Sônia Oliveira Lima

Effect of Bovine Type-I Collagen-Based Films Containing Red Propolis on Dermal Wound Healing in Rodent Model

El propoleo es un producto apiterapico ampliamente usado en medicina natural, que presenta diversas propiedades biologicas, como actividad bactericida y anti-inflamatoria, pero pocos informes han examinado la variedad brasilena del propoleo rojo. Peliculas de colageno han sido empleadas para mejorar la cicatrizacion de heridas y en algunos estudios se ha investigado el efecto de los productos naturales incorporados en estas peliculas. Asi el objetivo de este estudio fue analizar el efecto de las peliculas a base de colageno que contienen propoleo rojo en la cicatrizacion de heridas. Heridas realizadas en el dorso de ratas fueron revestidas con peliculas a base de colageno (G2) y con peliculas a base de colageno con propoleo rojo (G3), heridas sin revestimiento fueron utilizadas como control (G1). Despues de 3, 7 y 14 dias, las heridas fueron evaluadas microscopicamente. A los 7 dias G3 mostro un cambio del perfil de la respuesta inflamatoria de aguda a cronica, con aumento de la diferenciacion de las celulas plasmaticas, sustitucion de fibras de colageno tipo III por tipo I con mejora de la organizacion arquitectonica de estas fibras. Ademas, una ligera tendencia a una mejor epitelizacion se verifico en G3, pero no fue estadisticamente significativa. Tambien a los 7 dias, el conteo de microvasos fue mayor en G1 que en G2 (p=0,000) y G3 (p=0,001), pero similar entre los ultimos grupos (p=0,15), mientras que el perimetro de los microvasos fue mayor en G1 que en G2 (p=0,000) y G3 (p=0,002), pero equivalente en G2 y G3 (p=0,175) en 14 dias. En conclusion, la incorporacion de la variedad brasilena de propoleo rojo en peliculas a base de colageno fue capaz de mejorar la cicatrizacion de la herida, probablemente por la modulacion de la dinamica de la evolucion inflamatoria y del proceso de deposito de colageno.

Evaluation of anxiety and depression prevalence in patients with primary severe hyperhidrosis

BACKGROUND Primary hyperhidrosis (PH) can lead to mood changes due to the inconveniences it causes. OBJECTIVE This study aimed to examine the existence of anxiety and depression in patients with severe primary hyperhidrosis who sought treatment at a medical office. METHODS The questionnaire Hospital Anxiety and Depression Scale was used for 197 individuals, in addition to the chi square test and Fisher exact test, p <0.05. RESULTS There was an increased prevalence of anxiety (49.6%) but not of depression (11.2%) among patients with PH, with no link to gender, age or amount of affected areas. Palmar and plantar primary hyperhidrosis were the most frequent but when associated with the presence of anxiety, the most frequent were the axillary (p = 0.02) and craniofacial (p = 0.02) forms. There was an association between patients with depression and anxiety (p = 0.001). CONCLUSIONS the involvement of Primary hyperhidrosis was responsible for a higher prevalence of anxiety than that described among the general population and patients with other chronic diseases. Depression had a low prevalence rate, while mild and moderate forms were the most common and frequently associated with anxiety. The degree of anxiety was higher in mild and moderate types than in the severe form.

Research of primary hyperhidrosis in students of medicine of the State of Sergipe, Brazil.

BACKGROUND Hyperhidrosis or excessive sweat production occurs at 2.9-9% of the population. OBJECTIVE To estimate the prevalence and disorders due to primary hyperhidrosis (HP) in medicine students in the state of Sergipe. METHODS Cross-sectional study using individual interviews. RESULTS Hyperhidrosis was found in 14.76% of subjects, the most affected regions were palmar, plantar and axillary, causing prejudice in daily activities. Family history occurred in 45% and 22.72% was diagnosed by a physician. CONCLUSION The prevalence of hyperhidrosis in medicine students of Sergipe was high, with strong family and a small portion of diagnoses made by medical professionals.

Avaliação epidemiológica das vítimas de trauma abdominal submetidas ao tratamento cirúrgico

OBJECTIVEnTo evaluate the profile and outcome of victims of abdominal injuries who underwent laparotomy in the Emergency Hospital of the state of Sergipe (HUSE).nnnMETHODSnThis was an observational, descriptive longitudinal study with prospective approach, through interviews of 100 patients with abdominal trauma who underwent surgery and evaluation of their medical records. The study period was from September to November of 2011 in the area of trauma care of the HUSE.nnnRESULTSnthe most affected individuals were male, mulattos, aged 25-49 years, with low education, single, Catholic, with an income of 1-2 minimum wages. There was a predominance of trauma in the urban areas, at night and on weekends. The most frequent cause of trauma was the attempted of murder associated with the use of alcohol and illicit drugs, and the most frequent mechanism, stabbing. The pain was the most frequent symptom. The most affected region was the upper abdomen and liver was the most affected organ. The hospital stay averaged 4-10 days. Most patients were discharged without sequelae. There were 2 deaths.nnnCONCLUSIONnIn the HUSE, the association of abdominal trauma with men under the influence of alcohol and illegal drugs was striking, reflecting the context of interpersonal violence in current society. Despite the magnitude of the traumas, the outcome was satisfactory, although deaths, occurred, demonstrating the importance of keeping a trained surgical team in emergency hospitals.

Enhancement of liver regeneration by the association of Hyptis pectinata with laser therapy.

Since new molecules that normally would accelerate regeneration can also be potentialized by light, the use of new substances combined with laser therapy seems to be a natural type of experiment. Therefore, the purpose of this study was to assess the effects of Hyptis pectinata leaves on liver regeneration after partial hepatectomy (PH) associated with laser therapy. Twenty-four rats were divided into four groups—PH(control), PHL (laser therapy), PH200 (200 mg/kg of Hyptis pectinata), and PHL200 (200 mg/kg of the plant and laser)—which were submitted to 67% hepatectomy. Laser treatment consisted of focusing the light on the remaining liver after hepatectomy. The data analyzed were serum levels of aminotransferases, liver regeneration, and mitochondrial function. Group PH200 showed a statistically significant decrease in AST levels, and PHL200 disclosed an augmentation in ALT levels. The liver regeneration index was significantly increased in group PHL200. Concerning liver mitochondrial respiratory assay, groups PH200 and PHL200 showed lower state 3 levels than groups PH and PHL. Group PHL showed an increase in state 4 levels and a reduction in membrane potential and RCR. The present study shows that the association of the aqueous extract of Hyptis pectinata leaves at 200 mg/kg with intraoperative laser therapy can stimulate liver regeneration and cause a reduction in liver mitochondrial respiratory function without altering its phosphorylative activity.

Efeito do omeprazol e do pantoprazol sobre a regeneração hepática após hepatectomia parcial em ratos

PURPOSE: To assess the effects of omeprazole and pantoprazole on liver regeneration after partial hepatectomy. METHODS: Fifty eight male Wistar rats were divided into 4 groups: SHAM, HP, PANTO and OMEP Groups. They were submitted to 67% partial hepatectomy (HP, PANTO and OMEP Groups) or laparotomy (SHAM Group). Their livers were removed 32 and 56 hours after the operation. Then, the animals were sacrificed. In all groups, the substances (saline solution, omeprazole and pantoprazole) were injected once daily from the moment they were operated on until the time of sacrifice. RESULTS: In SHAM Group the mitotic index was not significant. Thirty two hours after hepatectomy, the mitosis index was 1.2 ± 1.09 in HP Group, 1.2 ± 1.6 in OMEP Group and 2.6 ± 3.2 in PANTO Group. Concerning the 56-hour analysis, the values were 1.6 ± 0.89 in HP Group, 2 ± 1.8 in OMEP Group and 2.6 ± 0.54 in PANTO Group. These results were not statistically significant. CONCLUSION: Omeprazole and pantoprazole, proton pump inhibitors (H+, K+-ATPase), do not cause any change on liver regeneration 32 and 56 hours after 67% partial hepatectomy in rats.

Estado vacinal e conhecimento dos profissionais de saúde sobre hepatite B em um hospital público do nordeste brasileiro

ABSTRACT OBJECTIVE: To analyze the vaccination status and knowledge on hepatitis B virus of healthcare workers (HW) from a public hospital. METHODS: Cross-sectional study, based on a written questionnaire answered by HW, which included biomedical personnel, dentists, nurses, physiotherapists, physicians, nurse assistants, and lab technicians. They informed about their age, profession, immunization status and knowledge on hepatitis B. Chi-square test was used, considering p < 0.05 as statistically significant. RESULTS: During four months in 2007, questionnaires were filled by 861 professionals from the main public emergency hospital in Sergipe State, Northeastern region of Brazil. There was a statistically significant relationship between educational level and HWs previous knowledge on hepatitis B. As for the vaccination status, 67% of the HW reported being fully immunized, while 21.6% had not taken all the vaccines, 6.2% was unaware of their status, and 5.2% was not vaccinated. Among university level HW 75.3% was fully immunized, while 64,5% of high-school-level technicians was. CONCLUSION: University degree HW showed a greater knowledge on the subject when compared to technicians, results that should motivate managers in healthcare to implement continuing education for their personnel as well as research about this topic.

EFEITOS DO EXTRATO AQUOSO DA HYPTIS PECTINATA SOBRE A REGENERAÇÃO HEPÁTICA APÓS HEPATECTOMIA PARCIAL DE 70%: RESULTADOS PRELIMINARES

The use of medicinal plants has strongly increased by the world population. The objetive of this study is to assess the effects of the aqueous extract of Hyptis pectinata leaves, popularly known as sambacaita or canudinho, on liver regeneration after 70% partial hepatectomy. Twenty four rats were divided into 4 groups: group OS, submitted to sham operation and oral administration of distilled water during 4 days; group OSD200, also submitted to sham operation and ingestion of 200 mg of extract/Kg of animal for the same period of time; group HP, which underwent 70% hepatectomy after 4 days of distilled water administration; and group HPD200, which underwent 70% hepatectomy after 4 days of oral administration of 200 mg of extract/Kg of animal. Alkaline phosphatase, total bilirubin and the serum level of aminotransferases were measured and state III of mitochondrial respiratory activity was assessed. Group OSD200, when compared to OS, presented significant decrease of alcaline fosfatase. Group HPD200, when compared to HP, showed statistically significant decrease of AST level and state III respiratory process.

Solid-pseudopapillary tumor of pancreas in a young woman: a case report and literature review.

Solid-pseudopapillary tumor of pancreas is a rare neoplasm which occurs pre-dominantely in young females. This tumor generally is asymptomatic or minimally symptomatic and some imaging tests are useful for its diagnosis. We report a 17 years-old woman with dyspeptic symptoms submitted to an abdominal ultrasound (US), that revealed a hypoechogenic mass between the liver, right kidney and pancreas. Computer tomography (CT) scan showed a hypodense and heterogeneous mass on pancreas head. The patient underwent elective resection of the mass and the histopathology was consistent with a solid-pseudopapillary tumor of pancreas. Immunohistochemical positivity for NSE (neuron-specific enolase), progesterone receptor, alfa-1-antitrypsin, vimentin, AE1/AE3, and negativity for synaptophysin and chromogranin A confirmed the diagnosis. After four years of clinical follow-up, the patient remains in a good general condition without signs of tumor recurrence.El tumor solido pseudopapilar de pancreas es una neoplasia poco frecuente, que ocurre predominantemente en mujeres jovenes. Este tumor generalmente es asintomatico o minimamente sintomatico y las imagenes son utiles para su diagnostico. Presentamos una mujer de 17 anos de edad con dispepsia que fue sometida a una ecografia abdominal que revelo una masa tumoral hipoecogenica entre el higado, el rinon derecho y el pancreas. La tomografia computarizada mostro una masa hipodensa y heterogenea en la cabeza del pancreas. La paciente fue sometida a reseccion electiva de la masa y la histopatologia fue consistente con el tumor solido pseudopapilar de pancreas. La inmunohistoquimica fue positiva para NSE (enolasa neuronal especifica), receptor de progesterona, alfa-1-antitripsina, vimentina, AE1/ AE3, y negativa para sinaptofisina y cromogranina A, lo que confirmo el diagnostico. Despues de cuatro anos seguimiento clinico, la paciente permanece en buen estado general sin signos de recurrencia del tumor.

Does the aging process significantly modify the Mean Heart Rate

Background The Mean Heart Rate (MHR) tends to decrease with age. When adjusted for gender and diseases, the magnitude of this effect is unclear. Objective To analyze the MHR in a stratified sample of active and functionally independent individuals. Methods A total of 1,172 patients aged ≥ 40 years underwent Holter monitoring and were stratified by age group: 1 = 40-49, 2 = 50-59, 3 = 60-69, 4 = 70-79, 5 = ≥ 80 years. The MHR was evaluated according to age and gender, adjusted for Hypertension (SAH), dyslipidemia and non-insulin dependent diabetes mellitus (NIDDM). Several models of ANOVA, correlation and linear regression were employed. A two-tailed p value <0.05 was considered significant (95% CI). Results The MHR tended to decrease with the age range: 1 = 77.20 ± 7.10; 2 = 76.66 ± 7.07; 3 = 74.02 ± 7.46; 4 = 72.93 ± 7.35; 5 = 73.41 ± 7.98 (p < 0.001). Women showed a correlation with higher MHR (p <0.001). In the ANOVA and regression models, age and gender were predictors (p < 0.001). However, R2 and ETA2 < 0.10, as well as discrete standardized beta coefficients indicated reduced effect. Dyslipidemia, hypertension and DM did not influence the findings. Conclusion The MHR decreased with age. Women had higher values of MHR, regardless of the age group. Correlations between MHR and age or gender, albeit significant, showed the effect magnitude had little statistical relevance. The prevalence of SAH, dyslipidemia and diabetes mellitus did not influence the results.