Stanimir Yotov

Ultrasonic evaluation of uterine involution in Bulgarian Murrah buffalo after administration of oxytocin.

The aim of this study was to determine the time taken for complete uterine involution in Bulgarian Murrah buffaloes following normal parturition and oxytocin stimulated milking; and to establish the time course of the change in size of the uterine horns, the cervix and caruncles between parturition and involution by means of ultrasonography. There were 17 animals in the study aged 3-6 years and average parity of 2.17 ± 0.18. They were administered 20 IU oxytocin 15 min before each milking. Rectal palpation and transrectal ultrasonography were performed at 3 d intervals from Days 1 to 34 post partum. The involution of the non-gravid and gravid uterine horns, and the cervix was complete by Days 22 and 25 post partum when their diameters were 2.7 ± 0.4 cm, 2.8 ± 0.3 cm and 3.12 ± 0.4 cm, respectively. Caruncles underwent rapid regression until Day 10 post partum. It was not possible to determine the dimensions of the caruncles after that time. The cumulative percentage of animals whose uterus was located in the pelvic cavity increased from 24% at Day 10 post partum to 100% at Day 34 post partum. The combination of rectal palpation and transrectal ultrasonography provided a reliable method of evaluating changes in the uterus over time and determining the time of uterine involution. The present study showed that complete uterine involution, with the uterus located in the pelvic cavity, was achieved by Day 34 after parturition in all 17 Bulgarian Murrah buffaloes treated with oxytocin before milking.

Therapy of ovarian inactivity in postpartum Bulgarian Murrah buffaloes by PRID and Ovsynch estrus synchronization protocols

Objective The aim of the present study was to assess therapeutical effect of modified Ovsynch and PRID estrus synchronization protocols in Bulgarian Murrah buffalo with inactive ovaries during the low-breeding season.

Investigation on some biochemical parameters and effect of hormonal treatment in anoestrous dairy cows with cystic ovarian follicle

Abstract Objective To investigate some blood biochemical parameters in anoestrous dairy cows with cystic ovarian follicles (COF) during the first two months post partum and the effect of hCG-PGF2 α -GnRH treatment on their reproductive response. Methods The investigation was performed on 20 lactating Holstein cows, divided into two groups: control group ( n =8) and treated group ( n =12). All animals had cystic ovarian follicles, detected during the two consecutive transrectal ultrasound examinations at 7-day interval. Blood samples from each animal were collected on the day of second ultrasonography (Day 0) and subjected to a hormonal and biochemical analysis. Treated cows were subjected to another ultrasound examination on day of prostaglandin injection and the presence of corpus luteum was recorded. The control animals were not treated, while the treated group received 1 500 IU hCG on day 0, PGF2 α 500 mg on day 7 and 100 μg GnRH 48 hours latter. Eighteen hours after GnRH administration animals bearing corpus luteum on Day 7 were artificiallly inseminated. The last ultrasound examination was made forty days after start of the threapy. On the base of the obtained results, cumulative oestrous activity, cumulative ovulation rate, pregnancy rate and COF persistence were determined. Results The blood analysis in the both groups on Day 0 showed significantly ( P P P Conclusions The present study confirmed the thesis for low blood levels of glucose and inorganic phosphate in cows presenting cystic ovarian follicles. It also indicated significant improvement of cumulative oestrous activity and ovulation rate in animals subjected to hCG-PGF2 α -GnRH administration than non-treated animals. The used hormonal protocol could decrease cystic ovarian follicle persistence in dairy cows.

Induction of ovarian activity in B ulgarian M urrah buffaloes by hormonal treatment in the early postpartum period

Objective To determine the possibilities for induction of ovarian activity in Bulgarian Murrah buffaloes by hormonal treatment in the early postpartum period.