Stanisław Chwil

Micromorphology and ultrastructure of the floral nectaries of Polemonium caeruleum L. (Polemoniaceae)

The Polemoniaceae family forms flowers diverse in the terms of pollination methods and nectar types. The micromorphology of the nectary surface and the tissue structures as well as the ultrastructure of the cells of the floral nectaries in Polemonium caeruleum L. were examined using light, scanning and transmission electron microscopy. A bowl-shaped nectary, detached from the ovary, grows at its base. Its contour shows folds with depressions in the places where the stamens grow, forming five-lobed disc (synapomorphic character). Nectar is secreted through modified anomocytic stomata, which are formed in the epidermis covering the tip and the lateral wall of the projection located between the staminal filaments. The undulate nectary consists of a single-layered epidermis and three to nine layers of parenchymal cells. The cells of the nectary contain a dense cytoplasm, numerous plastids with an osmophilic stroma and starch grains, well-developed endoplasmic reticulum, as well as a large number of mitochondria interacting with the Golgi bodies. The ultrastructure of nectary cells indicates the granulocrine secretion mechanism and diversified transport of nectar.

Macronutrient Balance of Nickel-Stressed Lettuce Plants Grown under Different Sulfur Levels

ABSTRACT This study investigated whether intensive nutrition with sulfur–sulfate (S-SO4) (2, 6, or 9 mM S) of nickel-stressed (0, 0.0004, 0.04, or 0.08 mM Ni) butterhead lettuce cv. Justyna may improve macronutrient balance and reduce Ni bioaccumulation. Nickel exposure resulted in various unfavorable changes in the macronutrient content together with increase of Ni accumulation in the biomass. Intensive S nutrition of Ni-treated lettuce seems to have no beneficial effect on macronutrient balance. In general, it significantly reduced the root and foliar phosphorus (P), potassium (K), calcium (Ca), and S content and simultaneously did not affect the magnesium (Mg) content in the biomass. At the same time, the nitrogen (N) content was reduced in roots and elevated in shoots. Supplementation of Ni-exposed lettuce with high S doses raised in roots and reduced foliar Ni accumulation; however, Ni content in useable parts exceeded the acceptable limits established for consumption.

Effects of Protein Hydrolysate on Soil Fertility and Heavy-Metal Accumulation in Sinapis alba L.

ABSTRACT The aim of this study was to evaluate the usefulness of protein hydrolysate (Hemozym) for fertilization purposes used in unpolluted and cadmium-polluted soil. Three levels of Hemozym (0, 0.1, and 0.2 cm3 kg−1 dry weight [DW] soil) and cadmium (Cd) rate 5 mg kg−1 DW soil were used. The fertilizer value of Hemozym was evaluated based on characteristics of the sorptive complex and soil respiratory activity as well as based on the soil and plant heavy-metal content. Increased soil fertility due to the beneficial effect of Hemozym on the improvement in the physicochemical properties of the soil and its microbial activity. However, they do not allow us to determine unambiguously the effect of this fertilizer on solubility vs. binding of heavy metals that occur naturally in the soil (zinc [Zn], copper [Cu], and lead [Pb]) and of intentionally introduced Cd, in spite of an indicative tendency towards the conversion of metals into poorly soluble forms.