Stephen Alexander

Immunogenic structure of the influenza virus hemagglutinin

We chemically synthesized 20 peptides corresponding to 75% of the HA1 molecule of the influenza virus. Antibodies to the majority (18) of these peptides were capable of reacting with the hemagglutinin molecule. These 18 peptides are not confined to the known antigenic determinants of the hemagglutinin molecule, but rather are scattered throughout its three-dimensional structure. In contrast, antibody raised to intact hemagglutinin did not react with any of the 20 peptides. Taken together these results suggest that the immunogenicity of an intact protein molecule is not the sum of the immunogenicity of its pieces.

Endoglycosidases from Flavobacterium meningosepticum application to biological problems

Publisher Summary This chapter describes the purification and properties of these useful enzymes as well as some of the biological problems to which they have been applied. The enzymes are useful in studying (1) the role of N-linked carbohydrate side chains in the stability of glycoproteins and protection from proteolytic attack, (2) the influence of N-linked glycans on immune surveillance of glycoproteins, which is particularly relevant to the immune response against enveloped viruses, (3) the role of oligosaccharide side chains in specific intercellular interactions, (4) the precursor-product relationships of glycoproteins and whether molecular polymorphisms are because of multiple gene products or differential glycosylation of a single polypeptide backbone, (5) the use of potential sites of glycosylation predicted from nucleotide sequencing, (6) the isolation and detailed structural characterization of N-linked oligosaccharides, and (7) the role of N-linked carbohydrates on the biological activities of glycoprotein enzymes and hormones.

Mutants of dictyostelium discoideum blocked in expression of all members of the developmentally regulated discoidin multigene family

Mutant strains of D. discoideum are described that can complete morphogenesis and cytodifferentiation but which express vastly reduced levels of the galactose-binding lectins discoidin I and II (less than 1% and 1%-2% respectively) compared to the wild-type control. Mutant cells proceeding through development lack lectin activity, lectin protein, and specific lectin mRNA. In contrast, the genes encoding these proteins are present in their wild-type configurations in the genome. Since these proteins are encoded by four to five discrete genes, the mutations in these strains are most likely in genes involved in the regulation of the expression of members of this multigene family. The results also indicate that the discoidin lectins may not be required for fruiting body construction in this organism. Finally, coupled with the recent ability to transform D. discoideum, these mutants open the way to identification and isolation of regulatory genes and their products.

Characterization of a timing mutant of Dictyostelium discoideum which exhibits “high frequency switching”

The preaggregative period of Dictyostelium discoideum is composed of two sequential rate-limiting components. The timing mutant FM-1 exhibits a decrease in the length of the preaggregative period and the interval between the maxifinger and early culminate II stage. In contrast, it is normal in all aspects of growth, in the sequence of morphogenetic stages, in spore formation, in the capacity to rapidly recapitulate morphogenesis, and in the erasure event and subsequent program of dedifferentiation. By the reciprocal shift experiment, it is demonstrated that FM-1 is completely missing the first of the two rate-limiting components comprising the preaggregative period. The FM-1 mutation is heritable and behaves as a single mutation mapping to linkage group II. However, the FM-1 variant switches at relatively high frequency to several other timing phenotypes with longer preaggregative periods which in turn switch at high frequency. The FM-1 phenotype is considered in terms of timing regulation, and the process of high frequency switching between timing phenotypes is compared to other newly discovered switching systems.