Stanley J. Kays

Mutation of a Chitinase-Like Gene Causes Ectopic Deposition of Lignin, Aberrant Cell Shapes, and Overproduction of Ethylene

Chitinase-like proteins have long been proposed to play roles in normal plant growth and development, but no mutations in chitinase-like genes have been obtained previously to support this hypothesis. In this study, we have shown that the gene responsible for the elp1 mutation in Arabidopsis encodes a chitinase-like protein (AtCTL1). Mutation of this chitinase-like gene caused ectopic deposition of lignin and aberrant shapes of cells with incomplete cell walls in the pith of inflorescence stems. The AtCTL1 gene was expressed in all organs during normal plant growth and development, but it was not induced by wounding, salicylic acid, pectin fragments, or ethylene. Consistent with its ubiquitous expression pattern, mutation of the AtCTL1 gene affected many aspects of plant growth and development, including exaggerated hook curvature, reduced length and increased diameter of hypocotyls in dark-grown seedlings, and reduced root length and increased number of root hairs in light-grown seedlings. The mutant phenotypes could be rescued partially by ethylene inhibitors, and ethylene production in the mutant was significantly greater than in the wild type. Together, these results suggest that AtCTL1, a chitinase-like gene, is essential for normal plant growth and development in Arabidopsis.

Preharvest factors affecting appearance

Abstract The appearance of fresh fruits and vegetables is a primary criterion in making purchasing decisions. Product appearance is characterized by size, shape, form, color, condition and absence of defects. A wide range of preharvest factors can modulate the appearance of the harvested product. These include: (1) biological factors (pathological, entomological, animal); (2) physiological factors (physiological disorders, nutritional imbalances, maturity); (3) environmental/cultural factors (e.g. climate, weather, soils, water relations, light intensity); (4) mechanical damage; (5) extraneous matter (growing medium, vegetable matter, chemical residues); and (6) genetic variation and aberrations. Creating and/or maintaining production conditions that minimize undesirable product appearance is essential. While field grading during harvest is utilized to eliminate a significant portion of product with substandard appearance, minimizing the occurrence of inferior product can significantly increase net profit.

Comparison of Odor-Active Compounds from Six Distinctly Different Rice Flavor Types

Using a dynamic headspace system with Tenax trap, GC-MS, GC-olfactometry (GC-O), and multivariate analysis, the aroma chemistry of six distinctly different rice flavor types (basmati, jasmine, two Korean japonica cultivars, black rice, and a nonaromatic rice) was analyzed. A total of 36 odorants from cooked samples were characterized by trained assessors. Twenty-five odorants had an intermediate or greater intensity (odor intensity >or= 3) and were considered to be major odor-active compounds. Their odor thresholds in air were determined using GC-O. 2-Acetyl-1-pyrroline (2-AP) had the lowest odor threshold (0.02 ng/L) followed by 11 aldehydes (ranging from 0.09 to 3.1 ng/L), guaiacol (1.5 ng/L), and 1-octen-3-ol (2.7 ng/L). On the basis of odor thresholds and odor activity values (OAVs), the importance of each major odor-active compound was assessed. OAVs for 2-AP, hexanal, ( E)-2-nonenal, octanal, heptanal, and nonanal comprised >97% of the relative proportion of OAVs from each rice flavor type, even though the relative proportion varied among samples. Thirteen odor-active compounds [2-AP, hexanal, ( E)-2-nonenal, octanal, heptanal, nonanal, 1-octen-3-ol, ( E)-2-octenal, ( E, E)-2,4-nonadienal, 2-heptanone, ( E, E)-2,4-decadienal, decanal, and guaiacol] among the six flavor types were the primary compounds explaining the differences in aroma. Multivariate analysis demonstrated that the individual rice flavor types could be separated and characterized using these compounds, which may be of potential use in rice-breeding programs focusing on flavor.

Dielectric spectroscopy of watermelons for quality sensing

Dielectric properties of four small-sized watermelon cultivars, grown and harvested to provide a range of maturities, were measured with an open-ended coaxial-line probe and an impedance analyser over the frequency range from 10 MHz to 1.8 GHz. Probe measurements were made on the external surface of the melons and also on tissue samples from the edible internal tissue. Moisture content and soluble solids content (SSC) were measured for internal tissue samples, and SSC (sweetness) was used as the quality factor for correlation with the dielectric properties. Individual dielectric constant and loss factor correlations with SSC were low, but a high correlation was obtained between the SSC and permittivity from a complex-plane plot of dielectric constant and loss factor, each divided by SSC. However, SSC prediction from the dielectric properties by this relationship was not as high as expected (coefficient of determination about 0.4). Permittivity data (dielectric constant and loss factor) for the melons are presented graphically to show their relationships with frequency for the four melon cultivars and for external surface and internal tissue measurements. A dielectric relaxation for the external surface measurements, which may be attributable to a combination of bound water, Maxwell–Wagner, molecular cluster or ion-related effects, is also illustrated. Coefficients of determination for complex-plane plots, moisture content and SSC relationship, and penetration depth are also shown graphically. Further studies are needed for determining the practicality of sensing melon quality from their dielectric properties.

Chinese chestnut production in the United States: practice, problems, and possible solutions.

There are fewer than 162 ha of commercial Chinese chestnut orchards in the United States, with approximately half of these in the Southeast. Large numbers of Chinese chestnut seedlings are planted annually in the United States for home and game food production; however, knowledge about chestnut propagation, fertilization, pests, harvest, storage and marketing is not adequate. There is little commercial marketing of U.S.-grown Chinese chestnuts through roadside markets and local farmers’ markets as the supply is low and unpredictable. However, we yearly import 4.5 mill kg of European chestnuts to satisfy the U.S. market. Successful chestnut orchards will require vegetatively propagated selections, intensive management, mechanical harvesting, and control of insect and disease pests.

Common names of commercially cultivated vegetables of the world in 15 languages

The cultivated vegetable crops of the world are listed by family and Latin binomial, followed by the common name(s) in English, Arabic, Chinese (Mandarin), Danish, Dutch, French, German, Hindi, Italian, Japanese, Malay, Portuguese, Russian, Spanish, and Tagalog. The edible portion of the plant utilized for food and the general method of preparation were reviewed and listed.RésuméApresentase uma lista das culturas olerícolas cultivadas no Mundo organizadas por famílias e os seus nomes latinos seguidos dos respectivos nomes vulgares em Inglês, Árabe, Chinês (Mandarin), Dinamarquês, Holandês, Francês, Alemão, Hindu, Italiano, Japonês, Malaio, Português, Russo, Espanhol e Tagalogue. Para cada uma das culturas refere-se qual a sua parte comestível e o seu modo de preparação na alimentação.

Jerusalem artichoke growth, development, and field storage. II. Carbon and nutrient element allocation and redistribution

Abstract Jerusalem artichokes store carbon (C) predominately in the form of inulin, a functional food of increasing interest due to its dietary health benefits for humans and calorie replacement potential in processed foods. To better understand the developmental and agronomic requirements of this crop, the allocation of C and nutrient elements into individual plant parts (stems, leaves, stolons, tubers, ‘seed’ tuber, and roots) was monitored at 2‐week intervals throughout the entire growth cycle of the cultivar ‘Sunchoke’ grown on a Cecil sandy clay loam. Accumulation patterns of C and nutrient elements in individual plant parts were generally in accordance to the pattern of dry matter accumulation, though concentration patterns differed. Overall, nutrient element levels in vegetative structures decreased with the onset of rapid tuber development. Leaves had the highest nutrient levels, except iron (Fe) and sodium (Na). The concentrations of nitrogen (N), phosphorus (P), potassium (K), sulfur (S), and co...

Jerusalem artichoke growth, development, and field storage. I. Numerical assessment of plant part development and dry matter acquisition and allocation

Abstract Expanding commercial interest in the use of inulin as a bulking agent for artificial sweeteners, dietary fiber health supplement, fat replacement for processed foods, feed stock for fructose syrups, and a wide range of potential industrial products, has stimulated research on inulin‐containing crops such as the Jerusalem artichoke. To better understand the developmental physiology of the crop and to identify potential breeding objectives, the temporal pattern of development of individual plant parts (shoots, branches, leaves, flowers, stolons, tubers, and roots) and the allocation of dry matter into the same plant parts were monitored in the cultivar ‘Sunchoke’ over the entire growing season and during in situ field storage during the early winter, 32 weeks after planting. While number of shoots (∼9) peaked in week 10, the number of branches (42.8), stolons (49.4), and tubers (85.5) reached a maximum 24–28 weeks after planting. Number of leaves (∼525) peaked between weeks 20–24 after planting, as...

Relating sensory descriptors to volatile components in flavor of specialty rice types.

Flavor is a key factor contributing to consumer acceptance and repeat purchase of rice. Plant breeders focus on production yield and ignoring quality traits because there are no readily useable tools to evaluate quality. A systematic approach is needed for rice breeders to select rice with favorable flavor traits. Descriptive sensory analysis combined with chemical analysis provided an insight of sensory significance to interpret chemical data for a better understanding approach of rice flavor. This study was aimed to develop prediction models for sensory descriptors based on the volatile components derived from the gas chromatography-olfactometry (GC-O) that would be useful to help select rice cultivars containing a satisfactory flavor to produce improved quality in rice breeding programs. Thirteen Korean specialty rice samples were evaluated for their flavor components using descriptive analysis and GC-O. Nineteen aroma attributes in cooked specialty rice samples were evaluated by 8 trained panelists and statistically correlated to the concentration of aroma-active compounds derived from GC-O analysis. Prediction models were developed for most aroma descriptors including popcorn, cooked grain, starchy, woody, smoky, grain, corn, hay-like, barny, rancid, waxy, earthy, and sweet aroma using stepwise multiple linear regression. (E,E)-2, 4-decadienal, naphthalene, guaiacol, (E)-2-hexenal, 2-acetyl-1-pyrroline, 2-heptanone contributed most to these sensory attributes. These models help provide a quantitative link between sensory characteristics of commercial rice samples and aroma volatile components desirable in developing a rapid analytical method for use by rice breeders to screen progeny for superior flavor quality.

Attraction of adult sweet potato weevils, Cylas formicarius elegantulus (Summers), (Coleoptera: Curculionidae), to sweet potato leaf and root volatiles.

A dual-choice olfactometer was developed to study the responses of sweet potato weevils,Cylas formicarius elegantulus (Summers), to volatiles from the sweet potato,Ipomoea batatas (L.) Lam. Both males and females were attracted by volatiles from sweet potato leaves and a methylene chloride leaf extract. Females, but not males, responded to volatiles from storage roots and a methylene chloride root extract. Leaves and storage roots from four sweet potato cultivars (Centennial, Jewel, Resisto, and Regal) were attractive to female weevils; however, the attractant response varied with cultivar. GC profiles from leaf and root extracts, and GC-MS analysis of leaf extract, for Jewel cultivar enabled the volatile peaks to be identified as sesquiterpenes.