Steven H. Nye
Regeneron
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Featured researches published by Steven H. Nye.
Cell | 1991
Teri G. Boulton; Steven H. Nye; David Robbins; Nancy Y. Ip; Elizabeth Radzlejewska; Sharon D. Morgenbesser; Ronald A. DePinho; Nikos Panayotatos; Melanie H. Cobb; George D. Yancopoulos
We recently described the purification and cloning of extracellular signal-regulated kinase 1 (ERK1), which appears to play a pivotal role in converting tyrosine phosphorylation into the serine/threonine phosphorylations that regulate downstream events. We now describe cloning and characterization of two ERK1-related kinases, ERK2 and ERK3, and provide evidence suggesting that there are additional ERK family members. At least two of the ERKs are activated in response to growth factors; their activations correlate with tyrosine phophorylation, but also depend on additional modifications. Transcripts corresponding to the three cloned ERKs are distinctly regulated both in vivo and in a differentiating cell line. Thus, this family of kinases may serve as intermediates that depend on tyrosine phosphorylation to activate serine/threonine phosphorylation cascades. Individual family members may mediate responses in different developmental stages, in different cell types, or following exposure to different extracellular signals.
Cell | 1992
Nancy Y. Ip; Steven H. Nye; Teri G. Boulton; Samuel Davis; Tetsuya Taga; Yanping Li; Susan J. Birren; Kiyoshi Yasukawa; Tadamitsu Kishimoto; David J. Anderson; Neil Stahl; George D. Yancopoulos
Ciliary neurotrophic factor (CNTF) has a variety of actions within the nervous system. While some of the actions of leukemia inhibitory factor (LIF) on neurons resemble those of CNTF, LIF also has broad actions outside of the nervous system that in many cases mimic those of interleukin-6 (IL-6). Comparison of the tyrosine phosphorylations and gene activations induced by CNTF and LIF in neuron cell lines reveals that they are indistinguishable and also very similar to signaling events that characterize LIF and IL-6 responses in hematopoietic cells. We provide a basis for the overlapping actions of these three factors by demonstrating that the shared CNTF and LIF signaling pathways involve the IL-6 signal transducing receptor component gp130. Thus, the receptor system for CNTF is surprisingly unlike those used by the nerve growth factor family of neurotrophic factors, but is instead related to those used by a subclass of hematopoietic cytokines.
Cell | 1991
David J. Glass; Steven H. Nye; Petros A. Hantzopoulos; Mary J. Macchi; Stephen P. Squinto; Mitchell Goldfarb; George D. Yancopoulos
The neurotrophins (nerve growth factor [NGF], brain-derived neurotrophic factor [BDNF], neurotrophin-3 [NT-3], and neurotrophin-4 [NT-4] ) have been defined by their ability to support neuronal survival. These factors utilize the Trk family of receptor tyrosine kinases, perhaps in conjunction with a second component known as the low affinity NGF receptor (LNGFR). Here we demonstrate that TrkB mediates survival and proliferation in response to both BDNF and NT-3 when expressed in a particular strain of NIH 3T3 fibroblasts, with BDNF the more potent ligand. Furthermore, the BDNF dose dependency displayed by these TrkB-expressing fibroblasts is similar to that required to support the survival of primary neurons. The LNGFR is not expressed in our fibroblast system, implying that this receptor is not essential for responses to physiological concentrations of the neurotrophins. We discuss our findings in the context of neurotrophin signaling pathways and mechanisms of neuronal cell death.
Journal of Clinical Investigation | 1996
Eileen A. Elliott; Hugh I. McFarland; Steven H. Nye; Roxanne Cofiell; Todd M. Wilson; James A. Wilkins; Stephen P. Squinto; Louis A. Matis; John P. Mueller
It has been shown that peripheral T cell tolerance can be induced by systemic antigen administration. We have been interested in using this phenomenon to develop antigen-specific immunotherapies for T cell-mediated autoimmune diseases. In patients with the demyelinating disease multiple sclerosis (MS), multiple potentially autoantigenic epitopes have been identified on the two major proteins of the myelin sheath, myelin basic protein (MBP) and proteolipid protein (PLP). To generate a tolerogenic protein for the therapy of patients with MS, we have produced a protein fusion between the 21.5-kD isoform of MBP (MBP21.5) and a genetically engineered form of PLP (deltaPLP4). In this report, we describe the effects of treatment with this agent (MP4) on clinical disease in a murine model of demyelinating disease, experimental autoimmune encephalomyelitis (EAE). Treatment of SJL/J mice with MP4 after induction of EAE either by active immunization or by adoptive transfer of activated T cells completely prevented subsequent clinical paralysis. Importantly, the administration of MP4 completely suppressed the development of EAE initiated by the cotransfer of both MBP- and PLP-activated T cells. Prevention of clinical disease after the intravenous injection of MP4 was paralleled by the formation of long-lived functional peptide-MHC complexes in vivo, as well as by a significant reduction in both MBP- and PLP-specific T cell proliferative responses. Mice treated with MP4 were resistant to disease when rechallenged with an encephalitogenic PLP peptide emulsified in CFA, indicating that MP4 administration had a prolonged effect in vivo. Administration of MP4 was also found to markedly ameliorate the course of established clinical disease. Finally, MP4 therapy was equally efficacious in mice defective in Fas expression. These results support the conclusion that MP4 protein is highly effective in suppressing disease caused by multiple neuroantigen epitopes in experimentally induced demyelinating disease.
Proceedings of the National Academy of Sciences of the United States of America | 1992
Nancy Y. Ip; C F Ibáñez; Steven H. Nye; Joyce McClain; P. Jones; David R. Gies; Leonardo Belluscio; M. Le Beau; rd R Espinosa; Stephen P. Squinto
Molecular Biology of the Cell | 1992
Steven H. Nye; Stephen P. Squinto; David J. Glass; Trevor N. Stitt; Petros A. Hantzopoulos; Mary J. Macchi; N S Lindsay; Nancy Y. Ip; George D. Yancopoulos
Archive | 1992
Stephen P. Squinto; Steven H. Nye; George D. Yancopoulos
Archive | 1992
Nancy Ip; Nikos Panayotatos; Thomas H. Aldrich; Samuel Davis; Daniel Everdeen; Steven H. Nye; Stephen P. Squinto; Neil Stahl; Joanne C. Conover; George D. Yancopoulos
Archive | 2005
Mark J. Evans; Louis A. Matis; Eileen Elliott Mueller; Steven H. Nye; Scott Rollins; Russell P. Rother; Jeremy P. Springhorn; Stephen P. Squinto; Thomas C. Thomas; Yi Wang; James A. Wilkins; ミューラー、アイリーン・エリオット; ウィルキンズ、ジェイムズ・エイ; スプリングホーン、ジェレミー・ピー; ローリンズ、スコット; ニュー、スティーブン・エイチ; スクウィント、ステファン・ピー; トーマス、トーマス・シー; エバンス、マーク・ジェイ; ローサー、ラッセル・ピー; マティス、ルイス; イー ワン
Archive | 1996
John P. Mueller; Michael J. Lenardo; Henry F. McFarland; Louis A. Matis; Eileen Elliott Mueller; Steven H. Nye; Clara M. Pelfrey; Stephen P. Squinto; James A. Wilkins