Su Xiaohua

Multiple transgenes Populus xeuramericana ‘Guariento’ plants obtained by biolistic bombardment

A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana ‘Guariento’ using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana ‘Guariento’ and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic popular plants, and these plants grew well on coastal saline land.

Foliar Carbon Isotope Composition (δ13C) and Water Use Efficiency of Different Populus deltoids Clones Under Water Stress

Foliar carbon isotope composition (δ13C), total dry biomass, and long-term water use efficiency (WUEL) of 12 Populus deltoids clones were studied under water stress in a greenhouse. Total dry biomass of clones decreased greatly, while δ13C increased. Single-element variance analysis in the same water treatment indicated that WUEL difference among clones was significant. Clones J2, J6, J7, J8, and J9 were excellent with high WUEL. Extremely significant δ13C differences among water treatments and clones were revealed by two-element variance analysis. Water proved to be the primary factor affecting δ13C under water stress. It showed that there was a good positive correlation between δ13C and WUEL in the same water treatment, and that a high WUEL always coincided with a high δ13C. δ13C might be a reliable indirect index to estimate WUEL among P. deltoids clones.

Regeneration of vgb -transgenic poplar ( Populus alba × P. glandulosa ) and the primary observation of growth

Increasing the growth rate is especially important for low-quality wood applications, so this has become an important goal in poplar breeding. The present study describes the transfer of Vitreoscilla haemoglobin (VHb) gene ( vgb ) driven by constitutive promoters, by Agrobacterium tumefaciens into poplar ( Populus alba × P. glandulosa ). From about 450 leaf discs used for transformation, 60 Kan-resistant plants were obtained, and 52 proved to be true transgenic plants. The transgenic nature of these plants was confirmed by polymerase chain reaction (PCR) amplification and Southern dot blot hybridization. The expression of vgb gene in transgenic plants was confirmed by reverse transcriptase-PCR (RT-PCR). The performance of the transgenic lines was evaluated during the first year of growth in a greenhouse. These plants showed no significant stable morphological differences from the untransformed plants. Among them, three vgb -transgenic lines exhibited noticeably higher growth rates in terms of height and diameter.

干旱胁迫对转 JERF36 银中杨苗木叶片解剖结构及光合特性的影响

目的 以转JERF36银中杨（ABJ01）和非转基因银中杨（9#）为试验材料，开展干旱胁迫对2个株系苗高生长、叶片形态解剖结构、光合特性的影响研究，以期为转基因杨树的抗旱性评价提供参考，并为转基因杨树的推广应用提供科学依据。 方法 于2015年6月底，苗高约45 cm时，选取生长一致的苗木进行土壤干旱胁迫试验。胁迫程度分为3个梯度：正常供水、中度胁迫、重度胁迫，土壤含水量分别控制在田间持水量的60%~80%，40%~60%，20%~40%，胁迫时间为30天。 结果 干旱胁迫下，2个株系的苗高生长均受到抑制，随着胁迫程度的加剧，受抑制程度增大，但ABJ01受抑制程度较低，重度胁迫下其苗高显著高于9#。叶片形态数据显示，与正常供水相比，干旱胁迫处理后，2个株系的单叶面积显著降低，说明干旱胁迫抑制杨树叶片生长；中度、重度胁迫下9#的单叶面积均显著低于ABJ01，表明ABJ01叶片生长受抑制程度低。叶片解剖结构数据显示，ABJ01和9#的叶表皮细胞生长和叶肉细胞生长均受干旱胁迫的抑制，但ABJ01受抑制程度较低。中度胁迫下，ABJ01的叶片上、下表皮厚度分别比9#显著高出5.55%和4.70%，栅栏组织厚度比9#显著高出6.17%，海绵组织厚度和叶片组织疏松度（SR）则分别比9#显著降低12.35%和12.38%；重度胁迫下ABJ01的叶片上、下表皮厚度分别比9#显著高出16.27%和10.58%，海绵组织厚度和SR则分别比9#显著降低11.71%和11.58%。ABJ01具有更发达的栅栏组织和相对少的海绵组织，这有助于CO2向光合场所的传导，维持叶片较高的净光合速率（Pn），以适应干旱胁迫条件。光合生理数据显示，干旱胁迫下，ABJ01的Pn显著高于非转基因株系9#（10.50%~18.97%），说明干旱胁迫下ABJ01具有更强的光合能力。正常供水下，2个株系的气孔导度（G3）、最大光化学效率（Fv/Fm）差异不显著，干旱胁迫下转基因株系ABJ01的Gs、Fv/Fm下降幅度比非转基因株系9#小，说明ABJ01受干旱胁迫影响程度较低；ABJ01的蒸腾速率（Tr）小于9#，表明ABJ01在干旱胁迫下具有更强的保水能力。ABJ01的叶片叶绿素a、叶绿素b和总叶绿素含量较9#高，Fv/Fm值较9#高，说明转基因株系维持叶绿素含量稳定的能力较强且光系统受损伤程度小。 结论 外源基因JERF36可能通过影响叶片结构发育提高转基因银中杨在干旱胁迫下的气体交换能力和保水能力，从而增强转基因银中杨的抗旱能力。