Sophie P. Laut

Adaptive-optics optical coherence tomography for high-resolution and high-speed 3D retinal in vivo imaging

We have combined Fourier-domain optical coherence tomography (FD-OCT) with a closed-loop adaptive optics (AO) system using a Hartmann-Shack wavefront sensor and a bimorph deformable mirror. The adaptive optics system measures and corrects the wavefront aberration of the human eye for improved lateral resolution (~4 μm) of retinal images, while maintaining the high axial resolution (~6 μm) of stand alone OCT. The AO-OCT instrument enables the three-dimensional (3D) visualization of different retinal structures in vivo with high 3D resolution (4×4×6 μm). Using this system, we have demonstrated the ability to image microscopic blood vessels and the cone photoreceptor mosaic.

Characterization for vision science applications of a bimorph deformable mirror using phase-shifting interferometry

The wave front corrector is one of the three key elements in adaptive optics, along with the wave front sensor and the control computer. Low cost, compact deformable mirrors are increasingly available. We have tested the AOptix bimorph deformable mirror, originally developed for ultra-high bandwidth laser communication systems, to determine its suitability for vision science applications, where cornea and lens introduce optical aberrations. Measurements of the dynamic response of the mirror to a step input were obtained using a commercial Laser Doppler Vibrometer (LDV). A computer-controlled Twyman-Green interferometer was constructed to allow the surface height of the deformable mirror to be measured using Phase-Shifting Interferometry as a function of various control voltages. A simple open-loop control method was used to compute the control voltages required to generate aberration mode shapes described by the Zernike polynomials. Using this method, the ability of the deformable mirror to generate each mode shape was characterized by measuring the maximum amplitude and RMS error of each Zernike mode shape up to the fifth radial order. The maximum deformation amplitude was found to diminish with the square of the radial order of the Zernike mode, with a measured deformation of 8 microns and 1.5 microns achieved at the second-order and fifth-order Zernike modes, respectively. This deformation amplitude appears to be sufficient to allow the mirror to correct for aberrations up to the fifth order in the human eye.

Exposure time dependence of image quality in high-speed retinal in vivo Fourier domain OCT

We built a Fourier domain optical coherence tomography (FD-OCT) system using a line scan CCD camera that allows real time data display and acquisition. This instrument is able to produce 2D B-scans as well as 3D data sets with human subjects in vivo in clinical settings. In this paper we analyze the influence of varying exposure times of the CCD detector on image quality. Sensitivity values derived from theoretical predictions have been compared with measurements (obtained with mirrors and neutral density filters placed in both interferometer arms). The results of these experiments, discussion about differences between sensitivity values, potential sources of discrepancies, and recommendations for optimal exposure times will be described in this paper. A short discussion of observed artifacts as well as possible ways to remove them is presented. The influence of relative retinal position with respect to reference mirror position will also be described.

Bimorph deformable mirror: an appropriate wavefront corrector for retinal imaging?

The purpose of this study was to evaluate the performance of a bimorph deformable mirror from AOptix, inserted into an adaptive optics system designed for in-vivo retinal imaging at high resolution. We wanted to determine its suitability as a wavefront corrector for vision science and ophthalmological instrumentation. We presented results obtained in a closed-loop system, and compared them with previous open-loop performance measurements. Our goal was to obtain precise wavefront reconstruction with rapid convergence of the control algorithm. The quality of the reconstruction was expressed in terms of root-mean-squared wavefront residual error (RMS), and number of frames required to perform compensation. Our instrument used a Hartmann-Shack sensor for the wavefront measurements. We also determined the precision and ability of the deformable mirror to compensate the most common types of aberrations present in the human eye (defocus, cylinder, astigmatism and coma), and the quality of its correction, in terms of maximum amplitude of the corrected wavefront. In addition to wavefront correction, we had also used the closed-loop system to generate an arbitrary aberration pattern by entering the desired Hartmann-Shack centroid locations as input to the AO controller. These centroid locations were computed in Matlab for a user-defined aberration pattern, allowing us to test the ability of the DM to generate and compensate for various aberrations. We conclude that this device, in combination with another DM based on Micro-Electro Mechanical Systems (MEMS) technology, may provide better compensation of the higher-order ocular wavefront aberrations of the human eye

OPTICAL CHARACTERIZATION OF A BIMORPH DEFORMABLE MIRROR

This paper reports the results of interferometric characterization of a bimorph deformable mirror (DM) designed for use in an adaptive optics (AO) system. The natural frequencies of this DM were measured up to 20 kHz using both a custom stroboscopic phase-shifting interferometer as well as a commercial Laser Doppler Vibrometer (LDV). Interferometric measurements of the DM surface profile were analyzed by fitting the surface with mode-shapes predicted using classical plate theory for an elastically-supported disk. The measured natural frequencies were found to be in good agreement with the predictions of the theoretical model.Copyright

Scanning laser ophthalmoscope design with adaptive optics

A design for a high-resolution scanning instrument is presented for in vivo imaging of the human eye at the cellular scale. This system combines adaptive optics technology with a scanning laser ophthalmoscope (SLO) to image structures with high lateral (~2 μm) resolution. In this system, the ocular wavefront aberrations that reduce the resolution of conventional SLOs are detected by a Hartmann-Shack wavefront sensor, and compensated with two deformable mirrors in a closed-loop for dynamic correction and feedback control. A laser beam is scanned across the retina and the reflected light is captured by a photodiode, yielding a two-dimensional image of the retina at any depth. The quantity of back-scattered light from the retina is small (0.001% of reflection) and requires the elimination of all parasite reflections. As an in vivo measurement, faint cellular reflections must be detected with a low-energy source, a supraluminescent laser diode, and with brief exposures to avoid artifacts from eye movements. The current design attempts to optimize trade-offs between improved wavefront measurement and compensation of the optical aberrations by fractioning the light coming to the wavefront sensor, better sensitivity by increasing the input light energy or the exposure time and the response speed of the system. This instrument design is expected to provide sufficient resolution for visualizing photoreceptors and ganglion cells, and therefore, may be useful in diagnosing and monitoring the progression of retinal pathologies such as glaucoma or aged-related macular degeneration.

Adaptive-Optics High-Resolution and High-Speed Retinal in vivo Fourier-Domain OCT

We have combined a Fourier-domain optical coherence tomograph (OCT) with a closed-loop Adaptive Optics (AO) system. The AO-OCT instrument has been then used for in vivo retinal imaging.

Adaptive-optics optical coherence tomography for high-resolution and high-speed in vivo retinal imaging

We have combined Fourier-domain optical coherence tomography (OCT) with a closed-loop Adaptive Optics (AO) system. The AO-OCT instrument has been used for in vivo retinal imaging. High-lateral resolution of our AO-OCT system allows visualization of the microscopic retinal structures not accessible by standard OCT instruments.