVIs) in zoos and other facilities has increased due to cultural changes. Therefore, the need to apply transparent procedures to evaluate AVIs programs in zoos and similar facilities has also increased. This study presents results of animal welfare’s assessment of a pilot test of a protocol based on six steps that aim to explore and assess the overall value of AVIs considering the impact both on animals and visitors. In the present paper, we discuss the multifaceted approach to animal welfare assessment during animal-visitor interactions, combining quantitative behavioural observations/analysis and a welfare risk-assessment procedure, which forms the basis of the six-step protocol. Pilot testing of said approach to animal welfare assessment involved giraffes (Giraffa camelopardalis) in an Italian zoo. No change in behaviour, suggestive of an increased welfare risk to the animals, was found. The risk analysis reported overall low risks for welfare, whereas enclosure analysis highlighted that the enclosure was suitable for allowing interactions without jeopardising animal welfare, mainly because it allowed animals to choose whether to interact or withdraw from interactions without decreasing the space available to them.