Stephen J. Pearson

Plyometric vs. isometric training influences on tendon properties and muscle output.

The purpose of this study was to concurrently determine the effect that plyometric and isometric training has on tendon stiffness (K) and muscle output characteristics to compare any subsequent changes. Thirteen men trained the lower limbs either plyometrically or isometrically 2–3 times a week for a 6-week period. Medial gastrocnemius tendon stiffness was measured in vivo using ultrasonography during ramped isometric contractions before and after training. Mechanical output variables were measured using a force plate during concentric and isometric efforts. Significant (p < 0.05) training-induced increases in tendon K were seen for the plyometric (29.4%; 49.0 < 10.8 to 63.4 < 9.2 N·mm-1) and isometric groups (61.6%; 43.9 < 2.5 to 71.0 < 7.4 N·mm-1). Statistically similar increases in rate of force development and jump height were also seen for both training groups, with increases of 18.9 and 58.6% for the plyometric group and 16.7 and 64.3% for the isometric group, respectively. Jump height was found to be significantly correlated with tendon stiffness, such that stiffness could explain 21% of the variance in jump height. Plyometric training has been shown to place large stresses on the body, which can lead to a potential for injury, whereas explosive isometric training has been shown here to provide similar benefits to that of plyometric training with respect to the measured variables, but with reduced impact forces, and would therefore provide a useful adjunct for athletic training programs within a 6-week time frame.

The omega-3 fatty acid, eicosapentaenoic acid (EPA), prevents the damaging effects of tumour necrosis factor (TNF)-alpha during murine skeletal muscle cell differentiation

BackgroundEicosapentaenoic acid (EPA) is a ώ-3 polyunsaturated fatty acid with anti-inflammatory and anti-cachetic properties that may have potential benefits with regards to skeletal muscle atrophy conditions where inflammation is present. It is also reported that pathologic levels of the pro-inflammatory cytokine tumour necrosis factor (TNF)-α are associated with muscle wasting, exerted through inhibition of myogenic differentiation and enhanced apoptosis. These findings led us to hypothesize that EPA may have a protective effect against skeletal muscle damage induced by the actions of TNF-α.ResultsThe deleterious effects of TNF-α on C2C12 myogenesis were completely inhibited by co-treatment with EPA. Thus, EPA prevented the TNF-mediated loss of MyHC expression and significantly increased myogenic fusion (p < 0.05) and myotube diameter (p < 0.05) indices back to control levels. EPA protective activity was associated with blocking cell death pathways as EPA completely attenuated TNF-mediated increases in caspase-8 activity (p < 0.05) and cellular necrosis (p < 0.05) back to their respective control levels. EPA alone significantly reduced spontaneous apoptosis and necrosis of differentiating myotubes (p < 0.001 and p < 0.05, respectively). A 2 hour pre-treatment with EPA, prior to treatment with TNF alone, gave similar results.ConclusionIn conclusion, EPA has a protective action against the damaging effects of TNF-α on C2C12 myogenesis. These findings support further investigations of EPA as a potential therapeutic agent during skeletal muscle regeneration following injury.

Effect of Acute Tensile Loading on Gender-Specific Tendon Structural and Mechanical Properties

Stretching is commonly used prior to exercise, as it is thought to reduce the risk of injury, and it is also used in the preconditioning of tendon grafts. As tendon properties have been shown to be different between genders, it is proposed that stretching will differentially affect the structure. Here we examine the effect of acute stretch on the mechanical properties of both male and female medial gastrocnemius tendon. Female [20 years ± 1 (SEM), n = 17] and male (22 years ± 1, n = 18) subjects underwent a 5‐min passive dorsiflexion stretch. Prior to and post stretch medial gastrocnemius tendon stiffness (K), length (l) and cross‐sectional area (csa) were measured using ultrasonography and dynamometry. Stiffness and Youngs modulus (ε) were significantly reduced with stretch for both genders (p < 0.05). Females showed significantly (p < 0.05) greater pre‐ to poststretch decreases in K (22.4 vs. 8.8%) and ε (20.5 vs. 8.4%) in comparison to males. The present results show that stretching acutely reduces stiffness of the medial gastrocnemius tendon in females and males, with females showing significantly greater change. The observed disparity between genders may be due in part to variations in tendon moment arm and intrinsic differences in tendon composition. These differential changes in tendon mechanical properties have functional, motor control, and injury risk implications, as well as possible implications for preconditioning of tendon grafts.

Influence of time of day on tendon compliance and estimations of voluntary activation levels.

We investigated the importance of tendon stiffness on estimations of central activation capacity (CAC). Patella tendon–force elongation relationship, quadriceps torque, and response to 50‐μs, 100‐HZ electrical twitch doublets were studied at ∼8:00 A.M. and ∼6:00 P.M. in 8 healthy young men. Results show that in the evening, as compared with the morning, tendon stiffness decreased by 20.2 ± 9.5% (P = 0.03) and time to twitch peak (Ttp) increased by 55% (P = 0.04). Tendon stiffness and Ttp correlated significantly (r = 0.28, P < 0.05). Whereas the CAC, calculated as the ratio of force preceding the interpolated twitch (IT) to the total force at the peak of the IT, showed no change with time of day, calculations that used twitch ratios suggested CAC decrements (−10.3%, P = 0.03, unpotentiated; −10%, P = 0.02, potentiated ratio) in the evening. This study shows that CAC estimations are invalid when tendon properties are not taken into account, an error that would have important implications in a clinical setting. Muscle Nerve, 2006

Acute changes in knee-extensors torque, fiber pennation, and tendon characteristics.

The aim of the current study was to examine the relationships between quadriceps torque, vastus lateralis pennation angle (θ), and patella tendon stiffness (K) at 07:45 and 17:45 h. Using short‐duration static contractions, simultaneous recordings were made of vastus lateralis (VL) electromyograph (EMG), θ and patella tendon K. Peak isometric extension torque (Peak torque Extcorr) increased by 29.4±6.5% at a knee angle of 70° (p=0.03) in the evening compared to the morning. In the contracted muscle, a 35.0±11.0% (p=0.02) time‐of‐day (TOD)‐related change in θ (to a greater evening compared to morning θ) was observed. Morning and evening measures of θ were also made, both at rest and at a standardized force level (250 N), to separate architecture change effects from increased torque capacity effects. Significant increments in θ in both the resting muscle (13.0±5.1%, p=0.046) and during the standardized exertions (8.0±3.1%, p=0.04) were observed in the evening versus the morning. Increases in θ with TOD were significantly correlated with the 40% (p=0.018) decrease in K both during the standardized contractions (r=0.788, p<0.001) and at rest (r=0.77, p=0.026). These data show that TOD affects K and θ and that these two important factors involved in in‐vivo muscle torque generation capacity are associated. The data also show that despite the potentially deleterious effects of the direction of the changes in both K and θ with TOD, peak torque Extcorr still shows a significant upward shift in the evening relative to the morning.

A comparison of maximal squat strength and 5-, 10-, and 20-meter sprint times, in athletes and recreationally trained men.

Abstract Comfort, P, Bullock, N, and Pearson, SJ. A comparison of maximal squat strength and 5-, 10-, and 20-meter sprint times, in athletes and recreationally trained men. J Strength Cond Res 26(4): 937–940, 2012—The purpose of this study was to identify whether there was a relationship between relative strength during a 1 repetition maximum (1RM) back squat and 5-, 10-, and 20-m sprint performances in both trained athletes and recreationally trained individuals. Professional rugby league players (n = 24) and recreationally trained individuals (n = 20) participated in this investigation. Twenty-meter sprint time and 1RM back squat strength, using free weights, were assessed on different days. There were no significant (p ≥ 0.05) differences between the well-trained and recreationally trained groups for 5-m sprint times. In contrast, the well-trained groups 10- and 20-m sprint times were significantly quicker (p = 0.004; p = 0.002) (1.78 + 0.06 seconds; 3.03 + 0.09 seconds) compared with the recreationally trained group (1.84 + 0.07 seconds; 3.13 + 0.11 seconds). The athletes were significantly stronger (170.63 + 21.43 kg) than the recreationally trained individuals (135.45 + 30.07 kg) (p = 0.01); however, there were no significant differences (p > 0.05) in relative strength between groups (1.78 + 0.27 kg/kg; 1.78 + 0.33 kg/kg, respectively). Significant negative correlations were found between 5-m sprint time and relative squat strength (r = −0.613, power = 0.96, p = 0.004) and between relative squat strength and 10- and 20-m sprint times in the recreationally trained group (r = −0.621, power = 0.51, p = 0.003; r = −0.604, power = 0.53, p = 0.005, respectively). These results, indicating that relative strength, are important for initial sprint acceleration in all athletes but more strongly related to sprint performance over greater distances in recreationally trained individuals.

Patellar tendon properties with fluctuating menstrual cycle hormones.

Burgess, KE, Pearson, SJ, and Onambélé, GL. Patellar tendon properties with fluctuating menstrual cycle hormones. J Strength Cond Res 24(8): 2088-2095, 2010-Debate continues over whether skeletal muscle performance and injury risk vary over the course of the menstrual cycle. Alterations in tendon properties may play a role in the potential fluctuations of both of these variables. The aim of the current study was to determine any association between menstrual cycle phase and corresponding levels of female sex hormones and tendon properties. Fifteen normally menstruating (28-32-day cycles) healthy females (age 23 ± 1 years, mass 63.1 ± 2.6 kg, height 1.66 ± 0.02 m) not taking any form of hormonal contraceptive took part in this study. In vivo patellar tendon properties and associated circulating hormonal levels were assessed on 3 occasions including days 3 ± 0.4, 13 ± 0.2, and 21 ± 0.3. Dynamometry, ultrasonography, electromyography, and biochemical assessment of circulating levels of estradiol and progesterone were utilized. No significant differences were seen in tendon mechanical properties among the 3 phases of the menstrual cycle (p > 0.05). Regressions were carried out and revealed that estrogen and maximal voluntary tendon force explained 17.8% (p = 0.043) of the variance in youngs modulus. Our findings link estrogen to a chronic, rather than an acute, impact on tendon behavior. These findings are relevant to clinical outcomes, exercise performance, and injury risk. In terms of tendon properties, menstrual cycle phase does not necessarily need to be considered when organizing training and competition schedules.

AN ELECTROMYOGRAPHICAL COMPARISON OF TRUNK MUSCLE ACTIVITY DURING ISOMETRIC TRUNK AND DYNAMIC STRENGTHENING EXERCISES

Comfort, P, Pearson, SJ, and Mather, D. An electromyographical comparison of trunk muscle activity during isometric trunk and dynamic strengthening exercises. J Strength Cond Res 25(X): 000-000, 2010-The purpose of this study was to compare rectus abdominis and erector spinae muscle activity during isometric (prone bridge [PB] and superman [SM]) and dynamic strengthening exercises (back squat, front squat [FS], and military press). Participants (n = 10, age 21.8 ± 2.6 years; body mass 82.65 ± 10.80 kg, 174.5± 7.2 cm), performed each exercise in a randomized order, using a repeated-measures design. Electromyographical (EMG) activity (sampling at 2,000 Hz) of the rectus abdominis (RA) and the erector spinae (ES) muscles was recorded throughout the duration of the exercises. Intraclass correlations demonstrated the highest levels of reliability for muscle activity during the isometric exercises; however, all exercises demonstrated high level of reliability (r = 0.764-0.998, p ≤ 0.01). The PB demonstrated significantly greater (p < 0.01) RA activity compared to all other exercises. The ES activity was significantly (p < 0.01) greater during the FS (1.010 ± 0.308 root mean square value [RMS (V)]) and SM (0.951 ± 0.217 RMS[V]) and compared to all other exercises, although there was no significant difference (p > 0.05) between the FS and the SM exercise. The PB may be the most suitable exercise for strengthening the RA, compared to dynamic exercises at a low to moderate load, because of a higher level of muscle activity. The FS may be a useful alternative to isometric exercises when strengthening the ES, because it results in slightly higher muscle activity levels when using only a light to moderate load. Because of the dynamic nature of the FS, this may also be more beneficial in transferring to activities of daily living and sporting environments.

Tendon structural and mechanical properties do not differ between genders in a healthy community-dwelling elderly population

Elderly women are reportedly at higher risk of falling than their male counterparts. Postural balance is highly associated with fall risk and is also correlated with tendon structural and mechanical properties. Gender differences in tendon properties could partly explain the discrepancy in fall risk. Thus the purpose of this study was to investigate the possible gender difference in tendon properties in the elderly. The properties of the patellar tendon of 55 elderly (men n = 27, aged 72 ± 1 years, women n = 28, aged 70 ± 1 years) participants were tested. Tendon stiffness (K), length (L), and cross‐sectional area (CSA) were measured using B‐mode ultrasonography, dynamometry, and electromyography during ramped isometric knee extensions. There were no significant differences (p > 0.05) between men and women in tendon stiffness (elderly men 550.9 ± 29.2 vs. women 502.9 ± 44.9 Nmm−1) or in Youngs modulus (elderly men 0.32 ± 0.02 vs. women 0.36 ± 0.04 GPa). This elderly group had similar tendon structural and mechanical properties. The comparable characteristics in gender‐specific tendon properties in an elderly population exhibiting similar lifestyle characteristics to the current sample may not explain the reports in the literature regarding increased fall risk in elderly women relative to that seen in men of a similar age.

Lower limb mechanical properties: determining factors and implications for performance.

Limb stiffness or musculotendinous stiffness (MTS) has previously been examined in relation to performance and characterized using a number of different methods. However, the fact that MTS shows only low to moderate correlations to performances may indicate a lack of understanding of this parameter. In addition to this, variation is seen between studies examining the same factors. To date, our understanding of MTS and its components are not complete and thus it is unclear which characteristic value represents the ideal index of stiffness as it relates to performance. Moreover, it is uncertain how MTS stiffness as a functional measure relates to performance, and also if there is an optimal amount of MTS stiffness for specific functions or tasks. The knowledge of the interplay of MTU stiffness as it relates to performance and injury risk is also poorly understood in that there is likely a disparity between levels of stiffness required to optimize performance and those required to minimize injury risk. The aim of this article is to review the literature as it describes the components of MTS and to discuss these in terms of their relationship to functional performance; consider adaptations of the MTU with training along with associated performance changes; highlight and discuss how stiffness may affect loading of the soft and bony tissues in terms of the MTU components and gender, with respect to risk of injury; discuss the apparent differences in the literature regarding associations of the various forms of stiffness index to function; suggest recommendations for training in light of adaptation of the muscle and tendon and injury risk in context of gender; and, finally, to highlight potential limitations of current methodologies and suggest further work to gain insight into the mechanisms of stiffness. It is hoped that by suggesting future work, a more detailed and comprehensive understanding of MTS will be gained, thus enabling appropriate interventions to optimally modify this parameter for specific requirements.