Stéphane Descloux

Gross CO2 and CH4 emissions from the Nam Ngum and Nam Leuk sub-tropical reservoirs in Lao PDR.

Gross CO2 and CH4 emissions (degassing and diffusion from the reservoir) and the carbon balance were assessed in 2009-2010 in two Southeast Asian sub-tropical reservoirs: the Nam Ngum and Nam Leuk Reservoirs (Lao PDR). These two reservoirs are within the same climatic area but differ mainly in age, size, residence time and initial biomass stock. The Nam Leuk Reservoir was impounded in 1999 after partial vegetation clearance and burning. However, GHG emissions are still significant 10 years after impoundment. CH4 diffusive flux ranged from 0.8 (January 2010) to 11.9 mmol m(-2) d(-1) (April 2009) and CO2 diffusive flux ranged from -10.6 (October 2009) to 38.2 mmol m(-2) d(-1) (April 2009). These values are comparable to other tropical reservoirs. Moreover, degassing fluxes at the outlet of the powerhouse downstream of the turbines were very low. The tentative annual carbon balance calculation indicates that this reservoir was a carbon source with an annual carbon export (atmosphere+downstream river) of about 2.2±1.0 GgC yr(-1). The Nam Ngum Reservoir was impounded in 1971 without any significant biomass removal. Diffusive and degassing CO2 and CH4 fluxes were lower than for other tropical reservoirs. Particularly, CO2 diffusive fluxes were always negative with values ranging from -21.2 (April 2009) to -2.7 mmol m(-2) d(-1) (January 2010). CH4 diffusive flux ranged from 0.1 (October 2009) to 0.6 mmol m(-2) d(-1) (April 2009) and no degassing downstream of the turbines was measured. As a consequence of these low values, the reservoir was a carbon sink with an estimated annual uptake of - 53±35 GgC yr(-1).

Development of 55 novel polymorphic microsatellite loci for the critically endangered Zingel asper L. (Actinopterygii: Perciformes: Percidae) and cross-species amplification in five other percids

By combining biotin-enrichment protocol and next generation pyrosequencing, through 454 GS-FLX Titanium technology, 55 polymorphic microsatellites loci with perfect motif were isolated from the Rhone streber (Zingel asper), a critically endangered European fish species. Eight multiplex PCR kits were optimised in order to genotype a total of 58 polymorphic loci, including three previously published loci. The level of genetic diversity was assessed for 68 Z. asper, 30 Sander lucioperca, 33 Perca fluviatilis and four Gymnocephalus schraetzer individuals. Amplification success was also assessed on Romanichthys valsanicola and Zingel streber using single individuals. These markers will be useful to investigate the population structure of the highly fragmented Rhone streber. They represent a powerful tool for conservation issues and evolutionary approaches of this endemic species. Moreover, part of our markers demonstrated applicability to other percid species, allowing for potential applications to fisheries and aquaculture management.

Methane and nitrous oxide annual emissions from an old eutrophic temperate reservoir

Two greenhouse gases -methane (CH4) and nitrous oxide (N2O) - were monitored monthly during one year (2011) at the Eguzon Reservoir in France. The objective of the study was to quantify for the first time in a temperate area the total emissions of these gases through the main emission pathways (diffusion and bubbling from the reservoir, degassing and downstream diffusion). The reservoir was impounded in 1926 and had, in 2011, a eutrophic status promoting high organic matter degradation and nitrification-denitrification, all favouring CH4 and N2O production. CH4 and N2O emissions were dominated by diffusion from the reservoir surface (respectively 78.0% and 92.3%). Ebullition was only observed for CH4 and accounted for 14.0% of total CH4 emissions. Downstream degassing and diffusion represented 8.1% of the total CH4 emissions and 7.7% of the total N2O emissions.

Total iron concentrations in waters and fish tissues in the Nam Theun 2 Reservoir area (Lao PDR)

Data on total iron concentrations in waters and freshwater fish tissues in man-made reservoirs are scarce, especially in Southeast Asia. Changes in total iron concentrations in water and in fish tissues were studied after the impoundment of the Nam Theun 2 Reservoir (central Lao PDR). Water quality parameters were monitored at 11 stations (reservoir, upstream area and downstream areas) from 2008 to 2010. In 2009 and 2010, total iron concentrations were measured in three different fish tissues (viscera, gills and flesh) from 14 species belonging to the omnivorous and carnivorous feeding groups. The results indicated that iron concentrations in the water were influenced by the reservoir impoundment during the first year after the creation of the reservoir. Intra-annual variations of the total iron concentration in these waters appeared to be driven by the soil leaching processes mainly during the wet season. In fish, total iron accumulated preferentially in viscera, followed by gills and flesh. Iron concentration was highly species dependant and related to the ecology of the species whereas feeding habits (omnivorous or carnivorous) did not influence total iron concentration in fish tissues. Finally, reservoir impoundment did not affect iron concentrations in fish from the reservoir and from both downstream areas.

Advantages and disadvantages of an aerating weir in a tropical zone

The creation o f reservoirs results in the flooding of soils or forest. A small fraction ofthis flooded organic matter (soils, tree, peat, shrubs) will decompose and produce greenhouse gases (GHG) that will be emitted at the water-air interface. The 3 majors GHG are carbon dioxide (C02), methane (CH4) and nitrous oxide (N20). Reservoirs in inter-tropical zone are more likely to develop hypolimnion (bottom waters) with large oxygen deficits (anoxia). This happens rapidly, within the first year after flooding, and is primarily caused by the decomposition of a fraction o f submerged vegetation related, among other things, to a high water temperature (>25 oq combined with a higher bacterial activity. These conditions are rarely observed in boreal reservoirs (ThEMBLAY et al. 2005). Water in hydroelectric reservoirs is generally taken from the bottom layer to produce electricity with the turbines. This anoxic bottom water is also supersaturated in CH4 and C02 in comparison to the atrnosphere. To maintain an oxygen content compatible with aquatic life at all points in the downstream river, the solution consists in supplying oxygen and eliminating all or part of the dissolved gases, in particular CH4• Among the possible aerating devices found in the literature, weirs seemed to be the most efficient. Our objective was to demonstrate the efficiency o f an aerating weir.