Sl Johnston
Imperial College London
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Sl Johnston.
European Respiratory Journal | 2003
B Abraham; Jm Anto; E. Barreiro; Ehd Bel; Giovanni Bonsignore; Jean Bousquet; J Castellsague; Pascal Chanez; F Cibella; G Cuttitta; Barbro Dahlén; S-E Dahlén; N Drews; Ratko Djukanovic; Lm Fabbri; Gert Folkerts; Mina Gaga; C Gratziou; G Guerrera; S. T. Holgate; Ph Howarth; Sl Johnston; F. Kanniess; Johan Kips; Ham Kerstjens; Maria Kumlin; H Magnussen; Fp Nijkamp; N Papageorgiou; Alberto Papi
Since severe asthma is a poorly understood, major health problem, 12 clinical specialist centres in nine European countries formed a European Network For Understanding Mechanisms Of Severe Asthma (ENFUMOSA). In a cross-sectional observational study, a total of 163 subjects with severe asthma were compared with 158 subjects whose asthma was controlled by low doses of inhaled corticosteroids (median dose of beclomethasone equivalents 666u2005µg). Despite being treated with higher doses of inhaled corticosteroids (median dose 1773u2005µg) and for a third of the severe asthmatics also being treated with regular, oral-steroid therapy (median daily dose 19u2005mg), the subjects with severe asthma met the inclusion criteria. The criteria required subjects to have undergone at least one asthma exacerbation in the past year requiring oral steroid treatment. Females dominated the severe asthma group (female/male ratio 4.4:1 versus 1.6:1 in the controlled asthmatics), and compared with controlled asthmatics, they had a predominantly neutrophilic inflammation (sputum neutrophils, 36 versus 28%) and evidence of ongoing mediator release but less atopy. From these findings and other physiological and clinical data reported in this paper, it is suggested that severe asthma might be a different form of asthma rather than an increase in asthma symptoms. The findings prompt for longitudinal studies and interventions to define the mechanisms in severe asthma.
European Respiratory Journal | 2002
Peter Wark; Sl Johnston; I. Moric; Jodie L. Simpson; Michael J. Hensley; Peter G. Gibson
Acute exacerbations of asthma are frequently caused by viral infections, but the inflammatory mechanisms in virus-induced asthma are poorly understood. The aim of the present study was to determine whether viral infection in acute asthma was associated with increased sputum neutrophil degranulation and increased cellular lysis and whether these changes are related to clinical severity. Adults (n=49) presenting to the emergency department with acute asthma were examined for infection by means of sputum direct-fluorescence antigen detection, sputum culture, and sputum polymerase chain reaction for Mycoplasma, Chlamydia and Legionella pneumophila, and all common respiratory viruses. Subjects infected with one of these agents were classed as having an infective exacerbation. Spirometry and sputum induction were performed on presentation and 4–5u2005weeks later. Thirty-seven subjects (76%) had virus infection and acute asthma. Those with virus infection had increased sputum neutrophils (p<0.05) and increased neutrophil elastase (p<0.05), this was related to increased elevated sputum lactate dehydrogenase (LDH). Subjects with noninfective asthma had an increase in the proportion of sputum eosinophils. Both groups had elevated sputum eosinophil cationic protein (ECP) concentrations. Higher levels of sputum LDH and ECP were associated with a longer hospital stay. Virus infection and acute asthma is associated with neutrophilic inflammation, cell lysis and more severe clinical disease.
Thorax | 2002
Nikolaos G. Papadopoulos; Luminita A. Stanciu; Alberto Papi; Stephen T. Holgate; Sl Johnston
Background: Rhinoviruses (RVs) are the most frequent precipitants of the common cold and asthma exacerbations, but little is known about the immune response to these viruses and its potential implications in the pathogenesis of asthma. Methods: Peripheral blood mononuclear cells (PBMC) from patients with atopic asthma and normal subjects were exposed to live or inactivated RV preparations. Levels of interferon (IFN)γ and interleukins IL-12, IL-10, IL-4, IL-5 and IL-13 were evaluated in the culture supernatants with specific immunoassays. Results: Exposure of PBMC to RVs induced the production of IFNγ, IL-12, IL-10, and IL-13. Cells from asthmatic subjects produced significantly lower levels of IFNγ and IL-12 and higher levels of IL-10 than normal subjects. IL-4 was induced only in the asthmatic group, while the IFNγ/IL-4 ratio was more than three times lower in the asthmatic group. Conclusions: This evidence suggests that the immune response to RVs is not uniquely of a type 1 phenotype, as previously suggested. The type 1 response is defective in atopic asthmatic individuals, with a shift towards a type 2 phenotype in a way similar, but not identical, to their aberrant response to allergens. A defective type 1 immune response to RVs may be implicated in the pathogenesis of virus induced exacerbations of asthma.
Clinical & Experimental Allergy | 2001
Nikolaos G. Papadopoulos; Alberto Papi; J. Meyer; Luminita A. Stanciu; Sundeep Salvi; Stephen T. Holgate; Sl Johnston
Background Human rhinoviruses (RVs) are the most common precipitants of asthma exacerbations. RV infection of bronchial epithelium results in local airway inflammation inducing eosinophil recruitment and activation. Induction of eosinophil chemoattractants could represent a central mechanism, as well as a prime target for intervention.
Allergy | 2007
Nikolaos G. Papadopoulos; Paraskevi Xepapadaki; Patrick Mallia; Guy Brusselle; Jean-Baptiste Watelet; M. Xatzipsalti; G. Foteinos; C. M. Van Drunen; W. J. Fokkens; C. D'Ambrosio; Sergio Bonini; Apostolos Bossios; Jan Lötvall; P. Van Cauwenberge; Stephen T. Holgate; G. W. Canonica; Andrzej Szczeklik; Gernot Rohde; J. Kimpen; A. Pitkaranta; Mika J. Mäkelä; P. Chanez; Johannes Ring; Sl Johnston
Viral infections of the respiratory tract are the most common precipitants of acute asthma exacerbations. Exacerbations are only poorly responsive to current asthma therapies and new approaches to therapy are needed. Viruses, most frequently human rhinoviruses (RV), infect the airway epithelium, generate local and systemic immune responses, as well as neural responses, inducing inflammation and airway hyperresponsiveness. Using in vitro and in vivo experimental models the role of various proinflammatory or anti‐inflammatory mediators, antiviral responses and molecular pathways that lead from infection to symptoms has been partly unravelled. In particular, mechanisms of susceptibility to viral infection have been identified and the bronchial epithelium appeared to be a key player. Nevertheless, additional understanding of the integration between the diverse elements of the antiviral response, especially in the context of allergic airway inflammation, as well as the interactions between viral infections and other stimuli that affect airway inflammation and responsiveness may lead to novel strategies in treating and/or preventing asthma exacerbations. This review presents the current knowledge and highlights areas in need of further research.
European Respiratory Journal | 2002
Peter Wark; Sl Johnston; Jodie L. Simpson; Michael J. Hensley; Peter G. Gibson
Infection with Chlamydia pneumoniae can trigger acute asthma and is associated with severe chronic asthma. The aim of the present study was to examine the relationship between airway inflammation and serological response to C. pneumoniae in acute severe asthma. Subjects (n=54) were recruited within 4u2005h of presentation to the emergency department with an acute exacerbation of asthma. Clinical history taking, sputum induction (0.9% saline), spirometry and acute and convalescent serology for C. pneumoniae immunoglobulins A and G were performed. At presentation, 47% of subjects had antibodies directed against C. pneumoniae, and 38% (20) demonstrated an increase in C. pneumoniae antibody levels, with 15 demonstrating a rise in immunoglobulin A concentration. C. pneumoniae responders exhibited significantly higher sputum neutrophil levels (4.6×106 cells·mL−1) compared to nonresponders (1.2×106 cells·mL−1, p=0.02) and elevated sputum eosinophil cationic protein concentration (3,981 versus 1,122u2005ng·mL−1, p=0.02). An acute antibody response to Chlamydia pneumoniae is common in exacerbations of asthma. The serological features suggest that Chlamydia pneumoniae reactivation may trigger neutrophilic airway inflammation in acute asthma.
Clinical & Experimental Allergy | 2002
S.-H. Cho; L. A. Stanciu; T. Begishivili; P. J. Bates; Stephen T. Holgate; Sl Johnston
Background Increased production of IL‐4 and IL‐5 and decreased production of IFN‐γ by CD4+ T cells has been implicated in asthma pathogenesis. However, CD8+ T cells also produce type 1 and type 2 cytokines and the relative roles of CD4+ and CD8+ T cell cytokine production in asthma have not been previously studied.
European Respiratory Journal | 2010
C.A. Hewson; J.J. Haas; Nathan W. Bartlett; Simon D. Message; V. Laza-Stanca; Tatiana Kebadze; Gaetano Caramori; Jie Zhu; M.R. Edbrooke; Luminita A. Stanciu; Onn Min Kon; Alberto Papi; Peter K. Jeffery; Michael R. Edwards; Sl Johnston
Rhinovirus (RV) infections are the major cause of asthma exacerbations, the major cause of morbidity and mortality in asthma. MUC5AC is the major mucin produced by bronchial epithelial cells. Whether RV infection upregulates MUC5AC in vivo is unknown and the molecular mechanisms involved are incompletely understood. We investigated RV induction of MUC5AC in vivo and in vitro to identify targets for development of new therapies for asthma exacerbations. RV infection increased MUC5AC release in normal and asthmatic volunteers experimentally infected with RV-16, and in asthmatic, but not normal, subjects, this was related to virus load. Bronchial epithelial cells were confirmed a source of MUC5AC in vivo. RV induction of MUC5AC in bronchial epithelial cells in vitro occurred via nuclear factor-&kgr;B-dependent induction of matrix metalloproteinase-mediated transforming growth factor-&agr; release, thereby activating an epidermal growth factor receptor-dependent cascade culminating, via mitogen-activated protein kinase activation, in specificity protein-1 transactivation of the MUC5AC promoter. RV induction of MUC5AC may be an important mechanism in RV-induced asthma exacerbations in vivo. Revealing the complex serial signalling cascade involved identifies targets for development of pharmacologic intervention to treat mucus hypersecretion in RV-induced illness.
Current Pharmaceutical Design | 2007
Patrick Mallia; Gaetano Caramori; A. Pandit; Sl Johnston; Alberto Papi
Asthma and chronic obstructive pulmonary disease (COPD) are the 2 most prevalent chronic airway diseases. Much of the morbidity, mortality and health care costs of the diseases are associated with acute exacerbations, which are episodes of increased symptoms and airflow obstruction. Over the last decade evidence has emerged implicating virus respiratory tract infections as a major cause of exacerbations of both asthma and COPD. Current therapies are not very effective in the prevention or treatment of virus-induced exacerbations and exacerbations are therefore a major unmet medical need. The development of new and novel treatments requires a better understanding of the molecular and cellular mechanisms linking virus infection with exacerbations of asthma and COPD. This article provides an overview of current knowledge regarding the mechanisms of virus-induced exacerbations in both asthma and COPD. It will also review existing treatments and future treatments that are in advanced stages of development.
Clinical & Experimental Allergy | 2002
Nikolaos G. Papadopoulos; Luminita A. Stanciu; Alberto Papi; Stephen T. Holgate; Sl Johnston
Background Rhinovirus (RV) infection is the commonest trigger of acute asthma exacerbations; however, the immune response to these viruses and any potential implications in the mechanisms leading to asthma exacerbations are not well understood.