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Featured researches published by Ssn Fernando.


Journal of Wound Care | 2015

Evaluation of bactericidal effect of three antiseptics on bacteria isolated from wounds

D.U.A Kumara; Ssn Fernando; J Kottahachchi; Dmbt Dissanayake; Giddad Athukorala; Ns Chandrasiri; K.W.N. Damayanthi; M.H.S.L. Hemarathne; A.A. Pathirana

OBJECTIVE Antiseptics are widely used in wound management to prevent or treat wound infections due to their proven wound healing properties regardless of their cytotoxicity. The objective of this study was to determine the bactericidal effects of three antiseptics on pathogens known to cause wound infections. METHOD The study was carried out at a tertiary care hospital and a university microbiology laboratory in Sri Lanka in 2013. The three acids (acetic acid, ascorbic acid and boric acid) in increasing concentration (0.5%, 0.75% and 1%) were tested against bacterial suspensions equivalent to 0.5 McFarland standard. The Bacteria isolates used were isolated from wound and standard strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. RESULTS There were 33 (68.8%) Coliforms, 10 (20.8%) Pseudomonas species, and 5 (10.4%) strains of Staphylococcus aureus. Acetic acid at concentration of 0.5% inhibited growth of 37 (77%) and 42 (87.5%) of tested isolates when exposed for 30 and 60 minutes, respectively. However 100% inhibition was achieved at four hours. At a concentration of 0.75%, 40 (83.3%) and 44 (91.7%) were inhibited when exposed for 30 and 60 minutes, respectively, with 100% inhibition at 4 hours. At concentration of 1%, 46 (95.8%) inhibition was seen at 30 minutes and 100% inhibition at 60 minutes. Ascorbic acid, at 0.5% and 0.75 % concentrations, inhibited growth of 45(93.7%) and 47(97.9%) of isolates respectively when exposed for 30 minutes. At these two concentrations, 100% inhibition was achieved when exposed for one hour. At 1% concentration, 100% inhibition was achieved at 30 minutes. Boric acid did not show bactericidal effect at concentrations of 0.5%, 0.75 % and 1%. Pseudomonas species were inhibited at 30 minutes by 0.5% acetic acid. Bactericidal effect against all the standard strains was seen with three acids at each concentration tested from 30 minutes onwards CONCLUSION Ascorbic acid was bactericidal for all organisms tested within the shortest exposure time at the lowest concentration compared to other two acids. Despite promising bactericidal effects, further studies warrant, as ongoing debates on toxicity of acids on tissue epithelialisation. Application of antiseptics for a shorter duration could overcome this problem without losing bactericidal activity. DECLARATION OF INTEREST The authors have no conflict of interest and no funding was received for this study.


Indian Journal of Endocrinology and Metabolism | 2014

Proportion of lower limb fungal foot infections in patients with type 2 diabetes at a tertiary care hospital in Sri Lanka

Tm Wijesuriya; Manjula Weerasekera; J Kottahachchi; K.N.P. Ranasinghe; M. S. S. Dissanayake; Shamini Prathapan; Tdcp Gunasekara; A Nagahawatte; Ld Guruge; Uditha Bulugahapitiya; Ssn Fernando

Background: Superficial fungal foot infection (SFFI) in diabetic patients increases the risk of developing diabetic foot syndrome. Sixteen percent of urban population is suffering from diabetes in Sri Lanka. As the diabetes patients are more prone to get fungal foot infections, early intervention is advisable owing to the progressive nature of the infection. There is no data on the prevalence of SFFIs in diabetic patients in Sri Lanka. Objective: To determine the etiological agents causing SFFI in patients with type 2 diabetes. Materials and Methods: Three hundred eighty five diabetic patients were included. Nail clippings and swabs were collected from the infected sites using the standard protocol. Laboratory identification was done and pathogens were identified to the species level by morpho physiological methods. Results: Clinically 295 patients showed SFFI, of which 255 (86%) were mycologically confirmed for infection. Out of 236 direct microscopy (KOH) positives, 227 (96%) were culture positive. Two hundred and fifty one patients (98%) with SFFI had diabetes for more than 10 years. Of the patients with SFFIs 92% had >100 mg/dl FBS and 81% had >140 mg/dl PPBS levels and 80% had both elevated FBS and PPBS. Non-dermatophyte fungal species were the commonest pathogens followed by yeast and dermatophytes. Conclusion: Aspergillus niger was the commonest pathogen followed by Candida albicans. SFFIs were seen significantly with the increasing age, gender, duration of diabetes and with less controlled glycaemic level.


Endoscopy International Open | 2013

Residual bioburden in reprocessed side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP)

D.L.N.L. Ubhayawardana; J Kottahachchi; Manjula Weerasekera; I.W.M.P. Wanigasooriya; Ssn Fernando; L M de Silva

Background and study aim: Worldwide some endoscopy units routinely continue to use manual reprocessing techniques for disinfection of side-view endoscopes. The aim of this study was to evaluate the outcome quality of manual reprocessing techniques for removal and inactivation of the bioburden from side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP) in a tertiary referral endotherapy unit in Sri Lanka. Methods: 102 samples obtained from two different flexible side-view endoscopes (Olympus TJF Q 180V and Olympus TJF 160 R) were tested for microbial growth. Three samples were collected each time; one swab from the tip before and another after manual reprocessing. The third sample was collected by flushing the working channel with sterile normal saline after manual reprocessing. Microorganisms were identified by culturing the samples. Result:: After reprocessing, culture-positive rates were 20 % and 9 % for the samples obtained from the tip and the working channel of the side-view endoscopes, respectively. Klebsiella spp. and Candida spp. were found to be the commonest microorganisms in the samples from the tips and from the working channels, respectively, of the reprocessed side-view endoscopes. Conclusion: There is a high culture-positive rate after reprocessing of the side-view endoscopes using the manual reprocessing procedure, despite strict adherence to the protocol for reprocessing.


Sri Lankan Journal of Infectious Diseases | 2013

Knowledge, attitudes and practices of hand hygiene among final year medical and nursing students at the University of Sri Jayewardenepura

Mhjd Ariyarathne; Tdcp Gunasekara; Mm Weerasekara; J Kottahachchi; Bp Kudavidanage; Ssn Fernando


Sri Lankan Journal of Anaesthesiology | 2009

BACTERIAL CONTAMINATION OF ANAESTHETISTS HANDS, PERSONAL MOBILE PHONES AND WRIST WATCHES USED DURING THEATRE SESSIONS

Tdcp Gunasekara; Bp Kudavidanage; Mk Peelawattage; F Meedin; Ld Guruge; G Nanayakkara; M Nanayakkara; Ssn Fernando


Galle Medical Journal | 2012

Knowledge, attitudes and practices regarding dengue fever in a suburban community in Sri Lanka

Tdcp Gunasekara; Vgns Velathanthiri; Mm Weerasekara; Ssn Fernando; Mk Peelawattage; D Guruge; S Fernando


Sri Lankan Journal of Infectious Diseases | 2012

The distribution and characteristics of Extended-Spectrum β-Lactamase producing Escherichia coli and Klebsiella species among urinary isolates in a tertiary care hospital

Dmbt Dissanayake; Ssn Fernando; Ns Chandrasiri


Galle Medical Journal | 2009

Aerobic bacteria associated with symptomatic gallstone disease and their antimicrobial susceptibility

Prl Gomes; Ssn Fernando; Dd Weerasekara; Vgns Velathanthiri; Msm Rizny; Mm Weerasekara; Rohan Mahendra


Sri Lankan Journal of Infectious Diseases | 2013

In vitro study to determine antimicrobial activity of selected Ayurvedic preparations against bacteria and fungi causing superficial skin infections

Plr Gomes; S. Hewageegana; J Kottahachchi; Giddad Athukorala; Manjula Weerasekera; Tdcp Gunasekera; Dmbt Dissanayake; Dfd Meedin; Ssn Fernando


Chemistry Central Journal | 2017

Enhanced antibacterial activity of TiO2 nanoparticle surface modified with Garcinia zeylanica extract

U. L. N. H. Senarathna; Ssn Fernando; Tdcp Gunasekara; Manjula Weerasekera; H. G. S. P. Hewageegana; N. D. H. Arachchi; H. D. Siriwardena; P. M. Jayaweera

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Manjula Weerasekera

University of Sri Jayewardenepura

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Tdcp Gunasekara

University of Sri Jayewardenepura

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J Kottahachchi

University of Sri Jayewardenepura

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T.D.C.P. Gunasekera

University of Sri Jayewardenepura

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Mm Weerasekara

University of Sri Jayewardenepura

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Dmbt Dissanayake

University of Sri Jayewardenepura

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Giddad Athukorala

University of Sri Jayewardenepura

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Mk Peelawattage

University of Sri Jayewardenepura

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D.L.N.L. Ubhayawardana

University of Sri Jayewardenepura

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Dd Weerasekara

University of Sri Jayewardenepura

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