Siva Prasad Panda

Antidiabetic and antioxidant activity of Swietenia mahagoni in streptozotocin-induced diabetic rats

Context: Swietenia mahagoni L. Jacq. (Meliaceae) is a medium to large evergreen tree native to Southern Florida, Cuba, The Bahamas, Hispaniola, and Jamaica. Objective: To evaluate the antidiabetic and antioxidant potential of S. mahagoni bark. Materials and methods: In the present study, the antidiabetic activity of the methanol extract of S. mahagoni (MESM) bark in streptozotocin (STZ; 65 mg/kg body weight)-induced diabetic rats was evaluated. Glibenclamide (0.5 mg/kg; orally) was taken as the reference drug. The blood glucose levels and body weights were measured every 5th day over a period of 15 days. Antioxidant effects were assayed in diabetic rats by estimating thiobarbituric acid reactive substances (TBARS), glutathione (GSH), and catalase (CAT) levels. Results and discussion: Oral administration of MESM at the doses of 25 and 50 mg/kg b.w. resulted in a significant (p < 0.001) reduction in blood glucose levels in diabetic rats. Body weights were significantly (p < 0.001) reduced in STZ-induced diabetic rats when compared to normal rats, while the extract significantly restored body weight. The present study was further undertaken to evaluate the antioxidant activity of MESM in STZ-induced diabetic rats. Decreased levels of TBARS and increased levels of GSH and CAT activity indicated a reduction in free radical formation in tissues such as the liver and kidney of diabetic rats. Conclusion: These findings showed the significant hypoglycemic and antioxidant activity of the extract (MESM) in diabetic rats.

Hepatoprotective activity of Cyperus tegetum rhizome against paracetamol-induced liver damage in rats.

In present study the methanol extract of Cyperus tegetum rhizome (MECT) was evaluated for its effect on paracetamol-induced liver damage in Wistar rats. Serum biochemical parameters viz. serum glutamine oxaloacetate transaminase (SGOT), serum glutamine pyruvate transaminase (SGPT), serum alkaline phosphatase (ALP), total serum protein, total bilirubin content and liver biochemical parameters such as thiobarbituric acid reactive substances (TBARS) and reduced glutathione content were estimated. Biochemical and histopathological observations indicated that MECT had remarkable hepatoprotective effect against paracetamol-induced liver damage in rats.

Colorimetric Analysis of Raltegravir in Pharmaceutical Formulation and Human Biological Fluids through Oxidative Coupling Reaction using 3-Methyl-2-Benzothiazolinone Hydrazone Reagent

A unique and speedy colorimetric method has been developed and validated for the estimation of Raltegravir in pharmaceutical formulation and human biological fluids using 3-methyl-2-benzothiazolinone hydrazone (MBTH) reagent. The projected technique is based on oxidative coupling reaction of MBTH reagent with Raltegravir by using ferric chloride solution and HCl to produce bluish green chromogen at 572nm. The linearity was established in the range of 80-220 µg/mL with regression equation 0.004x+0.007 having r2=0.999. The method shows good precision and ruggedness with accuracy in between 99.85%-100.43% with % RSD less than 2%. The optical characteristics and various statistical reports were reported successfully and there was no interference of any excipients and reagents in this method. The method which was depicted in this research work was successfully applied and the results are compared with the reference standards and satisfactory validated results were obtained.