Soykan Dinc

Risk Factors for Breast Cancer in Turkish Women with Early Pregnancies and Long-lasting Lactation

A hospital-based case-control study was carried out among 504 women with breast cancer and 610 controls to analyse the risk factors for breast cancer in Turkey. Adjusted odds ratios (ORs) and 95% confidence intervals (CIs) for each risk factor were obtained from logistic regression analysis. Risk factors for breast cancer were found to be long-term lactation ( S 5 years versus never OR 0.31, 95% CI 0.12-0.79), young age at menarche (<15 years versus S 15 OR 1.72, 95% CI 1.30-2.28), late age at first full-term pregnancy ( S 30 versus <20 OR 2.86, 95% CI 1.32-6.21), oral contraceptive use (ever versus never OR 1.51, 95% CI 1.10-2.08), positive family history (positive versus negative OR 2.81, 95% CI 1.35-5.82), and menstrual irregularity (yes versus no OR 1.61, 95% CI 1.05-2.49). The results of the present study will lead to a better understanding of the risk factors for breast cancer in a developing country.

Locally applied granulocyte-macrophage colony-stimulating factor improves the impaired bowel anastomoses in rats with long-term corticosteroid treatment

Inflammation is an essential component of the first phase of anastomotic wound healing, and it is suppressed by corticosteroids. The anti-inflammatory effect of corticosteroids is largely responsible for the impairment of wound healing in bowel anastomosis. It has been reported that local application of granulocyte-macrophage colony-stimulating factor (GM-CSF) improves the healing process in dermal wounds. In the present study, we investigated the effects of locally injected GM-CSF on anastomotic wound healing in long-term corticosteroid treated rats. Eighty male Sprague-Dawley rats were randomized into four groups. 1: control, 2: steroid, 3: steroid + local GM-CSF, 4: steroid + systemic GM-CSF. In groups 2, 3, and 4, methylprednisolone (5 mg/kg) was injected IM daily for 14 days. After 14 days, following anesthesia and laparotomy, colonic anastomosis was performed 3 cm away from the peritoneal reflection. In group 3, 50 mg GM-CSF was injected into the perianastomotic area. In group 4, 50 mg GM-CSF was applied subcutaneously. On postoperative day 3, repeat laparotomies were performed and bursting pressures, hydroxyproline levels, and histopathology examinations were studied. The mean values of bursting pressures and hydroxyproline levels in group 3, treated with steroid + local GM-CSF, were significantly higher than that of the group 2 and group 4 values. In the histopathology examination, the mean score of group 3 was significantly higher than that of groups 2 and 4. Our study indicates that local application of GM-CSF significantly improves the impaired anastomotic wound healing in rats treated with long-term corticosteroid.

Granulocyte Macrophage-Colony Stimulating Factor Improves Impaired Anastomotic Wound Healing in Rats Treated with Intraperitoneal Mitomycin-C

PurposeIntraperitoneal chemotherapy (IPCT) delivers higher local concentrations of cytotoxic drugs than intravenous (IV) chemotherapy, but it can adversely affect the healing of intestinal anastomoses if given in the early postoperative period. Intestinal anastomotic leakage is a serious surgical complication. Experimental and clinical studies have shown that the local administration of granulocyte macrophage-colony stimulating factor (GM-CSF) improves would healing. Therefore, we evaluated the effects of locally applied GM-CSF on anastomotic wound healing in rats treated with intraperitoneal mitomycin-C immediately after surgery.MethodsWe performed colon anastomoses in albino rats, which were then divided into three treatment groups. Group A was a control group that received no treatment, Group B was given intraperitoneal mitomycin-C postoperatively, and Group C was given intraperitoneal mitomycin-C with a local injection of GM-CSF postoperatively. We measured bursting pressures and hydroxyproline content, and histologically examined the resected anastomoses on postoperative day (POD) 3.ResultsAnastomotic healing was impaired after intraperitoneal mitomycin-C, but this was overcome by the injection of GM-CSF into the perianastomotic area.ConclusionLocal GM-CSF administration counteracts the detrimental effects of intraperitoneal mitomycin-C treatment on intestinal anastomoses in rats.

Local Granulocyte-Macrophage Colony-Stimulating Factor Improves Incisional Wound Healing in Adriamycin-Treated Rats

PurposeNeoadjuvant treatment is often given for locally advanced malignancies; however, clinical and experimental studies have shown that some chemotherapeutic agents impair wound healing. It has been reported that granulocyte-macrophage colony-stimulating factor (GM-CSF) applied locally improves dermal wound healing. Thus, we investigated the effects of locally injected GM-CSF on abdominal wounds impaired by adriamycin, a widely used chemotherapeutic agent.MethodsWe divided 120 female Sprague-Dawley rats into five treatment groups of 24 rats. Group 1 received saline 8 mg/kg intravenously (i.v.) + laparotomy 14 days later (control); group 2 received 8 mg/kg i.v. adriamycin + laparotomy 14 days later; group 3 received adriamycin 8 mg/kg i.v. + laparotomy + local GM-CSF 50 µg 14 days later; group 4 received saline 8 mg/kg i.v. + laparotomy + local GM-CSF 50 µg 14 days later; and group 5 received adriamycin 8 mg/kg i.v. + laparotomy + systemic GM-CSF 50 µg 14 days later. Sutures were removed on postoperative day (POD) 7 in all five groups, and the abdominal bursting pressures were measured and recorded. Tissue samples were taken from the incision line for histopathological evaluation and hydroxyproline content measurement.ResultsThe bursting pressure was significantly lower in groups 2 and 5 than in groups 1, 3, and 4. The hydroxyproline content and histopathological findings supported this result.ConclusionThe local injection of GM-CSF improved impaired wound healing in adriamycin-treated rats.

Prognostic Significance of Apex Axillary Invasion for Locoregional Recurrence and Effect of Postmastectomy Radiotherapy on Overall Survival in Node-Positive Breast Cancer Patients

Postmastectomy irradiation substantially reduces the risk of locoregional recurrences (LRR) of breast carcinoma. However, debates continue on the prognostic factors for radiotherapy and the effect of radiotherapy on overall survival. The present study was undertaken to investigate the prognostic significance of level III positive nodes, along with the other factors for LRR, and to evaluate the effect of postmastectomy radiotherapy on overall survival among node-positive breast carcinoma treated systemically. Data from 549 consecutive node-positive breast cancer patients who underwent modified radical mastectomy and received adjuvant systemic therapy were studied retrospectively. Prognostic factors for LRR and the effect of postmastectomy radiotherapy on overall survival were analyzed. Survival curves were generated by the Kaplan-Meier method, and multivariate analysis was performed by the Cox proportional hazard model. The 5-year locoregional recurrence rate is 7%. Apical invasion was found to be an independent prognostic factor for LRR (HR 2.6, CI 1.29–5.35) along with a finding of 4 or more positive nodes and T3 tumor. Adjuvant radiotherapy decreased LRR and improved survival significantly. Apical invasion, 4 or more positive axillary lymph nodes, and T3 tumor are the predictors of LRR, and patients with these adverse factors are candidates for adjuvant radiotherapy. Postmastectomy radiotherapy improves overall survival.

Optimal timing for surgery after adriamycin treatment in rats.

PurposeTo determine the optimal timing of surgery after adriamycin treatment, we investigated the time-related effect of adriamycin on wound healing over a long period.MethodsWe divided 119 female Sprague-Dawley rats into seven treatment groups. Group 1 was subjected to laparatomy only. All the other groups were given 8 mg/kg adriamycin intravenously followed by laparotomy on the same day (group 2), 7 days later (group 3), 14 days later (group 4), 21 days later (group 5), 28 days later (group 6), or 35 days later (group 7). On postoperative day 7, the sutures were removed, abdominal bursting pressure was measured, and tissue samples were taken for histopathological evaluation and analysis of hydro-xyproline content.ResultsBursting pressures were significantly lower in groups 3, 4, 5, and 6 than in group 1. The hydroxyproline content and histopathological evaluation supported these findings.ConclusionsOur results showed that the optimal timing for surgery after adriamycin treatment is before the 7th day or after the 35th day. If surgery is performed between these days, there is a high risk of impaired wound healing.

Temporal variation in the recovery from impairment in adriamycin-induced wound healing in rats

Background An adriamycin-induced impairment of wound healing has been demonstrated experimentally in rats. The purpose of this study is to investigate a possible temporal variation in recovery from the impairment of wound healing caused by adriamycin administration. Methods The subjects were 120 female Spraque-Dawley rats. They were divided into eight groups, undergoing adriamycin administration (8 mg/kg, i.v.) at 9 a.m. or 9 p.m. on day 0 and laparotomy on day 0, 7, 14 or 21. Blast pressures were recorded after the incision line had been opened, and tissue samples were kept at -30°C for later measurement of hydroxyproline levels. Results Adriamycin treatment in rats at 9 p.m. resulted in significantly lower blast pressure levels than treatment at 9 a.m. between days 7 and 21, indicating a lag effect of healing time in wounded tissues. However the decreased hydroxyproline levels were not changed at these days and sessions. Conclusion It is concluded that adriamycin-induced impairment of wound healing in adult female rats exhibits nycthemeral variation.

Methylene Blue Inhibits the Inflammatory Process of the Acetic Acid-Induced Colitis in Rat Colonic Mucosa

Inflammatory bowel disease is a serious health problem. Although it has been widely investigated, treatment of inflammatory bowel diseases currently remains as a challenging clinical problem. Over production of nitric oxide has been demonstrated to cause tissue damage and inflammation. In this study, the effect of methylene blue (MB), a well-known inhibitor of nitric oxide synthesis, was investigated in acetic acid (AA)-induced colitis model in Sprague-Dawley rats. Eighty male rats randomized into 4 groups (control, control MB, colitis, colitis + MB). AA was applied to groups 3 and 4. MB was added into group 2 and 4. Three days later, animals were sacrificed and 8 cm distal colonic segment resected and the specimens are examined using macroscopical, histological, and biochemical methods. The results of the macroscopic and microscopic examination showed that in group 4 the mucosal damage and inflammation score significantly lower than group 3. Increased intestinal permeability in acetic acid-administered group was significantly reversed by MB application. Myeloperoxidase activity and malondialdehyde levels increased significantly, while superoxide dismutase and catalase activities were suppressed after AA-administration. These biochemical parameters were reversed in MB-treated group. Administration of acetic acid resulted in increased levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, total nitrite/nitrate levels and nitric oxide synthase activity. These biochemical alterations were significantly reversed by MB application also. In conclusion, our results indicate that MB decreases the level of nitric oxide and decreases inflammation in acetic acid-induced colitis.

Sıçanlarda iskemik deri fleplerinde oral β-glukanın etkisi

GIRIŞ Flep cerrahisi sonrasi dokularda gorulen nekroz gunumuzde en onemli problemlerden biri olmaya devam etmektedir. Bu problemi cozmeye yonelik bircok medikal ve cerrahi calisma halen devam etmektedir (1,2). Random iskemik deri fleplerinde nekroza; venoz donus eksikligi, iskemi ve oksijenin neden oldugu bilinmektedir. Bu bilgi cercevesinde deneysel calismalar yapilmis ve sempatolitikler, vazodilatatorler, antikoagulanlar, prostoglandin inhibitorleri, serbest oksijen radikal tutuculari gibi bircok farmakolojik ajan flebi iskemiye karsi korumak icin kullanilmistir (3,4). Deneysel olarak bazi ajanlarin faydalari saptanmis fakat klinik pratikte bu ajanlarin kullanimi ile ilgili belirli fikir birligine varilamamistir. Glukan Saccharomyces cerevisiae‘nin duvarindan izole edilen dogal polisakkarid yapisinda bir maddedir (5). β-1.3 glukan oral yoldan etkili nontoksik guclu bir immunostimulandir. 1980’li yillarin sonlarinda bu materyalin makrofajlarin yuzeyinde var olan spesifik reseptorlere etki ettigi tanimlanmis, makrofajlarin β glukan reseptorleri oldugu ve bu reseptorlere baglanmanin makrofaj aktivasyonuna yol actigi bildirilmistir (6-8). Sicanlarda yurutulen deneysel calismalar glukan uygulamasinin granulosit ve makrofaj uretimini arttirdigini dusundurmektedir. β-Glukanin antibakteriyal, antifungal, antiviral, antitumoral, antienflamatuar etkileri ve makrofaj aktivasyonuna etkileri bildirilmistir (9-13). Bu bilgilerin isiginda yapilan korele invivo calismalarda glukanin major torasik ve gastrointestinal ARAŞTIRMA YAZISI

The effects of oral glucan on ischemic skin flap in rats

GIRIŞ Flep cerrahisi sonrasi dokularda gorulen nekroz gunumuzde en onemli problemlerden biri olmaya devam etmektedir. Bu problemi cozmeye yonelik bircok medikal ve cerrahi calisma halen devam etmektedir (1,2). Random iskemik deri fleplerinde nekroza; venoz donus eksikligi, iskemi ve oksijenin neden oldugu bilinmektedir. Bu bilgi cercevesinde deneysel calismalar yapilmis ve sempatolitikler, vazodilatatorler, antikoagulanlar, prostoglandin inhibitorleri, serbest oksijen radikal tutuculari gibi bircok farmakolojik ajan flebi iskemiye karsi korumak icin kullanilmistir (3,4). Deneysel olarak bazi ajanlarin faydalari saptanmis fakat klinik pratikte bu ajanlarin kullanimi ile ilgili belirli fikir birligine varilamamistir. Glukan Saccharomyces cerevisiae‘nin duvarindan izole edilen dogal polisakkarid yapisinda bir maddedir (5). β-1.3 glukan oral yoldan etkili nontoksik guclu bir immunostimulandir. 1980’li yillarin sonlarinda bu materyalin makrofajlarin yuzeyinde var olan spesifik reseptorlere etki ettigi tanimlanmis, makrofajlarin β glukan reseptorleri oldugu ve bu reseptorlere baglanmanin makrofaj aktivasyonuna yol actigi bildirilmistir (6-8). Sicanlarda yurutulen deneysel calismalar glukan uygulamasinin granulosit ve makrofaj uretimini arttirdigini dusundurmektedir. β-Glukanin antibakteriyal, antifungal, antiviral, antitumoral, antienflamatuar etkileri ve makrofaj aktivasyonuna etkileri bildirilmistir (9-13). Bu bilgilerin isiginda yapilan korele invivo calismalarda glukanin major torasik ve gastrointestinal ARAŞTIRMA YAZISI