Soledad Muniategui

Variation of apparent ethanol content of unspoiled northwestern Spanish honeys during storage

Abstract This paper describes, for the first time, the variation of apparent ethanol content during storage of 33 honey samples. Moisture content was determined by measuring refractive index at 20°C. Apparent ethanol content was determined in a double cuvette, according to a modification of the Boehringer–Mannheim enzymatic method. Four different types of apparent ethanol evolution were observed: constant increment, increment followed by decrease, values oscillation and constant decrease.

Direct Enzymatic Analysis of Glycerol in Honey: a Simplified Method

The glycerol content of honey has been determined using a Boehringer Mannheim enzymatic method simplified for this purpose. The solution of honey was employed directly, without clarification and without addition of NaOH solution. With these conditions, there were no observed interference effects. It is necessary to use a 1.5 ml micro-cuvette. The enzymatic determination was measured spectrophotometrically at 365 nm using the enzymes in quadruple the quantities recommended by the supplier. The method combines precision, good recovery, sensitivity, simplicity and low cost.