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Dive into the research topics where Steve J. Upton is active.

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Featured researches published by Steve J. Upton.


Clinical Microbiology Reviews | 2004

Cryptosporidium taxonomy: recent advances and implications for public health.

Lihua Xiao; Ronald Fayer; Una Ryan; Steve J. Upton

SUMMARY There has been an explosion of descriptions of new species of Cryptosporidium during the last two decades. This has been accompanied by confusion regarding the criteria for species designation, largely because of the lack of distinct morphologic differences and strict host specificity among Cryptosporidium spp. A review of the biologic species concept, the International Code of Zoological Nomenclature (ICZN), and current practices for Cryptosporidium species designation calls for the establishment of guidelines for naming Cryptosporidium species. All reports of new Cryptosporidium species should include at least four basic components: oocyst morphology, natural host specificity, genetic characterizations, and compliance with the ICZN. Altogether, 13 Cryptosporidium spp. are currently recognized: C. muris, C. andersoni, C. parvum, C. hominis, C. wrairi, C. felis, and C. cannis in mammals; C. baïleyi, C. meleagridis, and C. galli in birds; C. serpentis and C. saurophilum in reptiles; and C. molnari in fish. With the establishment of a framework for naming Cryptosporidium species and the availability of new taxonomic tools, there should be less confusion associated with the taxonomy of the genus Cryptosporidium. The clarification of Cryptosporidium taxonomy is also useful for understanding the biology of Cryptosporidium spp., assessing the public health significance of Cryptosporidium spp. in animals and the environment, characterizing transmission dynamics, and tracking infection and contamination sources.


International Journal for Parasitology | 2000

Epidemiology of Cryptosporidium : transmission, detection and identification

Ronald Fayer; U.M. Morgan; Steve J. Upton

There are 10 valid species of Cryptosporidium and perhaps other cryptic species hidden under the umbrella of Cryptosporidium parvum. The oocyst stage is of primary importance for the dispersal, survival, and infectivity of the parasite and is of major importance for detection and identification. Because most oocysts measure 4-6 microm, appear nearly spherical, and have obscure internal structures, there are few or no morphometric features to differentiate species and in vitro cultivation does not provide differential data as for bacteria. Consequently, we rely on a combination of data from three tools: morphometrics, molecular techniques, and host specificity. Of 152 species of mammals reported to be infected with C. parvum or an indistinguishable organism, very few oocysts have ever been examined using more than one of these tools. This paper reviews the valid species of Cryptosporidium, their hosts and morphometrics; the reported hosts for the human pathogen, C. parvum; the mechanisms of transmission; the drinking water, recreational water, and food-borne outbreaks resulting from infection with C. parvum; and the microscopic, immunological, and molecular methods used to detect and identify species and genotypes.


Journal of Biochemical and Biophysical Methods | 1994

A simple modification of Blum's silver stain method allows for 30 minite detection of proteins in polyacrylamide gels

Michael V. Nesterenko; Michael Tilley; Steve J. Upton

A simple and rapid protocol for silver staining of proteins following electrophoresis in polyacrylamide gels (PAGE) is described. We have reduced the number of steps in the procedure of Blum et al. (Electrophoresis (1987) 8, 93-99), and shortened fixation and washing times so that efficient detection of proteins can be achieved within 30 min. In common with more time-consuming silver-staining methods, the present protocol is capable of detecting nanogram quantities of proteins on a colorless background and is suitable for rapid screening of large numbers of samples.


Journal of Eukaryotic Microbiology | 2000

Cryptosporidium andersoni n. sp. (Apicomplexa: Cryptosporiidae) from Cattle, Bos taurus

David S. Lindsay; Steve J. Upton; Douglas S. Owens; U.M. Morgan; Jan R. Mead; Byron L. Blagburn

Abstract A new species of Cryptosporidium is described from the feces of domestic cattle, Bos taurus. Oocysts are structurally similar to those of Cryptosporidium muris described from mice but are larger than those of Cryptosporidium parvum. Oocysts of the new species are ellipsoidal, lack sporocysts, and measure 7.4 × 5.5 μm (range, 6.0–8.1 by 5.0–6.5 μm). The length to width ratio is 1.35 (range, 1.07–1.50). The colorless oocyst wall is < 1 μm thick, lacks a micropyle, and possesses a longitudinal suture at one pole. A polar granule is absent, whereas an oocyst residuum is present. Oocysts were passed fully sporulated and are not infectious to outbred, inbred immunocompetent or immunodeficient mice, chickens or goats. Recent molecular analyses of the rDNA 18S and ITS1 regions and heat-shock protein 70 (HSP-70) genes demonstrate this species to be distinct from C. muris infecting rodents. Based on transmission studies and molecular data, we consider the large form of Cryptosporidium infecting the abomasum of cattle to be a new species and have proposed the name Cryptosporidium andersoni n. sp. for this parasite.


International Journal for Parasitology | 2002

Redescription of Neospora caninum and its differentiation from related coccidia

J. P. Dubey; Bradd C. Barr; John R. Barta; Inge Bjerkås; Camilla Björkman; B L Blagburn; D D Bowman; D. Buxton; John Ellis; Bruno Gottstein; Andrew Hemphill; Dolores E. Hill; Daniel K. Howe; Mark C. Jenkins; Y. Kobayashi; Břetislav Koudela; Antoinette E. Marsh; Jens G. Mattsson; Milton M. McAllister; David Modrý; Yoshitaka Omata; L D Sibley; C.A. Speer; Alexander J. Trees; Arvid Uggla; Steve J. Upton; Diana J.L. Williams; David S. Lindsay

Neospora caninum is a protozoan parasite of animals, which before 1984 was misidentified as Toxoplasma gondii. Infection by this parasite is a major cause of abortion in cattle and causes paralysis in dogs. Since the original description of N. caninum in 1988, considerable progress has been made in the understanding of its life cycle, biology, genetics and diagnosis. In this article, the authors redescribe the parasite, distinguish it from related coccidia, and provide accession numbers to its type specimens deposited in museums.


Journal of Parasitology | 1985

The species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) infecting mammals.

Steve J. Upton; William L. Current

Oocysts of Cryptosporidium muris (Apicomplexa: Cryptosporidiidae) were obtained from the feces of naturally infected calves. Oocysts were fully sporulated in fresh feces, measured 7.4 X 5.6 (6.6 - 7.9 X 5.3 - 6.5) micron, and possessed a longitudinal suture along one pole of the oocyst wall. Morphologic and biologic evidence obtained from this study demonstrated that C. muris is a species distinct from Cryptosporidium parvum, which has smaller oocysts.


Systematic Parasitology | 1998

A revision of the taxonomy and nomenclature of the Eimeria spp. (Apicomplexa: Eimeriidae) from rodents in the Tribe Marmotini (Sciuridae)

Patricia G. Wilber; Donald W. Duszynski; Steve J. Upton; R.S. Seville; J.O. Corliss

All published papers on Eimeria spp. from rodents in the Marmotini Tribe are reviewed and each described species is evaluated in an historical context (i.e., beginning with the oldest description). Many of these species descriptions from marmotine rodents are invalid when considered within either the spirit or the letter of the International Code of Zoological Nomenclature. In addition, several previous authors erroneously assumed that Eimeria spp. of this host group were highly host-species specific. Thus, many eimerian species described to infect members of this tribe that were originally found in different hosts are morphologically indistinguishable, and several of these are considered to be conspecific. The names of those species that were most similar are synonymised and, using the guidelines of the International Code, the species for which the original descriptions were inadequate are considered to be species inquirendae (Ride et al., 1985, p. 264). The number of named eimerian species in the Marmotini is reduced from 40 to 26 valid species; these are E. airculensis, E. beckeri, E. beecheyi, E. berkinbaevi, E. callospermophili, E. citelli, E. cynomysis, E. deserticola, E. eutamiae, E. franklinii, E. hoffmeisteri, E. lateralis, E. ludoviciani, E. menzbieri, E. monacis, E. morainensis, E. os, E. ovata, E. pseudospermophili, E. spermophili, E. surki, E. tamiasciuri, E. vilasi, E. volgensis and E. yukonensis, and one new name is included. Nine junior synonyms are identified and five species inquirendae and one nomen nudum are discussed. Where needed, the identification errors in the published literature are clarified.


Insect Biochemistry and Molecular Biology | 1996

Luminal proteinases from Plodia interpunctella and the hydrolysis of Bacillus thuringiensis CryIA(c) protoxin.

Brenda Oppert; Karl J. Kramer; Donovan E. Johnson; Steve J. Upton; William H. McGaughey

The ability of proteinases in gut extracts of the Indianmeal moth, Plodia interpunctella, to hydrolyze Bacillus thuringiensis (Bt) protoxin, casein, and rho-nitroanilide substrates was investigated. A polyclonal antiserum to protoxin CryIA(c) was used in Western blots to demonstrate slower protoxin processing by gut enzymes from Bt subspecies entomocidus-resistant larvae than enzymes from susceptible or kurstaki-resistant strains. Enzymes from all three strains hydrolyzed N-alpha-benzoyl-L-arginine rho-nitroanilide, N-succinyl-ala-ala-pro-phenylalanine rho-nitroanilide, and N-succinyl-ala-ala-pro-leucine rho-nitroanilide. Zymograms and activity blots were used to estimate the apparent molecular masses, number of enzymes, and relative activities in each strain. Several serine proteinase inhibitors reduced gut enzyme activities, with two soybean trypsin inhibitors, two potato inhibitors, and chymostatin the most effective in preventing protoxin hydrolysis.


Annals of Tropical Medicine and Parasitology | 1996

Efficacy of 101 antimicrobials and other agents on the development of Cryptosporidium parvum in vitro

Keith M. Woods; Michael V. Nesterenko; Steve J. Upton

An in-situ ELISA was used as a primary screen to test the effects of 101 antimicrobials and other agents on the development of Cryptosporidium parvum in vitro. Over 40 of the compounds displayed some form of anticryptosporidial activity, and dose-response curves were generated for 40 of these. The in-situ ELISA makes a highly effective primary, pharmaceutical screen for C parvum, to be used prior to more detailed microscopical, toxicological or in-vivo assays.


International Journal for Parasitology | 1999

A structural study of the Neospora caninum oocyst

David S. Lindsay; Steve J. Upton; J. P. Dubey

Oocysts of Neospora caninum were collected from the faeces of a dog fed mouse brains containing tissue cysts of the NC-beef strain of N. caninum. Sporulated oocysts were spherical to subspherical, and were 11.7x11.3 microm. The length/width ratio was 1.04. No micropyle or oocyst residuum was present. Polar granules were not present, although occasionally tiny refractile granules were seen among sporocysts. Sporocysts were ellipsoidal, did not contain a Stieda body, and were 8.4x6.1 microm. The length/width ratio for sporocysts was 1.37. A spherical or subspherical sporocyst residuum was present, and was composed of a cluster of small, compact granules of 4.3x3.9 microm, or was represented by many dispersed granules of similar size. Sporozoites were elongate and 7.0-8.0x2.0-3.0 microm in situ. No refractile bodies were present and the nucleus was centrally or slightly posteriorly located. The features of the oocyst of N. caninum are similar to those of Hammondia heydorni oocysts from dog faeces and Toxoplasma gondii and Hammondia hammondi oocysts from cat faeces.

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Chris T. McAllister

Eastern Oklahoma State College

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