Sorin Răpuntean

Preliminary Data on the In Vitro Effect of Copper-Based Compounds on Aerobic Bacterial Microflora Isolated from Sheep Footrot

Contagious sheep footrot is an infectious disease with chronic evolution, usually enzootic, characterized by necrotic or necrotic-purulent inflammation of the soft tissues of the hoof, which causes partial or total detachment of the horn. Cu-based products represented by (I) 1-hydroxyquinoline + Cu on 5% hydroxyapatite, (II) 2 Hydroxyquinoline + Cu + NO3 on 5% Hydroxyapatite and (III) 3 Nitrohydroxyquinoline + Cu on 5% Hydroxyapatite were conditioned in aqueous suspension in a volume of 100 ml, pH 6,0 – 6,4. The products were placed into wells, 20 μL/well, along with 0.5 % copper sulphate solution (Cu control) and Enroxil and Oxitetracycline (control for antibiotics). The results demonstrated that 7 of the samples (58.33 %) are sensitive to the product I, 6 samples (50%) to the product III, 4 samples (33.33 %) to the product 2 and the 0.5% Copper sulphate solution only has a bacteriostatic effect. In comparison to the two antibiotics, 9 samples (75 %) are sensitive to enrofloxacin and only 5 samples (41.66 %) to oxytetracycline. Regarding the associations of microorganisms in the samples with resistance, we have found that these are represented by the most common germs of genus Bacillus, Corynebacterium and Gram negative bacteria, and rarer associations with Micrococcus and Staphylococcus.

Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

Abstract The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.