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Dive into the research topics where Sofie Tanghe is active.

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Featured researches published by Sofie Tanghe.


Theriogenology | 2003

Cumulus contributions during bovine fertilization in vitro

Sofie Tanghe; Ann Van Soom; Jalil Mehrzad; Dominiek Maes; Luc Duchateau; Aart de Kruif

A mandatory step in performing micromanipulation techniques, studying sperm-oocyte interactions and evaluating morphological aspects of oocyte quality is the removal of cumulus cells from oocytes or zygotes at various stages. In cattle, cumulus removal shortly before fertilization in vitro strongly decreases sperm penetration rates. This study was conducted to evaluate the function of the cumulus oophorus during bovine fertilization in vitro. The importance of cumulus secretions during IVF was investigated by inseminating cumulus-denuded oocytes (CDOs) in fertilization medium supplemented with individual cumulus secretions, such as progesterone or hyaluronic acid. None of these substances increased the fertilization rate of CDOs. However, fertilizing CDOs in cumulus-conditioned medium or on a cumulus monolayer partially restored the reduction in fertilization rate (P<0.05). The fertilization rate of CDOs inseminated on a cumulus monolayer further increased when physical contact between the gametes and the monolayer was prevented by fertilizing them inside a culture plate insert placed on the monolayer (P<0.05). Finally, the importance of reactive oxygen species (ROS) generation and O(2) concentration during IVF was studied. Luminol-dependent chemiluminescence revealed a higher ROS load in conditioned medium of cumulus-enclosed oocytes (CEOs) than in that of CDOs after sperm-oocyte co-incubation (P<0.05). Furthermore, lowering the external O(2) concentration from 20 to 5% decreased the fertilization rate of both CEOs and CDOs, but had a higher impact on CEOs (P<0.05). In conclusion, this study provides evidence that the cumulus oophorus benefits the fertilizing ability of penetrating spermatozoa by creating a complex microenvironment of both cumulus secretions and metabolic products around the oocyte. Gap junctional communication between the oocyte and corona cells as well as sperm trapping by the cumulus oophorus seem to be essential factors in supporting fertilization.


Molecular Reproduction and Development | 2002

Minireview: Functions of the cumulus oophorus during oocyte maturation, ovulation, and fertilization.

Sofie Tanghe; Ann Van Soom; Hans Nauwynck; Marc Coryn; Aart de Kruif


Theriogenology | 2005

New techniques for the assessment of canine semen quality: a review.

Tom Rijsselaere; A. Van Soom; Sofie Tanghe; Marc Coryn; D. Maes; A. de Kruif


Reproduction in Domestic Animals | 2002

Function of the cumulus oophorus before and during mammalian fertilization.

A. Van Soom; Sofie Tanghe; I De Pauw; D. Maes; A. de Kruif


Journal of Arid Environments | 2005

Impact of water harvesting techniques on soil and water conservation: a case study on a micro catchment in southeastern Tunisia

Wouter Schiettecatte; M. Ouessar; Donald Gabriëls; Sofie Tanghe; S Heirman; F Abdelli


Reproduction in Domestic Animals | 2002

Assessment of Different Sperm Quality Parameters to Predict in vitro Fertility of Bulls

Sofie Tanghe; A. Van Soom; V. Sterckx; D. Maes; A. de Kruif


Molecular Reproduction and Development | 2004

Inhibition of bovine sperm-oocyte fusion by the p-aminophenyl derivative of D-mannose.

Sofie Tanghe; A. Van Soom; Luc Duchateau; A. de Kruif


Reproduction | 2005

Inhibition of bovine sperm–zona binding by bovine herpesvirus-1

Sofie Tanghe; Geert Vanroose; A. Van Soom; Luc Duchateau; M.T. Ysebaert; P Kerkhofs; Etienne Thiry; S van Drunen Littel-van den Hurk; P Van Oostveldt; Hans Nauwynck


Workshop ‘Gamete function and interaction leading towards embryo development’ | 2001

Effect of oviduct vesicles on in vitro penetration rate of cumulus-denuded oocytes in cattle

Sofie Tanghe; Ann Van Soom; Dominiek Maes; Aart de Kruif


Theriogenology | 2001

Progesterone alone is not sufficient to mimic the beneficial effect of a cumulus monolayer on the penetration rates of cumulus-free oocytes

Sofie Tanghe; Ann Van Soom; Marc Coryn; Hans Laevens; Aart de Kruif

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