Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Simona Scardala is active.

Publication


Featured researches published by Simona Scardala.


Food and Chemical Toxicology | 2012

Contamination by Microcystis and microcystins of blue-green algae food supplements (BGAS) on the Italian market and possible risk for the exposed population.

Susanna Vichi; Paolo Lavorini; Enzo Funari; Simona Scardala; Emanuela Testai

Blue green algae supplements (BGAS) are generally proposed as health-promoting natural products for their purported beneficial effects. Spirulina spp. and Aphanizomenon flos aquae are mainly used in BGAS production. They are usually collected from the natural environment, where other potentially toxic cyanobacteria can be present, making possible BGAS contamination by cyanotoxins, with potential risk for human health. In this work we apply a combined approach, by using chemical and molecular techniques, on BGAS of 17 brands available in Italy. Samples containing Spirulina-only were free of contamination. The Aphanizomenon flos aquae-based samples were contaminated by highly variable levels of microcystins (MC-LR and MC-LA congeners), up to 5.2 μg MC-LR equivalents per gram product. The highest variability (up to 50 fold) was among batches of the same brand, although intra-batch differences were also evidenced. PCR analyses were positive only for the presence of Microcystis sp., identified as the toxin-producing species responsible for contamination. At the maximum contamination levels found, a risk for consumers can be expected following chronic or sub-chronic exposure to a reasonable daily BGAS consumption of 4 g. The need for a strict monitoring by producers and Health Authority to assure an adequate protection for consumers is underscored.


Archives of Toxicology | 2017

Cyanotoxins: producing organisms, occurrence, toxicity, mechanism of action and human health toxicological risk evaluation

Franca M. Buratti; Maura Manganelli; Susanna Vichi; Mara Stefanelli; Simona Scardala; Emanuela Testai; Enzo Funari

Abstract Cyanobacteria were present on the earth 3.5 billion years ago; since then they have colonized almost all terrestrial and aquatic ecosystems. They produce a high number of bioactive molecules, among which some are cyanotoxins. Cyanobacterial growth at high densities, forming blooms, is increasing in extension and frequency, following anthropogenic activities and climate changes, giving rise to some concern for human health and animal life exposed to cyanotoxins. Numerous cases of lethal poisonings have been associated with cyanotoxins ingestion in wild animal and livestock. In humans few episodes of lethal or severe human poisonings have been recorded after acute or short-term exposure, but the repeated/chronic exposure to low cyanotoxin levels remains a critical issue. The properties of the most frequently detected cyanotoxins (namely, microcystins, nodularins, cylindrospermopsin and neurotoxins) are here critically reviewed, describing for each toxin the available information on producing organisms, biosynthesis/genetic and occurrence, with a focus on the toxicological profile (including kinetics, acute systemic toxicity, mechanism and mode of action, local effects, repeated toxicity, genotoxicity, carcinogenicity, reproductive toxicity; human health effects and epidemiological studies; animal poisoning) with the derivation of health-based values and considerations on the risks for human health.


Chemical Research in Toxicology | 2011

Human glutathione transferases catalyzing the conjugation of the hepatoxin microcystin-LR.

Franca M. Buratti; Simona Scardala; Enzo Funari; Emanuela Testai

Many cyanobacterial species are able to produce cyanotoxins as secondary metabolites. Among them, microcystins (MC) are a group of around 80 congeners of toxic cyclic heptapeptides. MC-LR is the most studied MC congener, in view of its high acute hepatotoxicity and tumor promoting activity. Humans may be exposed to cyanotoxins through several routes, the oral one being the most important. The accepted pathway for MC-LR detoxication and excretion in the urine is GSH conjugation. The GSH adduct (GS-MCLR) formation has been shown to occur spontaneously and enzymatically, catalyzed by glutathione transferases (GSTs). The enzymatic reaction has been reported but not characterized both in vitro and in vivo in animal and plant species. No data are available on humans. In the present work, the MC-LR conjugation with GSH catalyzed by five recombinant human GSTs (A1-1, A3-3, M1-1, P1-1, and T1-1) has been characterized for the first time. All GSTs are able to catalyze the reaction; kinetic parameters K(m), k(cat), and their relative specific activities to form GS-MCLR were derived (T1-1 > A1-1 > M1-1 > A3-3 ≫ P1-1). In the range of MC tested concentrations used (0.25-50 μM) GSTT1-1 and A1-1 showed a typical saturation curve with similar affinity for MC-LR (≈80 μM; k(cat) values 0.18 and 0.10 min(-1), respectively), A3-3 and M1-1 were linear, whereas GSTP1-1 showed a temperature-dependent sigmoidal allosteric curve with a k(cat) = 0.11 min(-1). The enzymes mainly expressed in the liver and gastrointestinal tract, GSTA1-1, T1-1, and M1-1, seemed to be mainly involved in the MC-LR detoxification after oral exposure, whereas P1-1 kinetics and location in the skin suggest a role related to dermal exposure. Considering the high frequency of some GST polymorphism, especially M1 and T1 gene deletion, with complete loss in activity, this information could be the first step to identify groups of individual at higher risk associated with MC exposure.


Water Research | 2010

Health risk evaluation associated to Planktothrix rubescens: An integrated approach to design tailored monitoring programs for human exposure to cyanotoxins

Maura Manganelli; Simona Scardala; Mara Stefanelli; Susanna Vichi; Daniela Mattei; Sara Bogialli; Piegiorgio Ceccarelli; Ernesto Corradetti; Ines Petrucci; Simonetta Gemma; Emanuela Testai; Enzo Funari

Increasing concern for human health related to cyanotoxin exposure imposes the identification of pattern and level of exposure; however, current monitoring programs, based on cyanobacteria cell counts, could be inadequate. An integrated approach has been applied to a small lake in Italy, affected by Planktothrix rubescens blooms, to provide a scientific basis for appropriate monitoring program design. The cyanobacterium dynamic, the lake physicochemical and trophic status, expressed as nutrients concentration and recycling rates due to bacterial activity, the identification/quantification of toxic genotype and cyanotoxin concentration have been studied. Our results indicate that low levels of nutrients are not a marker for low risk of P. rubescens proliferation and confirm that cyanobacterial density solely is not a reliable parameter to assess human exposure. The ratio between toxic/non-toxic cells, and toxin concentrations, which can be better explained by toxic population dynamic, are much more diagnostic, although varying with time and environmental conditions. The toxic fraction within P. rubescens population is generally high (30-100%) and increases with water depth. The ratio toxic/non-toxic cells is lowest during the bloom, suggesting a competitive advantage for non-toxic cells. Therefore, when P. rubescens is the dominant species, it is important to analyze samples below the thermocline, and quantitatively estimate toxic genotype abundance. In addition, the identification of cyanotoxin content and congeners profile, with different toxic potential, are crucial for risk assessment.


Annali dell'Istituto Superiore di Sanità | 2012

Emerging health issues of cyanobacterial blooms

Maura Manganelli; Simona Scardala; Mara Stefanelli; Francesca Palazzo; Enzo Funari; Susanna Vichi; Franca M. Buratti; Emanuela Testai

This paper describes emerging issue related to cyanobacterial dynamics and toxicity and human health risks. Data show an increasing cyanobacteria expansion and dominance in many environments. However there are still few information on the toxic species fitness, or on the effects of specific drivers on toxin production. Open research fields are related to new exposure scenario (cyanotoxins in water used for haemodialysis and in food supplements); to new patterns of co-exposure between cyanotoxins and algal toxins and/or anthropogenic chemicals; to dynamics affecting toxicity and production of different cyanotoxin variants under environmental stress; to the accumulation of cyanotoxins in the food web. In addition, many data gaps exist in the characterization of the toxicological profiles, especially about long term effects.


Toxicology Letters | 2013

The conjugation of microcystin-RR by human recombinant GSTs and hepatic cytosol

Franca M. Buratti; Simona Scardala; Enzo Funari; Emanuela Testai

Many cyanobacterial species can produce cyanotoxins, among which mycrocistins (MC) are a group of ≈100 congeners of hepatotoxic cyclic heptapeptides. MC-RR differs from MC-LR, the most studied congener only for one residue (arginine vs leucine), resulting in a ten-fold difference in the acute toxicity in mice. Although humans may be exposed to MC through several routes and kinetics appeared to be the major factor affecting congener-specific toxicity, little is known on MC metabolism. The accepted pathway for MC detoxication is GSH conjugation: here the MC-RR conjugation with GSH catalyzed by 5 recombinant human GSTs and human liver cytosol (HLC) has been characterized and appeared to be more efficient than MC-LR conjugation. The catalytic efficiency score is T1-1>A1-1≈P1-1>M1-1>A3-3 (0.161-0.056pmol GSMC-RR (μgproteinminμM)(-1)). In HLC the spontaneous reaction is favored vs the enzymatic one (ratio 3:1) at physiological GSH content. However, at low MC-RR concentrations, representative of repeated oral exposure, and low GSH content (down to 0.05mM), possibly associated to exposure to drugs or in patients affected by several pathologies, the relevance of the enzymatic reaction progressively increases, providing the predominant contribution to MC-RR detoxication.


Critical Reviews in Toxicology | 2016

Risk to human health associated with the environmental occurrence of cyanobacterial neurotoxic alkaloids anatoxins and saxitoxins.

Emanuela Testai; Simona Scardala; Susanna Vichi; Franca M. Buratti; Enzo Funari

Abstract Cyanobacteria are ubiquitous photosynthetic micro-organisms forming blooms and scums in surface water; among them some species can produce cyanotoxins giving rise to some concern for human health and animal life. To date, more than 65 cyanobacterial neurotoxins have been described, of which the most studied are the groups of anatoxins and saxitoxins (STXs), comprising many different variants. In freshwaters, the hepatotoxic microcystins represent the most frequently detected cyanotoxin: on this basis, it could appear that neurotoxins are less relevant, but the low frequency of detection may partially reflect an a priori choice of target analytes, the low method sensitivity and the lack of certified standards. Cyanobacterial neurotoxins target cholinergic synapses or voltage-gated ion channels, blocking skeletal and respiratory muscles, thus leading to death by respiratory failure. This review reports and analyzes the available literature data on environmental occurrence of cyanobacterial neurotoxic alkaloids, namely anatoxins and STXs, their biosynthesis, toxicology and epidemiology, derivation of guidance values and action limits. These data are used as the basis to assess the risk posed to human health, identify critical exposure scenarios and highlight the major data gaps and research needs.


Annali dell'Istituto Superiore di Sanità | 2010

Pesticides and their metabolites in selected Italian groundwater and surface water used for drinking

Luca Fava; Maria Antonietta Orrù; Simona Scardala; Elena Alonzo; Maristella Fardella; Caterina Strumia; Angiolo Martinelli; Sabrina Finocchiaro; Massimo Previtera; Alessandro Franchi; Piergiuseppe Calà; Mauro Dovis; Donatella Bartoli; Giuseppe Sartori; Lia Broglia; Enzo Funari

The control of groundwater and surface water quality in relation to the presence of pesticides and their metabolites is a rather complicated task. National and local authorities with the responsibility to guarantee an adequate quality of water cannot rely on international guidelines for monitoring activities. In a national project, forty-three pesticides and pesticide metabolites were selected on the basis of sale, monitoring and physical-chemical data, and investigated from some of the main Italian agricultural areas, susceptible to pesticide contamination. Twelve compounds were found in the tested water samples at levels exceeding the 0.1 µg/L European Union (EU) limit for drinking water (European Directive 98/83/EC). Maximum levels up to 0.62 were determined. Several water samples were characterized by the simultaneous occurrence of pesticides and their metabolites (up to ten). In some samples, the total concentration of pesticides and their metabolites was higher than the EU limit of 0.5 µg/L. Total triazine concentrations up to 1.02 µg/L were found. In all the cases the highest concentrations were well below the respective guideline values defined by the World Health Organization (WHO) for drinking water quality.


Journal of Coastal Research | 2011

Risk Management of Ostreopsis spp. Blooms Along Italian Coasts

Simona Scardala; Irene di Girolamo; Ernesto Fattorusso; Enzo Funari; Liana Gramaccioni; Rosella Bertolotto; Giancarlo Icardi; Daniela Mattei; Roberto Poletti; Emanuela Testai

ABSTRACT SCARDALA, S., DI GIROLAMO, I., FATTORUSSO, E., FUNARI, E., GRAMACCIONI, L., BERTOLOTTO, R., ICARDI, G., MATTEI, D., POLETTI, R and TESTAI, E., 2011. Risk Management of Ostreopsis spp. Blooms Along Italian Coasts. In: Micallef, A. (ed.), MCRR3-2010 Conference Proceedings, Journal of Coastal Research, Special Issue, No. 61, pp. 435–439. Grosseto, Tuscany, Italy, ISSN 0749-0208 In the last years Ostreopsis spp. blooms have been reported in several Italian coastal stretches, showing a clear trend towards their extension. In 2005, during summer, an O.ovata bloom occurring in the coastal area in front of Genoa was tentatively associated with some human effects. Symptoms like rhinorrea, cough, fever, conjunctivitis, bronchoconstriction with mild dyspnea, and wheezes were observed in people exposed to marine aerosols, possibly containing algal fragments and/or its toxin. With less severe symptoms, similar episodes have been sporadically recorded in other coastal areas. Up to these events, the institutional monitoring activities were focused only on the surveillance of planktonic microalgae whereas benthonic ones, such as Ostreopsis spp. were ignored. To fill this gap, in 2006 a group of experts was appointed by the Italian Ministry of Health with the task to elaborate specific guidelines to manage the possible riskassociated to Ostreopsis spp. blooms. After their formal endorsement, the guidelines were published in the official site of the Ministry and implemented by local structures. The guidelines identify surveillance activities to be carried out, subdividing them into the routine, alert and alarm phases. These guidelines are currently under revision in order to update the scientific background and refine the approach on the basis of the two-year application experience.


Environmental Science and Pollution Research | 2006

Leaching Potential of Some Phenylureas and Their Main Metabolites Through Laboratory Studies

Luca Fava; Maria Antonietta Orrù; Daniela Businelli; Simona Scardala; Enzo Funari

Collaboration


Dive into the Simona Scardala's collaboration.

Top Co-Authors

Avatar

Enzo Funari

Istituto Superiore di Sanità

View shared research outputs
Top Co-Authors

Avatar

Emanuela Testai

Istituto Superiore di Sanità

View shared research outputs
Top Co-Authors

Avatar

Susanna Vichi

Istituto Superiore di Sanità

View shared research outputs
Top Co-Authors

Avatar

Luca Fava

Istituto Superiore di Sanità

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Maura Manganelli

Istituto Superiore di Sanità

View shared research outputs
Top Co-Authors

Avatar

Franca M. Buratti

Istituto Superiore di Sanità

View shared research outputs
Top Co-Authors

Avatar

Mara Stefanelli

Istituto Superiore di Sanità

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Daniela Mattei

Istituto Superiore di Sanità

View shared research outputs
Researchain Logo
Decentralizing Knowledge