Sri Atun

Identification and antioxidant activity test of bioactive compound produced from ethanol extract of temukunci (Boesenbergia rotunda)

The aims of this study are to identify and to analyze antioxidant activity of bioactive compounds in ethanol extract of temukunci (Boesenbergia rotunda) rhizome. This research method was carried out by maceration of 3 kg dryed powder of B. rotunda in ethanol. The extract was concentrated using a vacuum evaporator. White crystals obtained from ethanol extract further recrystallized to produce as much as 80 mg of pure compound. Identification of pure compound was obtained using UV-Vis, IR, and 1H-NMR. The antioxidant activity of ethanol extracts, pure compounds, and ascorbic acid (positive control) were obtained using DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The UV-Vis spectra showed λmax 287.40 and 214.20 nm, the IR spectra showed absorption C=C aromatic group at 1571.66 cm−1, C=O carbonyl at 1639.37 cm−1 and CO at 1153.35 cm−1. 1H-NMR spectra showed a group monosubtituted benzene, benzene tetrasubstituted, proton of hydroxyl group, proton of methoxyl group, one proton oxyalkyl, and two protons alkyl. The spectroscopic data showed the compound is pinostrobin (5-hydroxy-7-metoxyiflavanone). The antioxidant activity (IC50) of the ethanol extracts from B. rotunda rhizome, pinostrobin, and ascorbic acid (positive control) were 92.6; 62.84; and 3.7 µg/mL repectively. The study showed that the ethanol extract of B. rotunda rhizome and bioactive compounds have high antioxidant activity, but their activity lower than ascorbic acid.The aims of this study are to identify and to analyze antioxidant activity of bioactive compounds in ethanol extract of temukunci (Boesenbergia rotunda) rhizome. This research method was carried out by maceration of 3 kg dryed powder of B. rotunda in ethanol. The extract was concentrated using a vacuum evaporator. White crystals obtained from ethanol extract further recrystallized to produce as much as 80 mg of pure compound. Identification of pure compound was obtained using UV-Vis, IR, and 1H-NMR. The antioxidant activity of ethanol extracts, pure compounds, and ascorbic acid (positive control) were obtained using DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The UV-Vis spectra showed λmax 287.40 and 214.20 nm, the IR spectra showed absorption C=C aromatic group at 1571.66 cm−1, C=O carbonyl at 1639.37 cm−1 and CO at 1153.35 cm−1. 1H-NMR spectra showed a group monosubtituted benzene, benzene tetrasubstituted, proton of hydroxyl group, proton of methoxyl group, one proton oxyalkyl, and two protons alkyl. ...

Synthesis of Nanoparticles Produced by Ethanol Extract of Boesenbergia rotunda Rhizome Loaded with Chitosan and Alginic Acid and its Biological Activity test

Introduction: B. rotunda used as a traditional medicine to treat illnesses such as rheumatism, muscle pain, febrifuge, gout, gastrointestinal disorders, flatulence, carminative, stomach ache, dyspepsia, and peptic ulcer. To enhance the stability, solubility and activity of the extract B. rotunda, should be developed into a product nanoparticles. Objective: This work reports the synthesis of nanoparticles produced by ethanol extract of Boesenbergia rotunda rhizome loaded with chitosan and alginic acid, and its biological activity test as antioxidant. Method: The synthesis of nanoparticles used an ionic gelation. Activity of the nanoparticle products as antioxidant was tested by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Results: This work showed that nanoparticles chitosan produced by ethanol extract B. rotunda can be synthesized at a concentration (% w/v) of chitosan/ Na-TPP (sodium tripolyphosphate) and ratio of 8: 1, the size range of the nanoparticles were 389 to 877 nm, with a zeta potential of + 41.87 mV, and precentage nanoparticle 98.1%. The corresponding nanoparticles alginic acid can be synthesized at a concentration (% w/v) of alginic acid/ Ca2+ and ratio of 5: 1, the size range of the nanoparticles were 197 to 877 nm, with a zeta potential of -82.1 mV, and precentage nanoparticle 90.2%. Furthermore, nanoparticles result of the combination of alginic acid –chitosan can be synthesized at a concentration (% w/v) alginic acid/chitosan/ca2+ and ratio of 6.7: 2: 1, the size range of the nanoparticle were 226 to 877 nm, and precentage nanoparticle 29.7%. The morphology of each nanoparticle products was spherical and a smooth surface. The chitosan-alginic acid nanoparticles show higher activity than the starting material ethanol extract of B. rotunda.

Synergistic combination of fluoro chalcone and doxorubicin on HeLa cervical cancer cells by inducing apoptosis

Doxorubicin (Dox), a primary chemotherapeutic agent used for cancer treatment is known to have various side effect included multidrug resistance (MDR) phenomenon. Combination chemotherapy is one of some approaches to reduce Dox side effect. Chalcones have been reported to reduce the proliferation of many cancer cells. The research were conducted to investigate the cytotoxic activity and apoptosis induction of a chalcone derivate which is containing fluoro substituent [1 - (4” - fluorophenyl) -3 - (4’ - hydroxy - 3’ - methoxyphenyl) - 2 - propene - 1 -on] (FHM) and its combination with Dox on HeLa cells line. The observation of the cytotoxic activity was conducted using MTT [3 - (4, 5 - dimethyl thiazol - 2 - y1) - 2.5 - diphenyltetrazolium bromide] assay. Apoptosis induction was determined by flow cytometric. The changes of cell morphology were observed using phase contrast microscopy. The combination index (CI) was used to determine the effect of the combination. The study showed that FHM inhibited the H...

Activity of Oligoresveratrols from Stem Bark of Hopea mengarawan (Dipterocarpaceae) as Hydroxyl Radical Scavenger

The term of allelopathy refers to chemical interactions (inhibitory or stimulatory) between plants, between plants and microorganisms, and between microorganisms. The wealth of information on the processes, procedures, and practices of allelopathy has contributed to understanding this field of science. Recently, researches of allelopathy have been conducted in laboratory, greenhouse, and field with multifaceted standpoint in some concerning area: (i) allelochemicals identifications and screening test; (ii) ecological and physiological aspects of allelopathy; (iii) genetic studies and the possibilities of using plant breeding or genetic manipulation to enhance allelopathic varieties; (iv) the use of allelopathic potential in the biological control, including as natural pesticide, of weeds and plant diseases as eco-friendly approach for sustainable agriculture scheme. Key words: Allelochemicals, biological control, sustainable agriculture

Aktivitas Oligoresveratrol dari Kulit Batang Hopea mengarawan (Dipterocarpaceae) sebagai Penangkap Radikal Hidroksil

Four oligoresveratrols ranging from dimer to tetramer, isolated from stem bark of Hopea mengarawan (Dipterocarpaceae) plants were tested for their activity as hydroxyl radical scavenger. The activity of these compounds was evaluated against the 2-deoxyribose degradation induced by the hydroxyl radical generated via a Fenton-type reaction. Result showed that balanocarpol, heimiol A, vaticanol G, and vaticanol B had IC50 3.83; 15.44; 2.01; and 4.71 μM, respectively. These results suggest that oligoresveratrols from stem bark of H. mengarawan maybe useful as potential sources of natural anti-oxidants.