Sophi Damayanti

Synthesis and Characterization Molecularly Imprinted Polymers for Analysis of Dimethylamylamine Using Acrylamide as Monomer Functional

A selective separation techniques with Molecularly Imprinted Polymer (MIP) for High-Performance Liquid Chromatography (HPLC) has been developed for the assay of Dimethylamylamine (DMAA) doping compounds. Molecular imprinted polymer (MIP) is a technique to produce a polymer having the cavity due to the disposal of the templates, in which the cavity serves to recognize the molecules of the same size, structure, chemical and physical properties. The selectivity and affinity of the templates itself will increase, while the concentration value is increasing. MIP is made by DMAA as template, acrylamide as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross linking, azobisisobutyronitrile (AIBN) as the initiator and chloroform as a porogen solvent; using bulk method. The aim of research are conduct the MIP for the DMAA compound analysis, then the formed MIP is characterized by using Fourier Transform Infra Red (FTIR) and Scanning Electron Microscopy (SEM) to find out the polymer complexes formed and the morphological form of the MIP. The MIP formed then was analyzed by using High-Performance Liquid Chromatography (HPLC) to know the amount of the DMAA, the adsorption capacity, and the adsorption condition found in the MIP. The result of analysis on the content of DMAA in the MIP by using UV-Vis Spectrophotometer is 1.957 mg. Scanning Electron Microscopy (SEM) shows that the MIP has irregular and rough morphological structure; while the NIP has irregular morphology structures and smooth surfaces shape

CONSUMPTION STUDY OF SUPPLEMENT ON MEMBER OF GYMS IN BANDUNG AND QUALITATIVE ANALYSIS OF STEROID IN SELECTED SUPPLEMENT

Objective: Inflammation is body reactions in response to tissue injury and infection. In 2011, non-steroidal anti-inflammatory drug (NSAID) was the highest demand drug in Indonesia. However, long-term treatment using NSAID can cause several side effects to cardiovascular and digestive system. This research aimed to investigate anti-inflammatory properties of binahong leaves (Anredera cordifolia) and pegagan herbs (Centella asiatica). Methods: Ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs was evaluated for its anti-inflammatory properties using human red blood cell (RBC) – membrane stabilization assay. The extract concentrations used in this study was 100, 200, 400, and 800-ppm, and apigenin and asiaticoside concentration were 1, 2, 3, 4, 5, 6, 10, and 100 ppm. Diclofenac natrium (DN) was used as a standard drug. Results: The results showed that A. cordifolia extract (ACE) alone, C. asiatica extract (CAE) alone, and the combination of ACE and CAE could inhibit the hemolysis of RBC in hypotonic solution. The optimum concentration for ACE alone was 100 ppm; for CAE alone was 400 ppm; and for the combination of ACE and CAE was 50 ppm and 50 ppm, respectively. Apigenin and asiaticoside in concentration of 1-10 ppm showed more than 97% inhibition of hemolysis. DN as a standard drug showed optimum inhibition at concentration of 400 ppm. Conclusion: The ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs showed anti-inflammatory activity, both as a single treatment or as combinations, and apigenin and asiaticoside were responsible for anti-inflammatory activity in C. asiatica. Keywords: Anti-inflammation, Human red blood cell – membrane stabilization, Anredera cordifolia, Centella asiatica.

Validation of spectrophotometric method for lactulose assay in syrup preparation

Lactulose is a synthetic disaccharide widely used in food and pharmaceutical fields. In the pharmaceutical field, lactulose is used as osmotic laxative in a syrup dosage form. This research was aimed to validate the spectrophotometric method to determine the levels of lactulose in syrup preparation and the commercial sample. Lactulose is hydrolyzed by hydrochloric acid to form fructose and galactose. The fructose was reacted with resorcinol reagent, forming compounds that give absorption peak at 485 nm. Analytical methods was validated, hereafter lactulose content in syrup preparation were determined. The calibration curve was linear in the range of 30-100 μg/mL with a correlation coefficient (r) of 0.9996, coefficient of variance (Vxo) of 1.1 %, limit of detection of 2.32 μg/mL, and limit of quantitation of 7.04 μg/mL. The result of accuracy test for the lactulose assay in the syrup preparation showed recoveries of 96.6 to 100.8 %. Repeatability test of lactulose assay in standard solution of lactulose a...

Interactions of Porphyrin-Acridine Hybrids to DNA Duplexes and Quadruplex: In Silico Study

The binding modes of cationic porphyrin hybrids to DNA has been studied in a previous study. In the present research, cationic porphyrinacridine hybrids bearing meso-substituted pyridine, imidazole, and pyrazole rings were investigated for their interaction with DNA. AutoDock Vina was used to dock 11 compounds to four different DNA duplexes, dodecamers d(CGCAAATTTGCG)2 (PDB code: 102D) and d(CGCGAATTCGCG)2 (PDB code: 1PRP), and hexamers d(CGATCG)2 (PDB code: 1Z3F) and d(TGATCA)2 (PDB code: 182D), as well as a human telomeric DNA quadruplex (PDB code: 1KF1). The binding mode and affinity of each compound were then compared to that of meso-tetrakis(4-methylpyridiniumyl)porphyrin (TMPyP). The hybrid compounds interacted with the DNA duplexes through intercalation and groove binding, while the interaction with DNA quadruplex was strictly stacking. Porphyrin-acridine hybrids with two meso-substituted diazolium rings exhibited higher affinity towards both DNA duplex and quadruplex than that of monoand tri-substituted derivatives. Bis-H2PyP-2AC resulted in the highest affinity towards DNAs representing minor groove binding, with binding free energies of –12.3 and –13.8 kcal/mol towards 102D and 1PRP, respectively, and bis-H2ImP-2AC docked well with quadruplex DNA 1KF1 with a binding free energy of –11.6 kcal/mol.

O-desmethylquinine as a Cyclooxygenase-2 (COX-2) Inhibitors Using AutoDock Vina

Computational approach was employed to evaluate the biological activity of novel cyclooxygenase-2 COX-2 inhibitor, O-desmethylquinine, in comparison to quinine as common inhibitor which can also be used an agent of antipyretic, antimalaria, analgesic and antiinflamation. The molecular models of the compound were constructed and optimized with the density function theory with at the B3LYP/6-31G (d,p) level using Gaussian 09 program. Molecular docking studies of the compounds were done to obtain the COX-2 complex structures and their binding energies were analyzed using the AutoDock Vina. The results of docking of the two ligands were comparable and cannot be differentiated from the energy scoring function with AutoDock Vina.