Stéphanie Arnoult

Cell wall compositional modifications of Miscanthus ecotypes in response to cold acclimation.

Miscanthus, a potential energy crop grass, can be damaged by late frost when shoots emerge too early in the spring and during the first winter after planting. The effects of cold acclimation on cell wall composition were investigated in a frost-sensitive clone of Miscanthus x giganteus compared to frost-tolerant clone, Miscanthus sinensis August Feder, and an intermediate frost-tolerant clone, M. sinensis Goliath. Cellulose and lignin contents were higher in M. x giganteus than in the M. sinensis genotypes. In ambient temperature controls, each clone displayed different glucuronoarabinoxylan (GAX) contents and degree of arabinose substitution on the xylan backbone. During cold acclimation, an increase in (1→3),(1→4)-β-D-glucan content was observed in all genotypes. Uronic acid level increased in the frost sensitive genotype but decreased in the frost tolerant genotypes in response to cold. In all clones, major changes in cell wall composition were observed with modifications in phenylalanine ammonia-lyase (PAL) and cinnamyl alcohol dehydrogenase (CAD) activities in both non- and cold-acclimated experiments. A large increase in CAD activity under cold stress was displayed in each clone, but it was largest in the frost-tolerant clone, M. sinensis August Feder. The marked increase in PAL activity observed in the frost-tolerant clones under cold acclimation, suggests a reorientation of the products towards the phenylpropanoid pathway or aromatic synthesis. How changes in cell wall physical properties can impact frost tolerance is discussed.

A Review on Miscanthus Biomass Production and Composition for Bioenergy Use: Genotypic and Environmental Variability and Implications for Breeding

The lignocellulosic C4 perennial crop miscanthus and, more particularly, one of its species, Miscanthus × giganteus, are especially interesting for bioenergy production because they combine high biomass production with a low environmental impact. However, few varieties are available, which is risky due to disease susceptibility. Gathering worldwide references, this review shows a high genotypic and environmental variability for traits of interest related to miscanthus biomass production and composition, which may be useful in breeding programs for enhancing the availability of suitable clones for bioenergy production. The M. × giganteus species and certain clones in the Miscanthus sinensis species seem particularly interesting due to high biomass production per hectare. Although the industrial requirements for biomass composition have not been fully defined for the different bioenergy conversion processes, the M. × giganteus and Miscanthus sacchariflorus species, which show high lignin contents, appear more suitable for thermochemical conversion processes. In contrast, the M. sinensis species and certain M. × giganteus clones with low lignin contents were interesting for biochemical conversion processes. The M. sacchariflorus species is also interesting as a progenitor for breeding programs, due to its low ash content, which is suitable for the different bioenergy conversion processes. Moreover, mature miscanthus crops harvested in winter seem preferred by industry to enhance efficiency and reduce the expense of the processes. This investigation on miscanthus can be extrapolated to other monocotyledons and perennial crops, which may be proposed as feedstocks in addition to miscanthus.

Miscanthus Clones Display Large Variation in Floral Biology and Different Environmental Sensitivities Useful for Breeding

A wider range of Miscanthus varieties is required to develop Miscanthus clones that are suitable for bioenergy production. For this reason, breeding programs need to be initiated using knowledge regarding the genetic influence on floral biological traits. The objective of the present study was to characterize the genotypic variation in flowering and panicle architecture traits in Miscanthus by studying (i) the clone effect on these traits and (ii) the clone sensitivity to environmental conditions. The flowering traits characterized were date of panicle emergence, date of flowering onset, and interval between these two traits. The panicle architecture traits characterized were total panicle length, longest panicle raceme size, raceme number per panicle, floral density, and total flower number per panicle. Eight clones were studied in a greenhouse under four environmental conditions including two day lengths (an 8-h short day length and a natural day length) and two temperature treatments (warm and cool). Miscanthus clones showed large differences in flowering and panicle architecture traits. Moreover, day length appeared to be the most important environmental factor creating differential clone sensitivities for the panicle emergence and the onset of flowering in contrast to temperature factor for the total flower number per panicle. In addition, the behavior of the clone Sacc was in contrast with that of the other clones for most of the traits studied. This knowledge will be useful to optimize the synchronization of flowering between Miscanthus clones for more successful breeding programs.

Influence of the radial stem composition on the thermal behaviour of miscanthus and sorghum genotypes

The hypothesis made is that thermal resistance of sorghum and miscanthus stem pieces taken at well-defined positions of the stem is simply related to their biochemical composition. For miscanthus, two different genotypes and two internode levels were selected. For each region, the stem was divided into three radial layers. For sorghum, two different genotypes were selected and the stem was divided into the same three radial layers. The results show that the thermal analysis is only sensitive to very large variations of compositions. But aside of such large composition differences, it is impossible to correlate thermal effects to biochemical composition even on very small size, well-identified pieces of plant materials. The interplay between sugar-based components, lignin and minerals is totally blurring the thermal response. Extreme care must be exercised when willing to explain why a given plant material has a thermal behaviour different of another plant material.

Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition

HIGHLIGHTS Biomass production and cell wall composition are differentially impacted by harvesting year and genotypes, influencing then cellulose conversion in miniaturized assay. Using a high-throughput miniaturized and semi-automated method for performing the pretreatment and saccharification steps at laboratory scale allows for the assessment of these factors on the biomass potential for producing bioethanol before moving to the industrial scale. The large genetic diversity of the perennial grass miscanthus makes it suitable for producing cellulosic ethanol in biorefineries. The saccharification potential and year variability of five genotypes belonging to Miscanthus × giganteus and Miscanthus sinensis were explored using a miniaturized and semi-automated method, allowing the application of a hot water treatment followed by an enzymatic hydrolysis. The studied genotypes highlighted distinct cellulose conversion yields due to their distinct cell wall compositions. An inter-year comparison revealed significant variations in the biomass productivity and cell wall compositions. Compared to the recalcitrant genotypes, more digestible genotypes contained higher amounts of hemicellulosic carbohydrates and lower amounts of cellulose and lignin. In contrast to hemicellulosic carbohydrates, the relationships analysis between the biomass traits and cellulose conversion clearly showed the same negative effect of cellulose and lignin on cellulose digestion. The miniaturized and semi-automated method we developed was usable at the laboratory scale and was reliable for mimicking the saccharification at the pilot scale using a steam explosion pretreatment and enzymatic hydrolysis. Therefore, this miniaturized method will allow the reliable screening of many genotypes for saccharification potential. These findings provide valuable information and tools for breeders to create genotypes combining high yield, suitable biomass composition, and high saccharification yields.