Stephan Herminghaus

Self-driven jamming in growing microbial populations.

In natural settings, microbes tend to grow in dense populations [1–4] where they need to push against their surroundings to accommodate space for new cells. The associated contact forces play a critical role in a variety of population-level processes, including biofilm formation [5–7], the colonization of porous media [8, 9], and the invasion of biological tissues [10–12]. Although mechanical forces have been characterized at the single cell level [13–16], it remains elusive how collective pushing forces result from the combination of single cell forces. Here, we reveal a collective mechanism of confinement, which we call self-driven jamming, that promotes the build-up of large mechanical pressures in microbial populations. Microfluidic experiments on budding yeast populations in space-limited environments show that self-driven jamming arises from the gradual formation and sudden collapse of force chains driven by microbial proliferation, extending the framework of driven granular matter [17–20]. The resulting contact pressures can become large enough to slow down cell growth, to delay the cell cycle in the G1 phase, and to strain or even destroy the microenvironment through crack propagation. Our results suggest that self-driven jamming and build-up of large mechanical pressures is a natural tendency of microbes growing in confined spaces, contributing to microbial pathogenesis and biofouling [21–26].

The role of local instabilities in fluid invasion into permeable media

Wettability is an important factor which controls the displacement of immiscible fluids in permeable media, with far reaching implications for storage of CO2 in deep saline aquifers, fuel cells, oil recovery, and for the remediation of oil contaminated soils. Considering the paradigmatic case of random piles of spherical beads, fluid front morphologies emerging during slow immiscible displacement are investigated in real time by X-ray micro–tomography and quantitatively compared with model predictions. Controlled by the wettability of the bead matrix two distinct displacement patterns are found. A compact front morphology emerges if the invading fluid wets the beads while a fingered morphology is found for non–wetting invading fluids, causing the residual amount of defending fluid to differ by one order of magnitude. The corresponding crossover between these two regimes in terms of the advancing contact angle is governed by an interplay of wettability and pore geometry and can be predicted on the basis of a purely quasi–static consideration of local instabilities that control the progression of the invading interface.

Vesicles-on-a-chip: A universal microfluidic platform for the assembly of liposomes and polymersomes

Abstract.In this study, we present a PDMS-based microfluidic platform for the fabrication of both liposomes and polymersomes. Based on a double-emulsion template formed in flow-focusing configuration, monodisperse liposomes and polymersomes are produced in a controlled manner after solvent extraction. Both types of vesicles can be formed from the exact same combination of fluids and are stable for at least three months under ambient storage conditions. By tuning the flow rates of the different fluid phases in the flow-focusing microfluidic design, the size of the liposomes and polymersomes can be varied over at least one order of magnitude. This method offers a versatile tool for future studies, e.g., involving the encapsulation of biological agents and the functionalization of artificial cell membranes, and might also be applicable for the controlled fabrication of hybrid vesicles.Graphical abstract

Liquid morphologies and capillary forces between three spherical beads.

Equilibrium shapes of coalesced pendular bridges in a static assembly of spherical beads are computed by numerical minimization of the interfacial energy. Our present study focuses on generic bead configurations involving three beads, one of which is in contact to the two others while there is a gap of variable size between the latter. In agreement with previous experimental studies, we find interfacial trimer morphologies consisting of three coalesced pendular bridges, and dimers of two coalesced bridges. In a certain range of the gap opening we observe a bistability between the dimer and trimer morphology during changes of the liquid volume. The magnitude of the corresponding capillary forces in presence of a trimer or dimer depends, besides the gap opening, only on the volume or Laplace pressure of the liquid. For a given Laplace pressure, and for the same gap opening, the capillary forces induced by a trimer are only slightly larger than the corresponding forces in the presence of three pendular bridges. This observation is consistent with a plateau of capillary cohesion in terms of the saturation of a wetting liquid in the funicular regime, as reported in the experimental work [Scheel et al., Nat. Mater. 7, 189 (2008)1476-112210.1038/nmat2117].

Dimensionality matters in the collective behaviour of active emulsions

Abstract.The behaviour of artificial microswimmers consisting of droplets of a mesogenic oil immersed in an aqueous surfactant solution depends qualitatively on the conditions of dimensional confinement; ranging from only transient aggregates in Hele-Shaw geometries to hexagonally packed, convection-driven clusters when sedimenting in an unconfined reservoir. We study the effects of varying the swimmer velocity, the height of the reservoir, and the buoyancy of the droplet swimmers. Two simple adjustments of the experimental setting lead to a suppression of clustering: either a decrease of the reservoir height below a certain value, or a match of the densities of droplets and surrounding phase, showing that the convection is the key mechanism for the clustering behaviour.Graphical abstract

Direct visualization of spatiotemporal structure of self-assembled colloidal particles in electrohydrodynamic flow of a nematic liquid crystal.

Characterization of spatiotemporal dynamics is of vital importance to soft matter systems far from equilibrium. Using a confocal laser scanning microscopy, we directly reveal three-dimensional motion of surface-modified particles in the electrohydrodynamic convection of a nematic liquid crystal. Particularly, visualizing a caterpillar-like motion of a self-assembled colloidal chain demonstrates the mechanism of the persistent transport enabled by the elastic, electric, and hydrodynamic contributions. We also precisely show how the particles trajectory is spatially modified by simply changing the surface boundary condition.

The minimization of mechanical work in vibrated granular matter

Experiments and computer simulations are carried out to investigate phase separation in a granular gas under vibration. The densities of the dilute and the dense phase are found to follow a lever rule and obey an equation of state. Here we show that the Maxwell equal-areas construction predicts the coexisting pressure and binodal densities remarkably well, even though the system is far from thermal equilibrium. This construction can be linked to the minimization of mechanical work associated with density fluctuations without invoking any concept related to equilibrium-like free energies.

Filling transitions on rough surfaces: Inadequacy of Gaussian surface models.

We present numerical studies of wetting on various topographic substrates, including random topographies. We find good agreement with recent predictions based on an analytical interface-displacement-type theory, except that we find critical end points within the physical parameter range. As predicted, Gaussian random surfaces are found to behave qualitatively different from non-Gaussian topographies. This shows that Gaussian random processes as models for rough surfaces must be used with great care, if at all, in the context of wetting phenomena.

Spontaneous chiral symmetry breaking in collective active motion.

Chiral symmetry breaking is ubiquitous in biological systems, from DNA to bacterial suspensions. A key unresolved problem is how chiral structures may spontaneously emerge from achiral interactions. We study a simple model of active swimmers in three dimensions that effectively incorporates hydrodynamic interactions. We perform large-scale molecular dynamics simulations (up to 10(6) particles) and find long-lived metastable collective states that exhibit chiral organization although the interactions are achiral. We elucidate under which conditions these chiral states will emerge and grow to large scales. To explore the complex phase space available to the system, we perform nonequilibrium quenches on a one-dimensional Lebwohl-Lasher model with periodic boundary conditions to study the likelihood of formation of chiral structures.

Radar for tracer particles

We introduce a radar system capable of tracking a 5 mm spherical target continuously in three dimensions. The 10 GHz (X-band) radar system has a transmission power of 1 W and operates in the near field of the horn antennae. By comparing the phase shift of the electromagnetic wave traveling through the free space with an IQ-mixer, we obtain the relative movement of the target with respect to the antennae. From the azimuth and inclination angles of the receiving antennae obtained in the calibration, we reconstruct the target trajectory in a three-dimensional Cartesian system. Finally, we test the tracking algorithm with target moving in circular as well as in pendulum motions and discuss the capability of the radar system.