Stevan Wakefield Djuric
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Featured researches published by Stevan Wakefield Djuric.
Synthetic Communications | 1990
Thomas D. Penning; Stevan Wakefield Djuric; Richard A. Haack; Vincent J. Kalish; Julie M. Miyashiro; Bruce W. Rowell; Stella S. Yu
Abstract Lithium borohydride in the presence of one equivalent of water has been found to be an efficient agent for the reduction of sterically hindered acyloxazolidinones to the corresponding primary alcohol.
Inflammation Research | 1989
Donald J. Fretland; S. Levin; B.S. Tsai; Stevan Wakefield Djuric; D. L. Widomski; J. M. Zemaitis; R.L. Shone; R.F. Bauer
SC-41930, 7-[3-(4-acetyl-3-methoxy-2-propylphenoxy)-propoxy]-3,4-dihydro-8-propyl-2H-1-benzopyran-2-carboxylic acid, is a potentin vitro leukotriene-B4 (LTB4) receptor antagonist. LTB4 levels are elevated in colonic tissue of inflammatory bowel disease (IBD) patients which may account for the high degree of neutrophil (PMN) infiltration. The guinea pig acetic acid-induced colonic inflammation model has characteristics of IBD including PMN infiltration, edema, ulceration and necrosis. The model was used to evaluate the effect of SC-41930. SC-41930 was given orally, 30 min before and after intrarectal administration of 3% acetic acid. The PMN marker enzyme, myeloperoxidase, was measured along with histological evaluation to assess inflammation. Both parameters showed significantly less inflammation in SC-41930 treated animals with an oral ED50 of 20 mg/kg. These study results with an LTB4 receptor antagonist indicate a role for LTB4 in colonic inflammation and that an LTB4 receptor antagonist may be beneficial for treatment of IBD.
Prostaglandins | 1989
B.S. Tsai; Doreen Villani-Price; Robert H. Keith; J. M. Zemaitis; Raymond F. Bauer; R. Leonard; Stevan Wakefield Djuric; Robert L. Shone
SC-41930 was evaluated for effects on human neutrophil chemotaxis and degranulation. At concentrations up to 100 microM, SC-41930 alone exhibited no effect on neutrophil migration, but dose-dependently inhibited neutrophil chemotaxis induced by leukotriene B4 (LTB4) in a modified Boyden chamber. Concentrations of SC-41930 from 0.3 microM to 3 microM competitively inhibited LTB4-induced chemotaxis with a pA2 value of 6.35. While inactive at 10 microM against C5a-induced chemotaxis, SC-41930 inhibited N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced chemotaxis, with 10 times less potency than against LTB4-induced chemotaxis. SC-41930 inhibited [3H]LTB4 and [3H]fMLP binding to their receptor sites on human neutrophils with KD values of 0.2 microM and 2 microM, respectively. SC-41930 also inhibited neutrophil chemotaxis induced by 20-OH LTB or 12(R)-HETE. At concentrations up to 10 microM, SC-41930 alone did not cause neutrophil degranulation, but inhibited LTB4-induced degranulation in a noncompetitive manner. SC-41930 also inhibited fMLP- or C5a-induced degranulation, but was about 8 and 10 times less effective for fMLP and C5a, respectively. The results indicate that SC-41930 is a human neutrophil LTB4 receptor antagonist with greater specificity for LTB4 than for fMLP or C5a receptors.
Inflammation | 1990
Donald J. Fretland; D. L. Widomski; J. M. Zemaitis; R. E. Walsh; Stuart Levin; Stevan Wakefield Djuric; Robert L. Shone; B.S. Tsai; Timothy S. Gaginella
Neutrophil (PMNL) infiltration is a prominent feature of human psoriasis. Psoriatic skin lesions contain abnormally high amounts of leukotriene B4 (LTB4), itself a potent PMNL chemoattractant both in vivo and in vitro. SC-41930 {7-[3-(4-acetyl-3-methoxy-2-propylphenoxy)-propoxy]-3,4-dihydro-8-propyl-2H-1-benzo-pyran-2-carboxylic acid}, an orally active LTB4 receptor antagonist, was tested topically in models of skin inflammation induced by 200 nmol of the calcium ionophore A23187 or 200μg phorbol-12-myristate-13-acetate (PMA) applied topically to the guinea pig ear as assessed by ear weight, levels of the PMNL marker enzyme myeloperoxidase (MPO), and histological examination (PMA model) at 4 and 18 h respectively. When coapplied topically with A23187 or PMA, SC-41930 significantly inhibited epidermal inflammation with ED50 values of 0.6 and 4 mg, respectively. SC-41930 treatment also was associated with lowered dermal LTB4 levels in both models. The PMA-induced skin inflammation model also was assessed histologically and revealed acanthosis, edema, PMNL infiltration, and rete ridge prominence as long as 96 h after a single application that was completely inhibited by SC-41930 topical coapplication. Furthermore, oral treatment (40 mg/kg) significantly reduced edema and inflammatory cell infiltration in both models. These models possess many of the characteristics of human psoriasis, and agents such as SC-41930 that demonstrate activity in these models may well have therapeutic utility in the treatment of human psoriasis.
Tetrahedron Letters | 1988
Richard A. Haack; Thomas D. Penning; Stevan Wakefield Djuric; John A. Dziuba
Abstract trans -β-Bromostyrenes have been conveniently prepared in moderate to high yield in a one-pot two-step sequence. trans -1,2-Bis(tri-n-butylstannyl)ethylene underwent a smooth palladium(0) catalyzed coupling reaction with 0.5 equivalents of aromatic bromide or iodide to furnish a trans -β-stannylstyrene. This intermediate vinyl stannane, without isolation, was then converted to the substituted trans -bromostyrene on treatment with molecular bromine.
Bioorganic & Medicinal Chemistry Letters | 1995
Thomas D. Penning; Leslie J. Askonas; Stevan Wakefield Djuric; Richard A. Haack; Stella S. Yu; Marshall L. Michener; Gwen Grabowski Krivi; E.Yvonne Pyla
Abstract The hydroxamic acid-containing peptide kelatorphan, a known inhibitor of enkephalin-degrading enzymes, is a potent, non-competitive inhibitor of leukotriene A4 (LTA4) hydrolase. Analogs of kelatorphan were prepared and several significantly and selectively inhibited both the hydrolase and aminopeptidase activity of the enzyme.
Inflammation | 1989
Donald J. Fretland; D. L. Widomski; J. M. Zemaitis; Stevan Wakefield Djuric; Robert L. Shone
Leukotriene B4 (LTB4) is a proinflammatory product of arachidonic acid metabolism that has been implicated as a mediator in a number of inflammatory diseases. When injected intradermally into the cavine, LTB4 elicits a dose-dependent immigration (chemotaxis) of neutrophils (PMNs) into the injection sites as assessed by the presence of a neutrophil marker enzyme myeloperoxidase. SC-41930 {7-[3-(4-acetyl-3-methoxy-2-propylphenoxy)propoxy]-3,4-dihydro-8-propyl-2H-l-benzopyran-2-carboxylic acid, a potent LTB4 receptor antagonist inhibited the chemotactic actions of LTB4 when coadministered into the dermal site and when given intravenously or orally with ED50 values of 200 ng, 0.5 mg/kg, and 0.6 mg/ kg respectively. This compound may well have application in disease states, such as inflammatory bowel disease and psoriasis, where LTB4 is implicated as a proinflammatory mediator.
Prostaglandins Leukotrienes and Essential Fatty Acids | 1989
D.J. Fretland; D.L. Widomski; J. M. Zemaitis; B.S. Tsai; Stevan Wakefield Djuric; Thomas D. Penning; J.M. Miyashiro; R.F. Bauer
Psoriasis is a disease state characterized by epidermal proliferation, neutrophil infiltration, along with release of the proinflammatory mediators leukotriene-B4(LTB4) and 12(R)-hydroxyeicosatetraenoic acid [12(R)-HETE]. LTB4 and 12(R)-HETE are chemoattractant to the neutrophil, the latter approximately 1000x less potent. LTB4 and 12(R)-HETE are present in psoriatic scale, the latter in quantities so much greater than LTB4 that it is proposed as a primary mediator of neutrophil infiltration in psoriasis. 12(R)-HETE, synthesized in optically pure form by a new, shorter route, was injected into the dermis of the cavine, lapine, canine, mouse and rat. At doses up to 50 mu gm per intradermal site, 12(R)-HETE was chemoattractant to the neutrophil (as assessed by dermal myeloperoxidase levels) with response in the cavine greater than canine greater than lapine greater than mouse greater than rat.
Tetrahedron Letters | 1988
Stevan Wakefield Djuric; Julie M. Miyashiro; Thomas D. Penning
Abstract A practical enantioselective synthesis of 12(R)-HETE has been achieved from readily available starting materials. This process also constitutes a formal synthesis of Leukotriene B 4 .
Inflammation | 1995
Donald J. Fretland; C. P. Anglin; M. Bremer; Peter C. Isakson; D. L. Widomski; S. K. Paulson; Stephen H Docter; Stevan Wakefield Djuric; Thomas D. Penning; Stella S. Yu; J. P. McKearn
Leukotriene B4 (LTB4) and 12(R)-hydroxyeicosatetraenoic acid [12(R)-HETE] are proinflammatory products of arachidonic acid metabolism that have been implicated as mediators in a number of inflammatory diseases. When injected intradermally into the guinea pig, LTB4 and 12(R)-HETE elicit a dose-dependent migration (chemotaxis) of neutrophils (PMNs) into the injection sites as assessed by the presence of a neutrophil marker enzyme myeloperoxidase. SC-41930 {7-[3-(4-acetyl-3-methoxy-2-propylphenoxy)propoxyl]-3,4-dihydro-8-propyl-2H-1-benzopyran-2-carboxylic acid}, a first-generation LTB4 receptor antagonist, inhibited the chemotactic actions of LTB4 when given orally with an ED50 value of 1.7 mg/kg. The second-generation LTB4 receptor antagonist, SC-53228 [(+)-(S)-7-(3-{2-(cyclopropylmethyl)-3-methoxy-4-[(methylamino)carbonyl]phenoxy} propoxy)-3,4-dihydro-8-propyl-2H-1-benzopyran-2-propanoic acid], inhibited LTB4-induced chemotaxis when given intragastrically with an ED50 value of 0.07 mg/kg. Furthermore, SC-53228 inhibited 12(R)-HETE-induced granulocyte chemotaxis with an oral ED50 value of 5.8 mg/kg. When dosed orally over a range of 0.03–100 mg/kg, SC-53228 gaveCmax plasma concentrations of 0.015–41.1μg/ml. SC-53228 inhibited LTB4-primed membrane depolarization of human neutrophils with an IC50 value of 34 nM. As a potent LTB4 receptor antagonist, SC-53228 may well have application in the medical management of disease states such as asthma, rheumatoid arthritis, inflammatory bowel disease, contact dermatitis, and psoriasis, in which LTB4 and/or 12(R)-HETE are implicated as inflammatory mediators.