Sónia Duarte

Dietary-driven variation effects on the symbiotic flagellate protist communities of the subterranean termite Reticulitermes grassei Clément

The ability of subterranean termites to digest lignocellulose relies not only on their digestive tract physiology, but also on the symbiotic relationships established with flagellate protists and bacteria. The objective of this work was to test the possible effect of different cellulose‐based diets on the community structure (species richness and other diversity metrics) of the flagellate protists of the subterranean termite Reticulitermes grassei. Termites belonging to the same colony were subjected to six different diets (natural diet, maritime pine wood, European beech, thermally modified European beech, cellulose powder and starvation), and their flagellate protist community was evaluated after the trials.

OPTIMIZATION OF TERMITE IN-GROUND MONITORING STATIONS: AN EVALUATION TRIAL

Subterranean termites are serious pests of wood in service in much of the world. One of the most popular techniques for monitoring and controlling termites is the use of in-ground monitoring stations. Different cellulosic matrices were evaluated in terms of mass loss, moisture content variation and termite presence, accounting for spatial coordinates and monitoring station conditional variables, including: type of cellulosic matrix, matrix treatment and meteorological conditions, during one year in field conditions, both in Portugal and in the USA. A multivariate redundancy analysis was performed resulting in 60.4% of data variability being explained by the variables considered in this analysis. Spatial variables were responsible for the highest amount of variance observed in the response variables monitored for the termite monitoring stations, followed by the type of cellulosic matrix, from which cellulose and Hevea brasiliensis were the most influential variables. The optimization of termite in-ground monitoring stations should be performed through correct evaluation of termite feeding preferences and decay resistance, in order to choose an adequate bait matrix and a proper bait design. The termite species biology and the geographical location where control programs will be applied should also be taken into account.

Symbiotic flagellate protists as cryptic drivers of adaptation and invasiveness of the subterranean termite Reticulitermes grassei Clément

Abstract Changes in flagellate protist communities of subterranean termite Reticulitermes grassei across different locations were evaluated following four predictions: (i) Rural endemic (Portugal mainland) termite populations will exhibit high diversity of symbionts; (ii) invasive urban populations (Horta city, Faial island, Azores), on the contrary, will exhibit lower diversity of symbionts, showing high similarity of symbiont assemblages through environmental filtering; (iii) recent historical colonization of isolated regions—as the case of islands—will imply a loss of symbiont diversity; and (iv) island isolation will trigger a change in colony breeding structure toward a less aggressive behavior. Symbiont flagellate protist communities were morphologically identified, and species richness and relative abundances, as well as biodiversity indices, were used to compare symbiotic communities in colonies from urban and rural environments and between island invasive and mainland endemic populations. To evaluate prediction on the impact of isolation (iv), aggression tests were performed among termites comprising island invasive and mainland endemic populations. A core group of flagellates and secondary facultative symbionts was identified. Termites from rural environments showed, in the majority of observed colonies, more diverse and abundant protist communities, probably confirming prediction (i). Corroborating prediction (ii), the two least diverse communities belong to termites captured inside urban areas. The Azorean invasive termite colonies had more diverse protist communities than expected and prediction (iii) which was not verified within this study. Termites from mainland populations showed a high level of aggressiveness between neighboring colonies, in contrast to the invasive colonies from Horta city, which were not aggressive to neighbors according to prediction (iv). The symbiotic flagellate community of R. grassei showed the ability to change in a way that might be consistent with adaptation to available conditions, possibly contributing to optimization of the colonization of new habitats and spreading of its distribution area, highlighting R. grassei potential as an invasive species.