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Dive into the research topics where Steffen Helmling is active.

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Featured researches published by Steffen Helmling.


Gut | 2006

Anti-ghrelin Spiegelmer NOX-B11 inhibits neurostimulatory and orexigenic effects of peripheral ghrelin in rats

Peter Kobelt; Steffen Helmling; Andreas Stengel; Britta Wlotzka; V. Andresen; Burghardt F Klapp; Bertram Wiedenmann; Sven Klussmann; Hubert Mönnikes

Background and aims: Ghrelin, the natural ligand of the growth hormone secretagogue receptor 1a, is the most powerful peripherally active orexigenic agent known. In rodents, ghrelin administration stimulates growth hormone release, food intake, and adiposity. Because of these effects, blocking of ghrelin has been widely discussed as a potential treatment for obesity. Spiegelmer NOX-B11 is a synthetic l-oligonucleotide, which was previously shown to bind ghrelin. We examined the effects of NOX-B11 on ghrelin induced neuronal activation and food intake in non-fasted rats. Methods: Animals received various doses of NOX-B11, inactive control Spiegelmer, or vehicle intravenously. Ghrelin or vehicle was administered intraperitoneally 12 hours later and food intake was measured over four hours. Neuronal activation was assessed as c-Fos-like immunoreactivity in the arcuate nucleus. Results: Treatment with NOX-B11 30 nmol suppressed ghrelin induced c-Fos-like immunoreactivity in the arcuate nucleus and blocked the ghrelin induced increase in food intake within the first half hour after ghrelin injection (mean 1.13 (SEM 0.59) g/kg body weight; 4.94 (0.63) g/kg body weight versus 0.58 (0.58) g/kg body weight; p<0.0001). Treatment with NOX-B11 1 nmol or control Spiegelmer had no effect whereas treatment with NOX-B11 10 nmol showed an intermediate effect on ghrelin induced food intake. Conclusions: Spiegelmer NOX-B11 suppresses ghrelin induced food intake and c-Fos induction in the arcuate nucleus in rats. The use of an anti-ghrelin Spiegelmer could be an innovative new approach to inhibit the biological action of circulating ghrelin. This may be of particular relevance to conditions associated with elevated plasma ghrelin, such as the Prader-Willi syndrome.


Molecular and Cellular Endocrinology | 2011

Ghrelin neutralization during fasting-refeeding cycle impairs the recuperation of body weight and alters hepatic energy metabolism

Susana Sangiao-Alvarellos; Steffen Helmling; María J. Vázquez; Sven Klussmann; Fernando Cordido

Ghrelin, a hormone whose levels increase during food deprivation, plays a pivotal role in the regulation of food intake, energy metabolism and storage, as well as in insulin sensitivity. Here, we investigated the effects of acyl-ghrelin neutralization with the acyl-ghrelin-binding compound NOX-B11(2) during the fasting-refeeding cycle. Our data demonstrate that ghrelin neutralization with NOX-B11(2) impairs recuperation of lost body weight after food deprivation. Analysis of enzymes involved in glucose and lipid metabolism in liver of fed, fasted and refed rats revealed that neutralization of acyl-ghrelin resulted in minor decreases in the enzymes of glycolytic and lipogenic pathways during fasting. However, during refeeding these enzymes as well as glycogen levels recovered more slowly when acyl-ghrelin was blocked. The high levels of ghrelin in response to food deprivation may contribute to an adequate decrease in hepatic glycolytic and lipogenic enzymes and aid in the recovery of body weight and energetic reserves once food becomes available after the fasting period.


Nucleosides, Nucleotides & Nucleic Acids | 2003

A new class of Spiegelmers containing 2'-fluoro-nucleotides

Steffen Helmling; Elisabeth Moyroud; W. Schroeder; I. Roehl; F. Kleinjung; Sandra Stark; Gregor Bahrenberg; Clemens Gillen; Sven Klussmann; Stefan Vonhoff

Abstract Synthesis of 2′-fluoro-nucleosides from L-arabinose in order to perform the synthesis of 2′-fluoro-Spiegelmers binding to a neuropeptide.


Proceedings of the National Academy of Sciences of the United States of America | 2004

Inhibition of ghrelin action in vitro and in vivo by an RNA-Spiegelmer

Steffen Helmling; Christian Maasch; Dirk Eulberg; Klaus Buchner; Werner Schröder; Christian Lange; Stefan Vonhoff; Britta Wlotzka; Matthias H. Tschöp; Stefan Rosewicz; Sven Klussmann


Endocrinology | 2006

Ghrelin Neutralization by a Ribonucleic Acid-SPM Ameliorates Obesity in Diet-Induced Obese Mice

Lauren P. Shearman; Sheng-Ping Wang; Steffen Helmling; D. Sloan Stribling; Paul Mazur; Lan Ge; Liyang Wang; Sven Klussmann; D. Euan MacIntyre; Andrew D. Howard; Alison M. Strack


Archive | 2006

Ghrelin binding nucleic acids

Steffen Helmling; Dirk Eulberg; Christian Maasch; Sven Klussmann


Archive | 2004

Nucleic acids specifically binding bioactive ghrelin

Sven Klussmann; Steffen Helmling; Dirk Eulberg; Christian Maasch; Klaus Buchner


Archive | 2003

CGRP binding nucleic acids

Axel Vater; Christian Maasch; Florian Jarosch; Mathias Bell; Steffen Helmling; Bernd Eschgfäller; Elisabeth Moyroud; Sandra Stark; Sven Klussmann; Thorsten Ruppert; Gregor Bahrenberg; Clemens Gillen; Klaus Schiene


Archive | 2006

ACIDOS NUCLEICOS QUE SE UNEN ESPECIFICAMENTE A GHRELIN BIOACTIVO

Sven Klussmann; Steffen Helmling; Dirk Eulberg; Christian Maasch; Klaus Buchner


Archive | 2006

Ghrelinebindende nucleic acids.

Florian Jarosch; Dirk Eulberg; Christian Maasch; Steffen Helmling; Sven Klussmann

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Christian Maasch

Max Delbrück Center for Molecular Medicine

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Klaus Buchner

Free University of Berlin

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