Sławomir Keszka

ASIAN PANGASIIDS-AN EMERGING PROBLEM FOR EUROPEAN INLAND WATERS? SYSTEMATIC AND PARASITOLOGICAL ASPECTS

Some species of the pangasiid catfishes are economically very important and have a large culture potential. They have been successfully bred and cultured in Thailand and Vietnam (Lerssutthichawal et al. 1999), from where they are exported to the European and American markets, processed mainly into fillets. Those fish, commonly referred to as “panga”, have hitherto been unknown to Europe and the USA. The most valuable catfish species are: sutchi catfish, Pangasianodon hypophthalmus (Sauvage, 1878), and basa, Pangasius bocourti Sauvage, 1880, which can be raised in the river-based cages or earth-constructed ponds. Their hybrids are also of high economical value (Hung et al. 2003). Pangasiids were also experimentally cultured in Puerto Rico during 2003–2004. They were successfully reproduced and cultured with results similar to those achieved in Asian aquaculture (McGee 2005). Moreover, Pangasius sp. is regarded as a new species for aquaculture (however not new to market or consumers) in central and eastern Europe (Varadi 2008). It is anticipated that in the next 5 years worldwide aquaculture production of Pangasius sp. could be similar to that achieved with tilapia and salmon (McGee 2005). ACTA ICHTHYOLOGICA ET PISCATORIA (2009) 39 (2): 131–138 DOI: 10.3750/AIP2009.39.2.08

Genetic identifiability of selected populations of Indian mackerel, Rastrelliger kanagurta (Actinopterygii: Perciformes: Scombridae)—Celfish Project—Part 1

Genetic identification of food ingredients is an important method that enables the traceability and confirmation of authenticity of fish products. With regard to common cases where valuable fish species are replaced by lowvalue equivalents it is necessary to provide reliable methods which will precisely identify fish species that are valuable for economy. Such methods help to eliminate counterfeit products from the market but also provide tools that might be used by governmental units which control food safety, including custom services. Population identification by molecular methods also provides inforACTA ICHTHYOLOGICA ET PISCATORIA (2014) 44 (2): 145–152 DOI: 10.3750/AIP2014.44.2.08

Genetic variability in European populations of Coregonus lavaretus (L.): an assessment based on mitochondrial ND-1 gene haplotypes

Genetic variability in European populations of Coregonus lavaretus (L.): an assessment based on mitochondrial ND-1 gene haplotypes The genetic variability of whitefish, Coregonus lavaretus (L.), was studied based on 114 individuals from nine populations inhabiting Polish lakes, including the Szczecin Lagoon, and from one population each from lakes in Austria and Switzerland. Differences within and among populations were assessed with mitochondrial ND-1 gene sequences that were PCR amplified and digested with ten restriction enzymes. The ten composite haplotypes obtained were sequenced and analyzed with MEGA4 software. The highest intrapopulation variability was noted in the whitefish populations inhabiting lakes Ińsko, Miedwie, Marianowo, Wisola, Śremskie, Morzycko, the Szczecin Lagoon, and Lake Lucerne, which possessed from two to five composite haplotypes. In contrast, populations inhabiting lakes Woświn, Czarne, and Traunsee were fixed for the most common haplotype H2. Zróżnicowanie genetyczne europejskiej populacji Coregonus lavaretus (L.): ocena na podstawie mitochondrialnego genu ND-1 Zróżnicowanie genetyczne 9 populacji siei pospolitej (Coregonus lavaretus, L.) określono w grupie 114 osobników pozyskanych ze zbiorników wodnych Polski, Szwajcarii oraz Austrii. Analizę przeprowadzono w oparciu o technikę PCR-RFLP zastosowaną względem mitochondrialnego genu ND-1, którego sekwencję poddano trawieniu 10 enzymom restrykcyjnym. Na tej podstawie otrzymano dziesięć haplotypów, których sekwencje reprezentatywne zostały poddane sekwencjonowaniu oraz analizie z wykorzystaniem programu MEGA4. Najwyższe zróżnicowanie międzypopulacyjne określono dla populacji siei zamieszkujących jeziora Ińsko, Miedwie, Marianowo, Wisola, Śremskie, Morzycko, Zalew Szczeciński oraz szwajcarskie jezioro Lucerne. Zawierały się ona w obrębie haplotypów od 2 do 5. Dla porównania osobniki z jezior Woświn, Czarne (DPN) oraz Traunsee (Austria) były zaklasyfikowane do pojedynczego haplotypu H2.

Development of the method for identification of selected populations of torpedo scad, Megalaspis cordyla (Linnaeus, 1758), using microsatellite DNA analyses. CELFISH project – Part 4

Catch and consumption of torpedo scad (Megalaspis cordyla) over the western Indian Ocean, but also the western Pacific from Japan to Australia is constantly increasing. Taking into account the degree of exploitation and missing information on the population structure of torpedo scad stocks it is crucial to provide population data. The analysis included individuals obtained in 2012 and 2013 from local markets in Madagascar, Tanzania, Vietnam and Cambodia and after successful DNA extraction fragment of the nuclear rhodopsin gene (RH1) and 9 microsatellite regions (SSRs) were amplified and analysed. Based on the obtained results it was found that there was no 100% overlap between the compared RH1 sequences and those from GenBank. In the case of the studied SSRs, the results allowed the initial characterisation and assessment of the genetic diversity of populations. Moreover, population assignment test distinguished the studied populations into two geographically distant subpopulations.

Microsatellite DNA-based genetic traceability of two populations of splendid alfonsino, Beryx splendens (Actinopterygii: Beryciformes: Berycidae)—Project CELFISH—Part 2

Background. The study is a contribution to Project CELFISH which involves genetic identifi cation of populations of fi sh species presenting a particular economic importance or having a potential to be used in the so-called commercial substitutions. The EU fi sh trade has been showing a distinct trend of more and more fi sh species previously unknown to consumers being placed on the market. Molecular assays have become the only way with which to verify the reliability of exporters. This paper is aimed at pinpointing genetic markers with which to label and differentiate between two populations of splendid alfonsino, Beryx splendens Lowe, 1834, a species highly attractive to consumers in Asia and Oceania due to the meat taste and low fat content. Material and methods. DNA was isolated from fragments of fi ns collected at local markets in Japan (MJ) (n = 10) and New Zealand (MNZ) (n = 18). The rhodopsin gene (RH1) fragment and 16 microsatellite DNA fragments (SSR) were analysed in all the individuals. The sequences obtained were processed using the BioEdit and BLAST software, whereas SSR data were processed with the GeAlEX analysis package. Results. The BioEdit software-aided comparison of MJ and MNZ nucleotide sequences of the rhodopsin gene fragments were identical and showed 100% agreement with the alfonsino sequence deposited under access number DQ197832. The preliminary analysis of SSR markers showed all the loci analysed in both populations to be polymorphic, and when randomly selected specimens were assigned to the original populations. The affi nity test correctly identifi ed the provenance of all those specimens. Conclusion. The results obtained constitute a tool for molecular differentiation between alfonsino populations collected in the FAO 81 (New Zealand) and FAO 71 (Japan) areas for the purpose of catch quota control and for checking the agreement between the label declaration and the actual product.

Studies on endangered and rare non-commercial fish species recorded in the Pomeranian Bay (southern Baltic Sea) in 2010–2013

This paper presents the results of studies on endangered and rare non-commercial fish species (Spinachia spinachia, Nerophis ophidion, Syngnathus typhle, Agonus cataphractus, Pholis gunnellus, Enchelyopus cimbrius, Cyclopterus lumpus) and one lamprey species (Lampetra fluviatilis), recorded as bycatch during monitoring surveys in 2010–2013 in the Pomeranian Bay. Two species were observed for the first time in the Pomeranian Bay: A. cataphractus and E. cimbrius. Descriptions of parasite fauna are provided for C. lumpus and E. cimbrius, which were infected with four pathogenic species from Neomonada, Digenea, Nematoda, and Acanthocephala. Almost all parasite species were new in the hosts examined.